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Electrospray ionisation mass spectrometric analysis of lipid restructuring in the carp (Cyprinus carpio L.) during cold acclimation

Electrospray ionisation mass spectrometric analysis of lipid restructuring in the carp (Cyprinus carpio L.) during cold acclimation
Electrospray ionisation mass spectrometric analysis of lipid restructuring in the carp (Cyprinus carpio L.) during cold acclimation
Cold acclimation of carp from 30°C to 10°C causes a restructuring of liver microsomal phospholipids characterised by increased proportions of monounsaturated fatty acid in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Here, we have used electrospray ionisation mass spectrometry (ESI-MS) to determine the patterns of alteration to individual molecular species compositions of PC, PE and phosphatidylinositol (PI) in response to gradually decreasing temperature.

The results demonstrate that cold induces precise changes to a limited number of phospholipid species, and that these changes are distinct and different for each phospholipid class. The major change for PC was increased 16:1/22:6, but for PE the species that increased was 18:1/22:6. By contrast, the PI species that increased during cold acclimation were characterised by an sn-1 monounsaturated fatty acid in combination with arachidonoyl or eicosapentaenoyl fatty acid at the sn-2 position. Analysis of acyl distribution indicates that cold only caused the accumulation of monounsaturated fatty acids at the sn-1 and not at the sn-2 position of phospholipids. These results highlight the tight and restricted range of modifications that membranes make to their phospholipid composition in response to thermal stress.
cold acclimation, carp, cyprinus carpio, phospholipid, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, monounsaturated fatty acid, esi-ms
0022-0949
3989-3997
Brooks, S.
8b7562d9-af81-475a-acd6-b141e58c36d3
Clark, G.T.
7a3aed07-f59f-4a97-92ad-1658c89c8a57
Wright, S.M.
ac9e717d-e8aa-4eef-8176-75c0f2692ec3
Trueman, R.J.
876c606f-4f5f-4010-a940-149b3ab6f755
Postle, A.D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Cossins, A.R.
6f688bd6-58cd-4793-a6db-dfece9098657
Maclean, N.M.
0dd99797-2b09-42f5-b2f6-d508bfa0b14a
Brooks, S.
8b7562d9-af81-475a-acd6-b141e58c36d3
Clark, G.T.
7a3aed07-f59f-4a97-92ad-1658c89c8a57
Wright, S.M.
ac9e717d-e8aa-4eef-8176-75c0f2692ec3
Trueman, R.J.
876c606f-4f5f-4010-a940-149b3ab6f755
Postle, A.D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Cossins, A.R.
6f688bd6-58cd-4793-a6db-dfece9098657
Maclean, N.M.
0dd99797-2b09-42f5-b2f6-d508bfa0b14a

Brooks, S., Clark, G.T., Wright, S.M., Trueman, R.J., Postle, A.D., Cossins, A.R. and Maclean, N.M. (2002) Electrospray ionisation mass spectrometric analysis of lipid restructuring in the carp (Cyprinus carpio L.) during cold acclimation. Journal of Experimental Biology, 205 (24), 3989-3997.

Record type: Article

Abstract

Cold acclimation of carp from 30°C to 10°C causes a restructuring of liver microsomal phospholipids characterised by increased proportions of monounsaturated fatty acid in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Here, we have used electrospray ionisation mass spectrometry (ESI-MS) to determine the patterns of alteration to individual molecular species compositions of PC, PE and phosphatidylinositol (PI) in response to gradually decreasing temperature.

The results demonstrate that cold induces precise changes to a limited number of phospholipid species, and that these changes are distinct and different for each phospholipid class. The major change for PC was increased 16:1/22:6, but for PE the species that increased was 18:1/22:6. By contrast, the PI species that increased during cold acclimation were characterised by an sn-1 monounsaturated fatty acid in combination with arachidonoyl or eicosapentaenoyl fatty acid at the sn-2 position. Analysis of acyl distribution indicates that cold only caused the accumulation of monounsaturated fatty acids at the sn-1 and not at the sn-2 position of phospholipids. These results highlight the tight and restricted range of modifications that membranes make to their phospholipid composition in response to thermal stress.

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More information

Published date: 2002
Keywords: cold acclimation, carp, cyprinus carpio, phospholipid, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, monounsaturated fatty acid, esi-ms

Identifiers

Local EPrints ID: 26966
URI: http://eprints.soton.ac.uk/id/eprint/26966
ISSN: 0022-0949
PURE UUID: a4260182-6121-4984-9b39-175c7b86c666
ORCID for A.D. Postle: ORCID iD orcid.org/0000-0001-7361-0756

Catalogue record

Date deposited: 25 Apr 2006
Last modified: 19 Nov 2019 02:04

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