The University of Southampton
University of Southampton Institutional Repository

Negative DEP traps for single cell immobilisation

Thomas, Rupert, Morgan, Hywel and Green, Nicolas G (2009) Negative DEP traps for single cell immobilisation Lab on a Chip, 9, pp. 1534-1540.

Record type: Article


We present a novel design of micron-sized particle trap that uses negative dielectrophoresis (nDEP) to trap cells in high conductivity physiological media. The design is scalable and suitable for trapping large numbers of single cells. Each trap has one electrical connection and the design can be extended to produce a large array. The trap consists of a metal ring electrode and a surrounding ground plane, which create a closed electric field cage in the centre. The operation of the device was demonstrated by trapping single latex spheres and HeLa cells against a moving fluid. The dielectrophoretic holding force was determined experimentally by measuring the displacement of a trapped particle in a moving fluid. This was then compared with theory by numerically solving the electric field for the electrodes and calculating the trapping force, demonstrating good agreement. Analysis of the 80 µm diameter trap showed that a 15.6 µm diameter latex particle could be held with a force of 23 pN at an applied voltage of 5 V peak–peak

PDF b819267g.pdf - Other
Download (2MB)

More information

Published date: January 2009
Organisations: Electronics & Computer Science


Local EPrints ID: 267221
ISSN: 1473-0197
PURE UUID: 0b1ae690-3156-4b64-a2d4-a14fcc674201
ORCID for Hywel Morgan: ORCID iD
ORCID for Nicolas G Green: ORCID iD

Catalogue record

Date deposited: 23 Apr 2010 13:13
Last modified: 30 Sep 2017 05:00

Export record


Author: Rupert Thomas
Author: Hywel Morgan ORCID iD
Author: Nicolas G Green ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.