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Single cell imaging using electrical impedance tomography

Single cell imaging using electrical impedance tomography
Single cell imaging using electrical impedance tomography
Electrical impedance spectroscopy is a non-invasive technology for characterizing the dielectric properties of biological tissues and cells. Electrical impedance tomography extends impedance measurements from one dimension to two or three dimensions. Impedance measurements are performed across multiple electrodes, mapping the conductivity distribution within an object. In this paper, electrical impedance tomography is used to image a multi-nucleated cell, Physarum polycephalum, growing on agar gel in a miniaturized chip containing a circular 16-electrode array. An impedance analyzer combined with a USB-controlled multiplexing circuit board is used to perform adjacent impedance measurements. An open source software, EIDORS is used for image reconstruction and the system is evaluated using finite element modeling. Experimentally, a preliminary reconstructed image of Physarum is shown. The system has the potential to monitor kinetics of cells culture.
Sun, Tao
b2f8e932-a7e6-4fe7-94dd-5c4ce725eacb
Tsuda, Soichiro
85070e3b-3f9b-4183-ac62-13e273eee0a8
Zauner, Klaus-Peter
c8b22dbd-10e6-43d8-813b-0766f985cc97
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Sun, Tao
b2f8e932-a7e6-4fe7-94dd-5c4ce725eacb
Tsuda, Soichiro
85070e3b-3f9b-4183-ac62-13e273eee0a8
Zauner, Klaus-Peter
c8b22dbd-10e6-43d8-813b-0766f985cc97
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Sun, Tao, Tsuda, Soichiro, Zauner, Klaus-Peter and Morgan, Hywel (2009) Single cell imaging using electrical impedance tomography. 4th IEEE international conference of Nano/Micro Engineered and Molecular Systems, China. 05 - 08 Jan 2009.

Record type: Conference or Workshop Item (Other)

Abstract

Electrical impedance spectroscopy is a non-invasive technology for characterizing the dielectric properties of biological tissues and cells. Electrical impedance tomography extends impedance measurements from one dimension to two or three dimensions. Impedance measurements are performed across multiple electrodes, mapping the conductivity distribution within an object. In this paper, electrical impedance tomography is used to image a multi-nucleated cell, Physarum polycephalum, growing on agar gel in a miniaturized chip containing a circular 16-electrode array. An impedance analyzer combined with a USB-controlled multiplexing circuit board is used to perform adjacent impedance measurements. An open source software, EIDORS is used for image reconstruction and the system is evaluated using finite element modeling. Experimentally, a preliminary reconstructed image of Physarum is shown. The system has the potential to monitor kinetics of cells culture.

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More information

Published date: 6 January 2009
Additional Information: Event Dates: 5th- 8th, January
Venue - Dates: 4th IEEE international conference of Nano/Micro Engineered and Molecular Systems, China, 2009-01-05 - 2009-01-08
Organisations: Agents, Interactions & Complexity, Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 267590
URI: https://eprints.soton.ac.uk/id/eprint/267590
PURE UUID: 75747b77-8d18-4b51-8614-f8d4e99e9af7
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 18 Jun 2009 19:25
Last modified: 20 Jul 2019 00:59

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Contributors

Author: Tao Sun
Author: Soichiro Tsuda
Author: Klaus-Peter Zauner
Author: Hywel Morgan ORCID iD

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