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Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus

Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus
Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus
Genome segments 1 and 2 of human group C rotavirus ‘Bristol’ strain were sequenced and their gene–protein coding properties assigned. This work completed the genome sequence of a human group C rotavirus (17,910 bp) and allowed the full gene–protein coding assignment of the 11 segments of dsRNA. Gene 1 is 3309 bp in size and contains a single ORF of 3272 nucleotides, encoding a protein of 1090 amino acids in length with a predicted molecular mass of 125 kDa. Comparison of the translated sequence with cognate published mammalian group A, B and C rotavirus sequences showed 45.2, 26.4 and 92.6% identity, respectively. The sequence contains conserved amino acid motifs including the classic RNA-dependent RNA polymerase motif GDD, indicating that segment 1 encodes the group C rotavirus polymerase protein. Gene 2 is 2736 bp in size and contains a single ORF of 2655 nucleotides encoding a protein of 884 amino acids in length with a calculated molecular mass of 102 kDa. Database searches showed highest homology with VP2, the main structural component of the ‘core’ from group A rotaviruses (46% identity). Alignment of the human group C and A rotavirus VP2 proteins revealed several characteristics common to nucleic acid binding proteins. However, these features were not shared with group B rotavirus VP2.
human group c rotavirus, vp1, vp2, gene–protein coding assignment
0168-1702
179-187
Chen, Zhilin
9293b8db-40d1-47e8-aaef-6aa797c5c660
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Lau, Jeshen
c21930de-70fd-4ab5-8838-b73417f45f5a
Caul, E. Owen
177391c9-26e2-4c94-a459-d9353c2daa61
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Chen, Zhilin
9293b8db-40d1-47e8-aaef-6aa797c5c660
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Lau, Jeshen
c21930de-70fd-4ab5-8838-b73417f45f5a
Caul, E. Owen
177391c9-26e2-4c94-a459-d9353c2daa61
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Chen, Zhilin, Lambden, Paul R., Lau, Jeshen, Caul, E. Owen and Clarke, Ian N. (2002) Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus. Virus Research, 83 (1-2), 179-187. (doi:10.1016/S0168-1702(01)00442-7).

Record type: Article

Abstract

Genome segments 1 and 2 of human group C rotavirus ‘Bristol’ strain were sequenced and their gene–protein coding properties assigned. This work completed the genome sequence of a human group C rotavirus (17,910 bp) and allowed the full gene–protein coding assignment of the 11 segments of dsRNA. Gene 1 is 3309 bp in size and contains a single ORF of 3272 nucleotides, encoding a protein of 1090 amino acids in length with a predicted molecular mass of 125 kDa. Comparison of the translated sequence with cognate published mammalian group A, B and C rotavirus sequences showed 45.2, 26.4 and 92.6% identity, respectively. The sequence contains conserved amino acid motifs including the classic RNA-dependent RNA polymerase motif GDD, indicating that segment 1 encodes the group C rotavirus polymerase protein. Gene 2 is 2736 bp in size and contains a single ORF of 2655 nucleotides encoding a protein of 884 amino acids in length with a calculated molecular mass of 102 kDa. Database searches showed highest homology with VP2, the main structural component of the ‘core’ from group A rotaviruses (46% identity). Alignment of the human group C and A rotavirus VP2 proteins revealed several characteristics common to nucleic acid binding proteins. However, these features were not shared with group B rotavirus VP2.

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More information

Published date: 2002
Keywords: human group c rotavirus, vp1, vp2, gene–protein coding assignment

Identifiers

Local EPrints ID: 26983
URI: http://eprints.soton.ac.uk/id/eprint/26983
ISSN: 0168-1702
PURE UUID: 9d45cfad-c97f-401f-ad1d-c843ec5e6089
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 25 Apr 2006
Last modified: 18 Feb 2021 16:32

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