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Segmental bronchoprovocation in allergic rhinitis patients affects mast cell and basophil numbers in nasal and bronchial mucosa

Segmental bronchoprovocation in allergic rhinitis patients affects mast cell and basophil numbers in nasal and bronchial mucosa
Segmental bronchoprovocation in allergic rhinitis patients affects mast cell and basophil numbers in nasal and bronchial mucosa
Mast cells and basophils are cells that play an important role in the initiation and control of allergic inflammation in asthma and rhinitis. This study was undertaken to determine the presence and dynamics of mast cells and basophils in the nasal and bronchial mucosa of allergic rhinitis patients after segmental bronchial provocation (SBP). Eight nonasthmatic, grass pollen-allergic rhinitis patients and eight healthy controls were included. Bronchial and nasal biopsies, as well as blood samples, were taken before (T0) and 24 h (T24) after SBP. Immunohistochemical staining was performed for mast cells (tryptase and chymase; phenotypes MCT, MCTC, MCC) and basophils (BB1). In the bronchial mucosa, the number of BB1+ cells increased significantly (p < 0.05) in allergic rhinitis patients after SBP. In the nasal mucosa, the numbers of MCC and MCTC cells decreased significantly, whereas the numbers of [BB1+] cells increased significantly in allergic rhinitis patients after SBP (p < 0.05). In blood, the number of basophils decreased (p < 0.05) and the level of interleukin (IL)-5 increased (p < 0.05) in atopic patients after SBP. No significant changes could be observed in healthy controls. This study shows that SBP in nonasthmatic allergic rhinitis patients reduces numbers of mast cells in the nose as a result of enhanced degranulation. At the same time, there is evidence for an influx of basophils from the blood into the nasal and bronchial mucosae.
bronchoprovocation, asthma, rhinitis, biopsy, inflammation
1073-449X
858-865
Braunstahl, Gert-Jan
89fdced3-eee6-462e-8d73-50004ce07cda
Overbeek, Shelley E.
aae9517b-2828-4cbe-8c39-a23317007557
Fokkens, Wytske J.
717d2f9b-ba83-414f-8c1e-2998e1fc4cba
Kleinjan, Alex
cacfb29f-7cf4-45f4-947c-0c8c42bf52ca
McEuen, Alan R.
e2054594-c27d-4d45-86ed-c899dc401c3a
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Hoogsteden, Henk C.
5d296746-4a55-4e7e-824a-4dc61a3a2dcd
Prins, Jan-Bas
f8ce9f75-dc23-456e-828e-b8dfe3ea970f
Braunstahl, Gert-Jan
89fdced3-eee6-462e-8d73-50004ce07cda
Overbeek, Shelley E.
aae9517b-2828-4cbe-8c39-a23317007557
Fokkens, Wytske J.
717d2f9b-ba83-414f-8c1e-2998e1fc4cba
Kleinjan, Alex
cacfb29f-7cf4-45f4-947c-0c8c42bf52ca
McEuen, Alan R.
e2054594-c27d-4d45-86ed-c899dc401c3a
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Hoogsteden, Henk C.
5d296746-4a55-4e7e-824a-4dc61a3a2dcd
Prins, Jan-Bas
f8ce9f75-dc23-456e-828e-b8dfe3ea970f

Braunstahl, Gert-Jan, Overbeek, Shelley E., Fokkens, Wytske J., Kleinjan, Alex, McEuen, Alan R., Walls, Andrew F., Hoogsteden, Henk C. and Prins, Jan-Bas (2001) Segmental bronchoprovocation in allergic rhinitis patients affects mast cell and basophil numbers in nasal and bronchial mucosa. American Journal of Respiratory and Critical Care Medicine, 164 (5), 858-865.

Record type: Article

Abstract

Mast cells and basophils are cells that play an important role in the initiation and control of allergic inflammation in asthma and rhinitis. This study was undertaken to determine the presence and dynamics of mast cells and basophils in the nasal and bronchial mucosa of allergic rhinitis patients after segmental bronchial provocation (SBP). Eight nonasthmatic, grass pollen-allergic rhinitis patients and eight healthy controls were included. Bronchial and nasal biopsies, as well as blood samples, were taken before (T0) and 24 h (T24) after SBP. Immunohistochemical staining was performed for mast cells (tryptase and chymase; phenotypes MCT, MCTC, MCC) and basophils (BB1). In the bronchial mucosa, the number of BB1+ cells increased significantly (p < 0.05) in allergic rhinitis patients after SBP. In the nasal mucosa, the numbers of MCC and MCTC cells decreased significantly, whereas the numbers of [BB1+] cells increased significantly in allergic rhinitis patients after SBP (p < 0.05). In blood, the number of basophils decreased (p < 0.05) and the level of interleukin (IL)-5 increased (p < 0.05) in atopic patients after SBP. No significant changes could be observed in healthy controls. This study shows that SBP in nonasthmatic allergic rhinitis patients reduces numbers of mast cells in the nose as a result of enhanced degranulation. At the same time, there is evidence for an influx of basophils from the blood into the nasal and bronchial mucosae.

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More information

Published date: 2001
Keywords: bronchoprovocation, asthma, rhinitis, biopsy, inflammation

Identifiers

Local EPrints ID: 26959
URI: https://eprints.soton.ac.uk/id/eprint/26959
ISSN: 1073-449X
PURE UUID: 48c1e83b-25f4-483a-bb38-1d54d40a3a20

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Date deposited: 28 Apr 2006
Last modified: 17 Jul 2017 16:05

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Contributors

Author: Gert-Jan Braunstahl
Author: Shelley E. Overbeek
Author: Wytske J. Fokkens
Author: Alex Kleinjan
Author: Alan R. McEuen
Author: Andrew F. Walls
Author: Henk C. Hoogsteden
Author: Jan-Bas Prins

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