The University of Southampton
University of Southampton Institutional Repository

Human CD1-restricted T cell recognition of lipids from pollens

Human CD1-restricted T cell recognition of lipids from pollens
Human CD1-restricted T cell recognition of lipids from pollens
Plant pollens are an important source of environmental antigens that stimulate allergic responses. In addition to acting as vehicles for foreign protein antigens, they contain lipids that incorporate saturated and unsaturated fatty acids, which are necessary in the reproduction of higher plants. The CD1 family of nonpolymorphic major histocompatibility complex–related molecules is highly conserved in mammals, and has been shown to present microbial and self lipids to T cells. Here, we provide evidence that pollen lipids may be recognized as antigens by human T cells through a CD1-dependent pathway.
Among phospholipids extracted from cypress grains, phosphatidyl-choline and phosphatidyl-ethanolamine were able to stimulate the proliferation of T cells from cypress-sensitive subjects. Recognition of phospholipids involved multiple cell types, mostly CD4+ T cell receptor for antigen (TCR)ß+, some CD4–CD8– TCR+, but rarely V24i+ natural killer–T cells, and required CD1a+ and CD1d+ antigen presenting cell. The responding T cells secreted both interleukin (IL)-4 and interferon-, in some cases IL-10 and transforming growth factor-ß, and could provide help for immunoglobulin E (IgE) production. Responses to pollen phospholipids were maximally evident in blood samples obtained from allergic subjects during pollinating season, uniformly absent in Mycobacterium tuberculosis–exposed health care workers, but occasionally seen in nonallergic subjects. Finally, allergic, but not normal subjects, displayed circulating specific IgE and cutaneous weal and flare reactions to phospholipids.
0022-1007
295-308
Agea, Elisabetta
c9537ce6-7cc1-4d03-9449-31f770efe298
Russano, Anna
53b4a569-2d4a-406f-b1f4-de4575933390
Bistoni, Onelia
893e82d5-7b4c-46dd-a11c-65c775c7d21d
Mannucci, Roberta
d86d1d1c-5382-49f9-b775-ff214be7711d
Nicoletti, Ildo
6fe9460b-e149-4688-a2cb-7222e550d779
Corazzi, Lanfranco
2353df1c-ec76-4986-8c18-25ab8006819b
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
DeLibero, Gennaro
dcc26587-8959-446c-902c-8cacf6f3d7c2
Porcelli, Steven A.
520e54b7-c341-4e81-9d10-7751598596ac
Spinozzi, Fabrizio
515ccc52-98d5-4e1c-9d21-8d777c7651cd
Agea, Elisabetta
c9537ce6-7cc1-4d03-9449-31f770efe298
Russano, Anna
53b4a569-2d4a-406f-b1f4-de4575933390
Bistoni, Onelia
893e82d5-7b4c-46dd-a11c-65c775c7d21d
Mannucci, Roberta
d86d1d1c-5382-49f9-b775-ff214be7711d
Nicoletti, Ildo
6fe9460b-e149-4688-a2cb-7222e550d779
Corazzi, Lanfranco
2353df1c-ec76-4986-8c18-25ab8006819b
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
DeLibero, Gennaro
dcc26587-8959-446c-902c-8cacf6f3d7c2
Porcelli, Steven A.
520e54b7-c341-4e81-9d10-7751598596ac
Spinozzi, Fabrizio
515ccc52-98d5-4e1c-9d21-8d777c7651cd

Agea, Elisabetta, Russano, Anna, Bistoni, Onelia, Mannucci, Roberta, Nicoletti, Ildo, Corazzi, Lanfranco, Postle, Anthony D., DeLibero, Gennaro, Porcelli, Steven A. and Spinozzi, Fabrizio (2005) Human CD1-restricted T cell recognition of lipids from pollens. The Journal of Experimental Medicine, 202 (2), 295-308. (doi:10.1084/jem.20050773).

Record type: Article

Abstract

Plant pollens are an important source of environmental antigens that stimulate allergic responses. In addition to acting as vehicles for foreign protein antigens, they contain lipids that incorporate saturated and unsaturated fatty acids, which are necessary in the reproduction of higher plants. The CD1 family of nonpolymorphic major histocompatibility complex–related molecules is highly conserved in mammals, and has been shown to present microbial and self lipids to T cells. Here, we provide evidence that pollen lipids may be recognized as antigens by human T cells through a CD1-dependent pathway.
Among phospholipids extracted from cypress grains, phosphatidyl-choline and phosphatidyl-ethanolamine were able to stimulate the proliferation of T cells from cypress-sensitive subjects. Recognition of phospholipids involved multiple cell types, mostly CD4+ T cell receptor for antigen (TCR)ß+, some CD4–CD8– TCR+, but rarely V24i+ natural killer–T cells, and required CD1a+ and CD1d+ antigen presenting cell. The responding T cells secreted both interleukin (IL)-4 and interferon-, in some cases IL-10 and transforming growth factor-ß, and could provide help for immunoglobulin E (IgE) production. Responses to pollen phospholipids were maximally evident in blood samples obtained from allergic subjects during pollinating season, uniformly absent in Mycobacterium tuberculosis–exposed health care workers, but occasionally seen in nonallergic subjects. Finally, allergic, but not normal subjects, displayed circulating specific IgE and cutaneous weal and flare reactions to phospholipids.

Full text not available from this repository.

More information

Published date: 2005

Identifiers

Local EPrints ID: 26898
URI: http://eprints.soton.ac.uk/id/eprint/26898
ISSN: 0022-1007
PURE UUID: fb8d218d-c204-40ff-bc7b-e4103171dab0
ORCID for Anthony D. Postle: ORCID iD orcid.org/0000-0001-7361-0756

Catalogue record

Date deposited: 25 Apr 2006
Last modified: 03 Dec 2019 02:08

Export record

Altmetrics

Contributors

Author: Elisabetta Agea
Author: Anna Russano
Author: Onelia Bistoni
Author: Roberta Mannucci
Author: Ildo Nicoletti
Author: Lanfranco Corazzi
Author: Gennaro DeLibero
Author: Steven A. Porcelli
Author: Fabrizio Spinozzi

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×