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Negative DEP traps for single cell immobilisation

Record type: Article

We present a novel design of micron-sized particle trap that uses negative dielectrophoresis (nDEP) to trap cells in high conductivity physiological media. The design is scalable and suitable for trapping large numbers of single cells. Each trap has one electrical connection and the design can be extended to produce a large array. The trap consists of a metal ring electrode and a surrounding ground plane, which create a closed electric field cage in the centre. The operation of the device was demonstrated by trapping single latex spheres and HeLa cells against a moving fluid. The dielectrophoretic holding force was determined experimentally by measuring the displacement of a trapped particle in a moving fluid. This was then compared with theory by numerically solving the electric field for the electrodes and calculating the trapping force, demonstrating good agreement. Analysis of the 80 µm diameter trap showed that a 15.6 µm diameter latex particle could be held with a force of 23 pN at an applied voltage of 5 V peak–peak

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Citation

Thomas, Rupert, Morgan, Hywel and Green, Nicolas G (2009) Negative DEP traps for single cell immobilisation Lab on a Chip, 9, pp. 1534-1540. (doi:10.1039/b819267g).

More information

Published date: January 2009
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 267221
URI: http://eprints.soton.ac.uk/id/eprint/267221
ISSN: 1473-0197
PURE UUID: 0b1ae690-3156-4b64-a2d4-a14fcc674201
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Nicolas G Green: ORCID iD orcid.org/0000-0001-9230-4455

Catalogue record

Date deposited: 23 Apr 2010 13:13
Last modified: 18 Jul 2017 07:06

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Contributors

Author: Rupert Thomas
Author: Hywel Morgan ORCID iD
Author: Nicolas G Green ORCID iD

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