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Ozone-induced bronchial epithelial cytokine expression differs between healthy and asthmatic subjects

Ozone-induced bronchial epithelial cytokine expression differs between healthy and asthmatic subjects
Ozone-induced bronchial epithelial cytokine expression differs between healthy and asthmatic subjects
Background Ozone (O3) is a common air pollutant associated with adverse health effects. Asthmatics have been suggested to be a particularly sensitive group.

Objective This study evaluated whether bronchial epithelial cytokine expression would differ between healthy and allergic asthmatics after ozone exposure, representing an explanatory model for differences in susceptibility.

Methods Healthy and mild allergic asthmatic subjects (using only inhaled β2‐agonists prn) were exposed for 2h in blinded and randomized sequence to 0.2ppm of O3 and filtered air. Bronchoscopy with bronchial mucosal biopsies was performed 6 h after exposure. Biopsies were embedded in GMA and stained with mAbs for epithelial expression of IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, TNF‐α, GRO‐α, granulocyte–macrophage colony‐stimulating factor (GM–CSF), fractalkine and ENA‐78.

Results When comparing the two groups at baseline, the asthmatic subjects showed a significantly higher expression of IL‐4 and IL‐5. After O3 exposure the epithelial expression of IL‐5, GM–CSF, ENA‐78 and IL‐8 increased significantly in asthmatics, as compared to healthy subjects.

Conclusion The present study confirms a difference in epithelial cytokine expression between mild atopic asthmatics and healthy controls, as well as a differential epithelial cytokine response to O3. This O3‐induced upregulation of T helper type 2 (Th2)‐related cytokines and neutrophil chemoattractants shown in the asthmatic group may contribute to a subsequent worsening of the airway inflammation, and help to explain their differential sensitivity to O3 pollution episodes.
0954-7894
777-782
Bosson, J.
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Stenfors, N.
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Bucht, A.
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Helleday, R.
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Pourazar, J.
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Holgate, S.T.
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Kelly, F.J.
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Sandström, T.
d3d026b6-08f7-4c4b-acc4-9c621390af69
Wilson, S.
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Frew, A.J.
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Blomberg, A.
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Bosson, J.
16151692-08c4-42a9-8dcf-4f3feb3cafb1
Stenfors, N.
f4570515-de95-436c-ae7a-6cef4b45b3d3
Bucht, A.
f3c19715-8168-4825-9b7e-36d6c23baedb
Helleday, R.
8fd5b0ef-8a37-4260-ba04-8b0b707b4fcc
Pourazar, J.
8cf3c9a6-c253-4602-86a4-331209a703a7
Holgate, S.T.
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Kelly, F.J.
0662f749-2cc5-465a-a4aa-2a6ff64146ac
Sandström, T.
d3d026b6-08f7-4c4b-acc4-9c621390af69
Wilson, S.
21c6875d-6870-441b-ae7a-603562a646b8
Frew, A.J.
c00e9630-a5f0-44b3-add0-44b68836bbcb
Blomberg, A.
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Bosson, J., Stenfors, N., Bucht, A., Helleday, R., Pourazar, J., Holgate, S.T., Kelly, F.J., Sandström, T., Wilson, S., Frew, A.J. and Blomberg, A. (2003) Ozone-induced bronchial epithelial cytokine expression differs between healthy and asthmatic subjects. Clinical & Experimental Allergy, 33 (6), 777-782. (doi:10.1046/j.1365-2222.2003.01662.x).

Record type: Article

Abstract

Background Ozone (O3) is a common air pollutant associated with adverse health effects. Asthmatics have been suggested to be a particularly sensitive group.

Objective This study evaluated whether bronchial epithelial cytokine expression would differ between healthy and allergic asthmatics after ozone exposure, representing an explanatory model for differences in susceptibility.

Methods Healthy and mild allergic asthmatic subjects (using only inhaled β2‐agonists prn) were exposed for 2h in blinded and randomized sequence to 0.2ppm of O3 and filtered air. Bronchoscopy with bronchial mucosal biopsies was performed 6 h after exposure. Biopsies were embedded in GMA and stained with mAbs for epithelial expression of IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, TNF‐α, GRO‐α, granulocyte–macrophage colony‐stimulating factor (GM–CSF), fractalkine and ENA‐78.

Results When comparing the two groups at baseline, the asthmatic subjects showed a significantly higher expression of IL‐4 and IL‐5. After O3 exposure the epithelial expression of IL‐5, GM–CSF, ENA‐78 and IL‐8 increased significantly in asthmatics, as compared to healthy subjects.

Conclusion The present study confirms a difference in epithelial cytokine expression between mild atopic asthmatics and healthy controls, as well as a differential epithelial cytokine response to O3. This O3‐induced upregulation of T helper type 2 (Th2)‐related cytokines and neutrophil chemoattractants shown in the asthmatic group may contribute to a subsequent worsening of the airway inflammation, and help to explain their differential sensitivity to O3 pollution episodes.

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Published date: 2003

Identifiers

Local EPrints ID: 26946
URI: http://eprints.soton.ac.uk/id/eprint/26946
ISSN: 0954-7894
PURE UUID: 735b9b9e-8820-42fd-b8e7-f7f1621fe57e
ORCID for S. Wilson: ORCID iD orcid.org/0000-0003-1305-8271

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Date deposited: 25 Apr 2006
Last modified: 08 Jan 2022 02:37

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Contributors

Author: J. Bosson
Author: N. Stenfors
Author: A. Bucht
Author: R. Helleday
Author: J. Pourazar
Author: S.T. Holgate
Author: F.J. Kelly
Author: T. Sandström
Author: S. Wilson ORCID iD
Author: A.J. Frew
Author: A. Blomberg

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