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Selective alterations in mast cell subsets and eosinophil infiltration in two complementary types of intestinal inflammation: ascariasis and Crohn's disease

Selective alterations in mast cell subsets and eosinophil infiltration in two complementary types of intestinal inflammation: ascariasis and Crohn's disease
Selective alterations in mast cell subsets and eosinophil infiltration in two complementary types of intestinal inflammation: ascariasis and Crohn's disease
Objective: Numbers of mast cells (MCs) of different subpopulations and the extent of eosinophil infiltration were compared in Crohn's disease and ascariasis. These two types of intestinal inflammation are complementary with regard to T cell response (TH1 versus TH2), prevalence and environmental factors.
Methods: Histochemical, immunohistochemical and ultrastructural tools were applied to biopsies of morphologically uninvolved colon, ileum and duodenum from Crohn's and ascariasis patients, as well as resection margins and tissues from an experimental porcine ascariasis model. MC subsets were defined by their dye-binding properties, and their chymase content was analysed using biochemical tools.
Results: The TH2 (IgE-mediated) response in ascariasis was characterised by a dramatic increase in mucosal- type MCs (MMCs) and eosinophils in both the mucosa and the deeper layers of the intestinal wall and a simultaneous decrease of connective tissue-type MCs (CTMCs). Uninvolved intestine of Crohn's patients showed moderate proliferation of CTMCs in the deeper layers of the intestinal wall, but a significant decrease of the MMCs, associated with moderate eosinophilia in all layers of the gut. Similar changes were present in the uninvolved duodenum of Crohn's patients. Comparable amounts of chymase could be extracted from mucosal and submucosal duodenum, with similar proportions of its two principal isoforms in each.
Conclusions: Our results indicate that T cell responses (TH1 or TH2) are associated with different MC subsets in intestinal inflammation. Changes remote from the focus of inflammation point to the systemic nature of the different MC responses.
ascariasis, crohn's disease, th1, th2, mast cell subsets, chymase, heparin, eosinophils
303-313
Beil, Waltraud J.
10098842-abc8-48f5-987c-decdc45a8f51
McEuen, Alan R.
e2054594-c27d-4d45-86ed-c899dc401c3a
Schulz, Matthias
ea9a8118-d755-4b6d-993a-6eb4ad4e4552
Wefelmeyer, Udo
03356432-a28e-4ea7-9272-e14c696b9c04
Kraml, Georg
1b1afbe9-c2e6-4320-8651-2ec2a842863e
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Jensen-Jarolim, Erika
c99d1329-6068-40ad-affc-06033efd354e
Pabst, Reinhard
237fc2d2-f951-47f3-8314-3076912494f2
Pammer, Johannes
92d26a8b-be3e-4b8c-ad58-8341c1e7b54e
Beil, Waltraud J.
10098842-abc8-48f5-987c-decdc45a8f51
McEuen, Alan R.
e2054594-c27d-4d45-86ed-c899dc401c3a
Schulz, Matthias
ea9a8118-d755-4b6d-993a-6eb4ad4e4552
Wefelmeyer, Udo
03356432-a28e-4ea7-9272-e14c696b9c04
Kraml, Georg
1b1afbe9-c2e6-4320-8651-2ec2a842863e
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Jensen-Jarolim, Erika
c99d1329-6068-40ad-affc-06033efd354e
Pabst, Reinhard
237fc2d2-f951-47f3-8314-3076912494f2
Pammer, Johannes
92d26a8b-be3e-4b8c-ad58-8341c1e7b54e

Beil, Waltraud J., McEuen, Alan R., Schulz, Matthias, Wefelmeyer, Udo, Kraml, Georg, Walls, Andrew F., Jensen-Jarolim, Erika, Pabst, Reinhard and Pammer, Johannes (2002) Selective alterations in mast cell subsets and eosinophil infiltration in two complementary types of intestinal inflammation: ascariasis and Crohn's disease. Pathobiology, 70 (6), 303-313. (doi:10.1159/000071270).

Record type: Article

Abstract

Objective: Numbers of mast cells (MCs) of different subpopulations and the extent of eosinophil infiltration were compared in Crohn's disease and ascariasis. These two types of intestinal inflammation are complementary with regard to T cell response (TH1 versus TH2), prevalence and environmental factors.
Methods: Histochemical, immunohistochemical and ultrastructural tools were applied to biopsies of morphologically uninvolved colon, ileum and duodenum from Crohn's and ascariasis patients, as well as resection margins and tissues from an experimental porcine ascariasis model. MC subsets were defined by their dye-binding properties, and their chymase content was analysed using biochemical tools.
Results: The TH2 (IgE-mediated) response in ascariasis was characterised by a dramatic increase in mucosal- type MCs (MMCs) and eosinophils in both the mucosa and the deeper layers of the intestinal wall and a simultaneous decrease of connective tissue-type MCs (CTMCs). Uninvolved intestine of Crohn's patients showed moderate proliferation of CTMCs in the deeper layers of the intestinal wall, but a significant decrease of the MMCs, associated with moderate eosinophilia in all layers of the gut. Similar changes were present in the uninvolved duodenum of Crohn's patients. Comparable amounts of chymase could be extracted from mucosal and submucosal duodenum, with similar proportions of its two principal isoforms in each.
Conclusions: Our results indicate that T cell responses (TH1 or TH2) are associated with different MC subsets in intestinal inflammation. Changes remote from the focus of inflammation point to the systemic nature of the different MC responses.

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More information

Published date: 2002
Keywords: ascariasis, crohn's disease, th1, th2, mast cell subsets, chymase, heparin, eosinophils

Identifiers

Local EPrints ID: 26934
URI: http://eprints.soton.ac.uk/id/eprint/26934
PURE UUID: 3ffa12e2-7fce-4f59-a2d6-3c3a9ce5337d
ORCID for Andrew F. Walls: ORCID iD orcid.org/0000-0003-4803-4595

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Date deposited: 25 Apr 2006
Last modified: 18 Feb 2021 16:36

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Contributors

Author: Waltraud J. Beil
Author: Alan R. McEuen
Author: Matthias Schulz
Author: Udo Wefelmeyer
Author: Georg Kraml
Author: Andrew F. Walls ORCID iD
Author: Erika Jensen-Jarolim
Author: Reinhard Pabst
Author: Johannes Pammer

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