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Apoptosis and proliferation of acinar and islet cells in chronic pancreatitis: evidence for differential cell loss mediating preservation of islet function

Apoptosis and proliferation of acinar and islet cells in chronic pancreatitis: evidence for differential cell loss mediating preservation of islet function
Apoptosis and proliferation of acinar and islet cells in chronic pancreatitis: evidence for differential cell loss mediating preservation of islet function
BACKGROUND: Chronic pancreatitis is characterised clinically by early exocrine insufficiency, with diabetes mellitus occurring as a late phenomenon. This is mirrored pathologically by extensive acinar cell destruction and islet preservation. The mechanisms underlying this differential rate of cellular destruction are unknown.
AIMS: To test the hypothesis that acinar loss and islet preservation in chronic pancreatitis occurs due to differential epithelial kinetics and investigate the role of inflammatory cells and cell cycle associated molecules.
METHODS: Archival tissue from six chronic pancreatitis cases was compared with six normal controls using TUNEL and immunohistochemistry for CD3, CD20, CD68, MIB-1, Bcl-2, Bax, Fas, Fas ligand, retinoblastoma protein (Rb), and tissue inhibitor of metalloproteinases 1 (TIMP-1) and 2 (TIMP-2).
RESULTS: The acinar cell apoptotic index (AI) and proliferation index were higher in chronic pancreatitis than controls. T lymphocytes diffusely infiltrated fibrous bands and acini but rarely islets. Acinar Bcl-2 expression exceeded islet expression in chronic pancreatitis and controls while Bax was strongly expressed by a subset of islet cells and weakly by centroacinar cells. Islet Fas and Fas ligand expression exceeded acinar expression in chronic pancreatitis and controls. Acinar Rb expression was higher in chronic pancreatitis than in controls. Islets in chronic pancreatitis and controls showed intense TIMP-1 and TIMP-2 expression.
CONCLUSION: Apoptosis plays a significant role in acinar loss in chronic pancreatitis. Acinar Bcl-2 and islet Bax expression indicates complex AI control. Increased acinar Rb expression in chronic pancreatitis may differentially promote acinar loss. Fas ligand expression may be restricted to islet cell membranes through TIMP-1 expression and inhibit islet damage by promoting apoptosis of cytotoxic T lymphocytes.
0017-5749
542-548
Bateman, A.C.
4e97a5ca-662c-451d-bdb3-33f35420ceed
Turner, S.M.
42f19397-8e9f-435d-a348-2cc1639b5eb4
Thomas, K.S.
3eb8b4f5-2076-4edc-a7f5-30787773e1bf
McCrudden, P.R.
e6839e7b-e28c-4c31-b5e6-273c3352d5ae
Fine, D.R.
ce3d6a77-040e-4aec-a8f5-4c4c22431605
Johnson, P.A.
f78f4500-4972-48ff-b6b4-6630f925712a
Johnson, C.D.
e50aa9cd-8c61-4fe3-a0b3-f51cc3a6c74a
Iredale, J.P.
5c8dbd67-954f-4a50-b084-af1d253277cb
Bateman, A.C.
4e97a5ca-662c-451d-bdb3-33f35420ceed
Turner, S.M.
42f19397-8e9f-435d-a348-2cc1639b5eb4
Thomas, K.S.
3eb8b4f5-2076-4edc-a7f5-30787773e1bf
McCrudden, P.R.
e6839e7b-e28c-4c31-b5e6-273c3352d5ae
Fine, D.R.
ce3d6a77-040e-4aec-a8f5-4c4c22431605
Johnson, P.A.
f78f4500-4972-48ff-b6b4-6630f925712a
Johnson, C.D.
e50aa9cd-8c61-4fe3-a0b3-f51cc3a6c74a
Iredale, J.P.
5c8dbd67-954f-4a50-b084-af1d253277cb

Bateman, A.C., Turner, S.M., Thomas, K.S., McCrudden, P.R., Fine, D.R., Johnson, P.A., Johnson, C.D. and Iredale, J.P. (2002) Apoptosis and proliferation of acinar and islet cells in chronic pancreatitis: evidence for differential cell loss mediating preservation of islet function. Gut, 50 (4), 542-548. (doi:10.1136/gut.50.4.542).

Record type: Article

Abstract

BACKGROUND: Chronic pancreatitis is characterised clinically by early exocrine insufficiency, with diabetes mellitus occurring as a late phenomenon. This is mirrored pathologically by extensive acinar cell destruction and islet preservation. The mechanisms underlying this differential rate of cellular destruction are unknown.
AIMS: To test the hypothesis that acinar loss and islet preservation in chronic pancreatitis occurs due to differential epithelial kinetics and investigate the role of inflammatory cells and cell cycle associated molecules.
METHODS: Archival tissue from six chronic pancreatitis cases was compared with six normal controls using TUNEL and immunohistochemistry for CD3, CD20, CD68, MIB-1, Bcl-2, Bax, Fas, Fas ligand, retinoblastoma protein (Rb), and tissue inhibitor of metalloproteinases 1 (TIMP-1) and 2 (TIMP-2).
RESULTS: The acinar cell apoptotic index (AI) and proliferation index were higher in chronic pancreatitis than controls. T lymphocytes diffusely infiltrated fibrous bands and acini but rarely islets. Acinar Bcl-2 expression exceeded islet expression in chronic pancreatitis and controls while Bax was strongly expressed by a subset of islet cells and weakly by centroacinar cells. Islet Fas and Fas ligand expression exceeded acinar expression in chronic pancreatitis and controls. Acinar Rb expression was higher in chronic pancreatitis than in controls. Islets in chronic pancreatitis and controls showed intense TIMP-1 and TIMP-2 expression.
CONCLUSION: Apoptosis plays a significant role in acinar loss in chronic pancreatitis. Acinar Bcl-2 and islet Bax expression indicates complex AI control. Increased acinar Rb expression in chronic pancreatitis may differentially promote acinar loss. Fas ligand expression may be restricted to islet cell membranes through TIMP-1 expression and inhibit islet damage by promoting apoptosis of cytotoxic T lymphocytes.

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Published date: 2002

Identifiers

Local EPrints ID: 26929
URI: http://eprints.soton.ac.uk/id/eprint/26929
ISSN: 0017-5749
PURE UUID: fff07ef7-6d36-47fb-bc68-5a63de66fba8

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Date deposited: 25 Apr 2006
Last modified: 20 Nov 2019 17:31

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