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Tear and conjunctival changes during the allergen-induced early-and late-phase responses

Bacon, Annette S., Ahluwalia, Poonam, Irani, Anne-Marie, Schwartz, Lawrence B., Holgate, Stephen T., Church, Martin K. and McGill, James I. (2000) Tear and conjunctival changes during the allergen-induced early-and late-phase responses Journal of Allergy and Clinical Immunology, 106, (5), pp. 948-954. (doi:10.1067/mai.2000.110930).

Record type: Article

Abstract

Background: Allergic eye disease is common, but little is known about the underlying disease mechanisms. Conjunctival allergen challenge causes symptoms similar to those of seasonal allergic conjunctivitis and is a useful model to study.
Objective: We have used allergen challenge to investigate the course of the ocular response, tear inflammatory mediators, tissue adhesion protein expression, and cellular infiltration. Methods: Eighteen atopic patients and 4 nonatopic control subjects were challenged with extracted mixed grass or Dermatophagoides pteronyssinus in one eye and control vehicle in the other. The clinical response was recorded, and tears were collected over a 6-hour period. Conjunctival biopsy specimens were taken from the challenged eye at 6 or 24 hours.
Results: An early-phase response (maximal at 20 minutes) showed a significant increase in tear histamine and tryptase levels, reducing to control levels again by 40 minutes. At 6 hours, a late-phase response occurred with increased symptoms, a second peak of tear histamine and eosinophil cationic protein but not tryptase, upregulation of the adhesion molecules E-selectin and intercellular adhesion molecule, and a cellular infiltrate of mast cells, neutrophils, eosinophils, macrophages, and basophils, with T cells increased only in bulbar biopsy specimens.
Conclusions: The early peaks of tear histamine plus tryptase indicate that the mast cell is responsible for the early-phase response, but basophils may be involved in the late-phase response. Both tear and biopsy findings underline the significance of the late-phase response as the transition between a type I response and clinical disease.

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More information

Published date: 2000
Additional Information: Dermatologic and ocular diseases

Identifiers

Local EPrints ID: 26921
URI: http://eprints.soton.ac.uk/id/eprint/26921
ISSN: 0091-6749
PURE UUID: 169ce9f6-2780-4d05-8e26-e40864e678bd

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Date deposited: 26 Apr 2006
Last modified: 17 Jul 2017 16:06

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Contributors

Author: Annette S. Bacon
Author: Poonam Ahluwalia
Author: Anne-Marie Irani
Author: Lawrence B. Schwartz
Author: Martin K. Church
Author: James I. McGill

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