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TCR usage and cytokine expression in peripheral blood and BAL T cells

TCR usage and cytokine expression in peripheral blood and BAL T cells
TCR usage and cytokine expression in peripheral blood and BAL T cells
T cells are thought to play an important regulatory role in atopic asthma. We hypothesized that human blood and BAL T cell subsets bearing various TCR-V? genes might show selective differences in their cytokine profile. Peripheral blood (PB) and bronchoalveolar lavage (BAL) T cells from seven atopic asthmatic and six non-atopic non-asthmatic subjects were stimulated with PMA and ionomycin in the presence of monensin and analysed for TCR-V? expression and production of cytokines at the single cell level. The percentage of IFN-?- and IL-2-producing BAL T cells was elevated compared with PB T cells from both the asthmatic subjects and the non-atopic, non-asthmatic controls. A small percentage of PB and BAL T cells produced IL-4 and IL-5, in asthmatic and normal subjects. In peripheral blood, the percentage of T cells expressing each cytokine was similar in the various TCR-V? subsets and in total CD3+ T cells in all normal and six of seven asthmatic subjects. However, there was a substantial degree of heterogeneity in the cytokine profile of BAL TCR-V? subsets compared with the total CD3+ T cells. This was more obvious in the asthmatic subjects with a reduction in the percentage of IFN-?- and IL-2-expressing T cells (five of seven asthmatic subjects) and an increase in the percentage of IL-4- and IL-5-expressing T cells (two of seven asthmatic subjects). These data confirm previous findings of an elevated proportion of IFN-?- and IL-2-producing BAL T cells while only a small proportion of PB and BAL T cells produce IL-4 and IL-5. Moreover, subsets of BAL T cells, defined by their TCR-V? usage, may differ in their cytokine profile compared with the total CD3+ T cells, implying that T cells expressing different V? elements may play different roles in regulating the airway inflammation in asthma.
asthma, cytokines, flow cytometry, tcr
0009-9104
295-301
Bakakos, P.
3570861d-e41b-4ec5-91e2-9fec071e696c
Pickard, C.
e21117b3-6345-4d09-a876-ac9965ec4d6a
Smith, J.L.
f9291742-f9d3-4e27-bfe6-94d41e816fa8
Frew, A.J.
c00e9630-a5f0-44b3-add0-44b68836bbcb
Bakakos, P.
3570861d-e41b-4ec5-91e2-9fec071e696c
Pickard, C.
e21117b3-6345-4d09-a876-ac9965ec4d6a
Smith, J.L.
f9291742-f9d3-4e27-bfe6-94d41e816fa8
Frew, A.J.
c00e9630-a5f0-44b3-add0-44b68836bbcb

Bakakos, P., Pickard, C., Smith, J.L. and Frew, A.J. (2002) TCR usage and cytokine expression in peripheral blood and BAL T cells. Clinical and Experimental Immunology, 128 (2), 295-301. (doi:10.1046/j.1365-2249.2002.01847.x).

Record type: Article

Abstract

T cells are thought to play an important regulatory role in atopic asthma. We hypothesized that human blood and BAL T cell subsets bearing various TCR-V? genes might show selective differences in their cytokine profile. Peripheral blood (PB) and bronchoalveolar lavage (BAL) T cells from seven atopic asthmatic and six non-atopic non-asthmatic subjects were stimulated with PMA and ionomycin in the presence of monensin and analysed for TCR-V? expression and production of cytokines at the single cell level. The percentage of IFN-?- and IL-2-producing BAL T cells was elevated compared with PB T cells from both the asthmatic subjects and the non-atopic, non-asthmatic controls. A small percentage of PB and BAL T cells produced IL-4 and IL-5, in asthmatic and normal subjects. In peripheral blood, the percentage of T cells expressing each cytokine was similar in the various TCR-V? subsets and in total CD3+ T cells in all normal and six of seven asthmatic subjects. However, there was a substantial degree of heterogeneity in the cytokine profile of BAL TCR-V? subsets compared with the total CD3+ T cells. This was more obvious in the asthmatic subjects with a reduction in the percentage of IFN-?- and IL-2-expressing T cells (five of seven asthmatic subjects) and an increase in the percentage of IL-4- and IL-5-expressing T cells (two of seven asthmatic subjects). These data confirm previous findings of an elevated proportion of IFN-?- and IL-2-producing BAL T cells while only a small proportion of PB and BAL T cells produce IL-4 and IL-5. Moreover, subsets of BAL T cells, defined by their TCR-V? usage, may differ in their cytokine profile compared with the total CD3+ T cells, implying that T cells expressing different V? elements may play different roles in regulating the airway inflammation in asthma.

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More information

Published date: 2002
Keywords: asthma, cytokines, flow cytometry, tcr

Identifiers

Local EPrints ID: 26924
URI: http://eprints.soton.ac.uk/id/eprint/26924
ISSN: 0009-9104
PURE UUID: 3e242018-9742-44bf-ba23-4ac3749ebbb6

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Date deposited: 25 Apr 2006
Last modified: 15 Jul 2019 19:13

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