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Conformation of a purified "spontaneously" inserting thylakoid membrane protein precursor in aqueous solvent and detergent micelles

Woolhead, Cheryl A., Mant, Alexandra, Kim, Soo Jung, Robinson, Colin and Rodger, Alison (2001) Conformation of a purified "spontaneously" inserting thylakoid membrane protein precursor in aqueous solvent and detergent micelles The Journal of Biological Chemistry, 276, (18), pp. 14607-14613. (doi:10.1074/jbc.M009600200).

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Subunit W of photosystem II (PsbW) is a single-span thylakoid membrane protein that is synthesized with a cleavable hydrophobic signal peptide and integrated into the thylakoid membrane by an apparently spontaneous mechanism. In this study, we have analyzed the secondary structure of the pre-protein at early stages of the insertion pathway, using purified recombinant pre-PsbW. We show that the protein remains soluble in Tris buffer after removal of detergent. Under these conditions pre-PsbW contains no detectable alpha -helix, whereas substantial alpha -helical structure is present in SDS micelles. In aqueous buffer, the tryptophan fluorescence emission characteristics are intermediate between those of solvent-exposed and hydrophobic environments, suggesting the formation of a partially folded structure. If denaturants are excluded from the purification protocol, pre-PsbW purifies instead as a 180-kDa oligomer with substantial alpha -helical structure. Mature-size PsbW was prepared by removal of the presequence, and we show that this protein also contains alpha -helix in detergent but in lower quantities than the pre-protein. We therefore propose that pre-PsbW contains alpha -helical structure in both the mature protein and the signal peptide in nonpolar environments. We propose that pre-PsbW acquires its alpha -helical structure only during the later, membrane-bound stages of the insertion pathway, after which it forms a "helical hairpin"-type loop intermediate in the thylakoid membrane.

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Published date: 2001


Local EPrints ID: 26669
PURE UUID: a2dd8bd6-426f-46d5-b685-dc603a3d791b

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Date deposited: 24 Apr 2006
Last modified: 17 Jul 2017 16:06

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Author: Cheryl A. Woolhead
Author: Alexandra Mant
Author: Soo Jung Kim
Author: Colin Robinson
Author: Alison Rodger

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