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Tryptase-stimulated human airway smooth muscle cells induce cytokine synthesis and mast cell chemotaxis

Tryptase-stimulated human airway smooth muscle cells induce cytokine synthesis and mast cell chemotaxis
Tryptase-stimulated human airway smooth muscle cells induce cytokine synthesis and mast cell chemotaxis
Asthmatic patients have higher numbers of mast cells in the smooth muscle layer of airways than normal subjects. Human airway smooth muscle cells (HASMCs) are a source of various cytokines including transforming growth factor ?1 (TGF-?1), which is chemotactic for mast cells. We have thus examined the potential for interaction between HASMCs and mast cells and have investigated, in particular, the hypothesis that after stimulation, HASMCs can induce mast cell chemotaxis through the production of cytokines. Supernatants of HASMCs treated with the major mast cell product tryptase had increased chemotactic activity for the HMC-1 mast cell line. The effect depended on an intact catalytic site for tryptase and could be induced by a peptide agonist for protease activated receptor 2. Chemotactic activity was related to the synthesis of TGF-?1 by HASMCs and, to a lesser extent, to stem cell factor. The number of mast cells within the smooth muscle layer of asthmatic patients was closely related to TGF-?1 expression by smooth muscle. HASMCs may thus be able to stimulate the accumulation of mast cells, and these cells may, in turn, stimulate the secretion of chemotactic factors by HASMCs.
asthma, protease-activated receptor, transforming growth factor ?1, stem cell factor
0892-6638
2139-2141
Berger, Patrick
ad4f2bc1-57f7-4af1-916f-b00415a56c1a
Girodet, Pierre-Olivier
63f8eaff-06dd-443b-8e40-dc23d4a68ae6
Begueret, Hugues
92ca40cb-13c6-47b8-88b2-728a35393280
Ousova, Olga
f7e00385-35db-4494-ae29-9dfb2052ac12
Perng, Diahn-Warng
0718eeb7-4ac4-4050-b69b-4924e91d1f8b
Marthan, Roger
18a5680f-fb7a-4980-a787-342cbc93c8b2
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
de Lara, J. Manuel Tunon
d45379bb-a5eb-40ae-af65-9337ccffdeba
Berger, Patrick
ad4f2bc1-57f7-4af1-916f-b00415a56c1a
Girodet, Pierre-Olivier
63f8eaff-06dd-443b-8e40-dc23d4a68ae6
Begueret, Hugues
92ca40cb-13c6-47b8-88b2-728a35393280
Ousova, Olga
f7e00385-35db-4494-ae29-9dfb2052ac12
Perng, Diahn-Warng
0718eeb7-4ac4-4050-b69b-4924e91d1f8b
Marthan, Roger
18a5680f-fb7a-4980-a787-342cbc93c8b2
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
de Lara, J. Manuel Tunon
d45379bb-a5eb-40ae-af65-9337ccffdeba

Berger, Patrick, Girodet, Pierre-Olivier, Begueret, Hugues, Ousova, Olga, Perng, Diahn-Warng, Marthan, Roger, Walls, Andrew F. and de Lara, J. Manuel Tunon (2003) Tryptase-stimulated human airway smooth muscle cells induce cytokine synthesis and mast cell chemotaxis. The FASEB Journal, 17 (14), 2139-2141. (doi:10.1096/fj.03-0041fje).

Record type: Article

Abstract

Asthmatic patients have higher numbers of mast cells in the smooth muscle layer of airways than normal subjects. Human airway smooth muscle cells (HASMCs) are a source of various cytokines including transforming growth factor ?1 (TGF-?1), which is chemotactic for mast cells. We have thus examined the potential for interaction between HASMCs and mast cells and have investigated, in particular, the hypothesis that after stimulation, HASMCs can induce mast cell chemotaxis through the production of cytokines. Supernatants of HASMCs treated with the major mast cell product tryptase had increased chemotactic activity for the HMC-1 mast cell line. The effect depended on an intact catalytic site for tryptase and could be induced by a peptide agonist for protease activated receptor 2. Chemotactic activity was related to the synthesis of TGF-?1 by HASMCs and, to a lesser extent, to stem cell factor. The number of mast cells within the smooth muscle layer of asthmatic patients was closely related to TGF-?1 expression by smooth muscle. HASMCs may thus be able to stimulate the accumulation of mast cells, and these cells may, in turn, stimulate the secretion of chemotactic factors by HASMCs.

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Published date: 2003
Keywords: asthma, protease-activated receptor, transforming growth factor ?1, stem cell factor
Organisations: Infection Inflammation & Immunity

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Local EPrints ID: 26938
URI: https://eprints.soton.ac.uk/id/eprint/26938
ISSN: 0892-6638
PURE UUID: 25d18745-7a67-465e-b69a-1a4813ee46ce

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Date deposited: 25 Apr 2006
Last modified: 15 Jul 2019 19:13

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Contributors

Author: Patrick Berger
Author: Pierre-Olivier Girodet
Author: Hugues Begueret
Author: Olga Ousova
Author: Diahn-Warng Perng
Author: Roger Marthan
Author: Andrew F. Walls
Author: J. Manuel Tunon de Lara

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