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Modification of T-cell receptor Vβ repertoire in response to allergen stimulation in peanut allergy

Modification of T-cell receptor Vβ repertoire in response to allergen stimulation in peanut allergy
Modification of T-cell receptor Vβ repertoire in response to allergen stimulation in peanut allergy
Background: Peanut is one of the most common foods causing allergic reactions and is the most common cause of fatal and near-fatal food-related anaphylaxis. Little is known of the immunologic mechanisms that underlie peanut allergy. Objectives: In this study we examined clonality of the T-cell response (TCR) to peanut in MHC class II identical, peanut allergy–discordant sibling pairs.
Methods: Four sibling pairs were investigated. The TCR repertoire was analyzed before and after in vitro stimulation of PBMCs with crude peanut or PHA, as control for general/nonspecific reactivity. Eighteen TCR-Vβ families were examined by flow cytometry. Where significant differences in incidence of particular TCR-Vβ families were observed, PCR familyspecific cDNA amplification and gene scanning were performed.
Results: After stimulation with peanut, no selective expansion of any TCR-Vβ subpopulation was observed with flow cytometry, in either the peanut-allergic or nonallergic siblings, with the exception of 1 peanut-allergic subject who demonstrated a significant increase of TCR-Vβ11+ cells (0.3%-5.9% of the total CD3+ cells). However, gene scanning revealed predominant single-size PCR products for TCRBV11 in all peanut-allergic subjects after peanut stimulation. TCRBV11 polyclonality was observed in allergic and nonallergic subjects before peanut stimulation and in nonallergic subjects after peanut stimulation. In comparison, all subjects, before and after stimulation with peanut, showed polyclonality for TCRBV2.
Conclusions: Our results argue for clonal or oligoclonal TCRs to crude peanut and indicate that changes in the TCRBV11 subpopulation are restricted to peanut-allergic subjects after stimulation with crude peanut allergen.
peanut allergy, t-cell clonality, tcr-v? families
0091-6749
1089-1094
Bakakos, Petros
ece13698-4851-4525-94e0-a2a5cf86d100
Smith, John L.
221ab2fa-1f8d-4e15-9eeb-9dd77acfcf4d
Warner, John O.
50630e99-8486-4859-ade3-cd2c79c5a153
Vance, Gillian
365f5f1c-d391-457d-b323-33afad372e14
Moss, Christine T.
5afbc34f-f1a0-4925-9bda-50209f5decc3
Hodges, Elizabeth
d264e1f4-c70f-4b4a-8f37-424be76728f6
Lanham, Stuart
8f98af44-c970-4b4f-b57e-845d0c5775dc
Howell, W.Martin
5ea555ef-ffee-48ff-93a0-0030167e6b29
Bakakos, Petros
ece13698-4851-4525-94e0-a2a5cf86d100
Smith, John L.
221ab2fa-1f8d-4e15-9eeb-9dd77acfcf4d
Warner, John O.
50630e99-8486-4859-ade3-cd2c79c5a153
Vance, Gillian
365f5f1c-d391-457d-b323-33afad372e14
Moss, Christine T.
5afbc34f-f1a0-4925-9bda-50209f5decc3
Hodges, Elizabeth
d264e1f4-c70f-4b4a-8f37-424be76728f6
Lanham, Stuart
8f98af44-c970-4b4f-b57e-845d0c5775dc
Howell, W.Martin
5ea555ef-ffee-48ff-93a0-0030167e6b29

Bakakos, Petros, Smith, John L., Warner, John O., Vance, Gillian, Moss, Christine T., Hodges, Elizabeth, Lanham, Stuart and Howell, W.Martin (2001) Modification of T-cell receptor Vβ repertoire in response to allergen stimulation in peanut allergy. Journal of Allergy and Clinical Immunology, 107 (6), 1089-1094. (doi:10.1067/mai.2001.114651).

Record type: Article

Abstract

Background: Peanut is one of the most common foods causing allergic reactions and is the most common cause of fatal and near-fatal food-related anaphylaxis. Little is known of the immunologic mechanisms that underlie peanut allergy. Objectives: In this study we examined clonality of the T-cell response (TCR) to peanut in MHC class II identical, peanut allergy–discordant sibling pairs.
Methods: Four sibling pairs were investigated. The TCR repertoire was analyzed before and after in vitro stimulation of PBMCs with crude peanut or PHA, as control for general/nonspecific reactivity. Eighteen TCR-Vβ families were examined by flow cytometry. Where significant differences in incidence of particular TCR-Vβ families were observed, PCR familyspecific cDNA amplification and gene scanning were performed.
Results: After stimulation with peanut, no selective expansion of any TCR-Vβ subpopulation was observed with flow cytometry, in either the peanut-allergic or nonallergic siblings, with the exception of 1 peanut-allergic subject who demonstrated a significant increase of TCR-Vβ11+ cells (0.3%-5.9% of the total CD3+ cells). However, gene scanning revealed predominant single-size PCR products for TCRBV11 in all peanut-allergic subjects after peanut stimulation. TCRBV11 polyclonality was observed in allergic and nonallergic subjects before peanut stimulation and in nonallergic subjects after peanut stimulation. In comparison, all subjects, before and after stimulation with peanut, showed polyclonality for TCRBV2.
Conclusions: Our results argue for clonal or oligoclonal TCRs to crude peanut and indicate that changes in the TCRBV11 subpopulation are restricted to peanut-allergic subjects after stimulation with crude peanut allergen.

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More information

Published date: 2001
Additional Information: Original Articles: Food and Drug Reactions and Anaphylaxis
Keywords: peanut allergy, t-cell clonality, tcr-v? families

Identifiers

Local EPrints ID: 26922
URI: http://eprints.soton.ac.uk/id/eprint/26922
ISSN: 0091-6749
PURE UUID: 8b5702ec-76b3-4091-9002-c788920ad633

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Date deposited: 26 Apr 2006
Last modified: 15 Mar 2024 07:14

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Contributors

Author: Petros Bakakos
Author: John L. Smith
Author: John O. Warner
Author: Gillian Vance
Author: Christine T. Moss
Author: Elizabeth Hodges
Author: Stuart Lanham
Author: W.Martin Howell

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