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Recovery of nitric oxide from acetylcholine-mediated vasodilatation in human skin in vivo

Recovery of nitric oxide from acetylcholine-mediated vasodilatation in human skin in vivo
Recovery of nitric oxide from acetylcholine-mediated vasodilatation in human skin in vivo
Objectives: To investigate the relative contribution of nitric oxide (NO) to the vascular and neural mechanisms underlying the ACh-induced vasodilatation in human skin. Methods: ACh was delivered to the skin of the forearm of 28 healthy volunteers using intradermal microdialysis. Subsequent changes in tissue levels of NO and histamine were measured in the dialysate outflow and the associated changes in skin blood flux followed with the use of scanning laser Doppler imaging. Results: ACh caused a dose-dependent increase in skin blood flux measured directly above the probe, associated with a twofold increase in dialysate NO. L-NAME (5 mM) delivered simultaneously via the dialysis probe totally blocked the increase in dialysate NO but only partially attenuated (?30%) the ACh-induced increase in blood flux. At concentrations?6.25 mM, ACh also induced a widespread flare response, up to 40 mm in width, accompanied by the sensation of itch. The flare was not blocked by L-NAME or the H1 receptor antagonist levocetirizine, but was reduced by C-fiber blockade. Dialysate histamine levels remained unchanged at all times. Conclusions: These experiments offer further insight into the use of dialysis as an experimental technique in the skin. They provide direct evidence that the skin microvascular response to ACh is only partially mediated by NO. Further they suggest that ACh at higher concentrations can induce an axon-reflex-mediated response that is independent of NO release at the site of dermal provocation or of local histamine release.
microdialysis, microvasculature, nitric oxide, skin
1073-9688
249-259
Boutsiouki, Paraskevi
7bcd1b5c-184a-4624-9419-0c2c78faa0df
Georgiou, Sophia
db912423-82c9-43ba-a710-ec1ba2e7d0ea
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494
Boutsiouki, Paraskevi
7bcd1b5c-184a-4624-9419-0c2c78faa0df
Georgiou, Sophia
db912423-82c9-43ba-a710-ec1ba2e7d0ea
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494

Boutsiouki, Paraskevi, Georgiou, Sophia and Clough, Geraldine F. (2004) Recovery of nitric oxide from acetylcholine-mediated vasodilatation in human skin in vivo. Microcirculation, 11 (3), 249-259. (doi:10.1080/10739680490425958).

Record type: Article

Abstract

Objectives: To investigate the relative contribution of nitric oxide (NO) to the vascular and neural mechanisms underlying the ACh-induced vasodilatation in human skin. Methods: ACh was delivered to the skin of the forearm of 28 healthy volunteers using intradermal microdialysis. Subsequent changes in tissue levels of NO and histamine were measured in the dialysate outflow and the associated changes in skin blood flux followed with the use of scanning laser Doppler imaging. Results: ACh caused a dose-dependent increase in skin blood flux measured directly above the probe, associated with a twofold increase in dialysate NO. L-NAME (5 mM) delivered simultaneously via the dialysis probe totally blocked the increase in dialysate NO but only partially attenuated (?30%) the ACh-induced increase in blood flux. At concentrations?6.25 mM, ACh also induced a widespread flare response, up to 40 mm in width, accompanied by the sensation of itch. The flare was not blocked by L-NAME or the H1 receptor antagonist levocetirizine, but was reduced by C-fiber blockade. Dialysate histamine levels remained unchanged at all times. Conclusions: These experiments offer further insight into the use of dialysis as an experimental technique in the skin. They provide direct evidence that the skin microvascular response to ACh is only partially mediated by NO. Further they suggest that ACh at higher concentrations can induce an axon-reflex-mediated response that is independent of NO release at the site of dermal provocation or of local histamine release.

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More information

Published date: 2004
Keywords: microdialysis, microvasculature, nitric oxide, skin

Identifiers

Local EPrints ID: 26954
URI: https://eprints.soton.ac.uk/id/eprint/26954
ISSN: 1073-9688
PURE UUID: 5c41acce-e18e-4a85-b1cb-e4804bfc9552
ORCID for Geraldine F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

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Date deposited: 25 Apr 2006
Last modified: 06 Jun 2018 13:02

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