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Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects

Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects
Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects
Background Increased production of IL-4 and IL-5 and decreased production of IFN-? by CD4+ T cells has been implicated in asthma pathogenesis. However, CD8+ T cells also produce type 1 and type 2 cytokines and the relative roles of CD4+ and CD8+ T cell cytokine production in asthma have not been previously studied.
Objective To determine the production of the type 1 and type 2 cytokines by CD4+ and CD8+ T cell subsets in asthmatic and normal subjects.
Methods Intracellular cytokine staining for IL-4, -5, -10, -13 and IFN-? was analysed in peripheral blood CD4+ and CD8+ T cells from 24 atopic asthmatic and 20 normal subjects.
Results Both subsets of T cells produced all cytokines studied and there were no significant differences between CD4+ and CD8+ T cells in their capacity to produce either type 1 or type 2 cytokines. There were significantly increased frequencies of IFN-?-positive CD4+ (13.1 ± 2.4%, vs. 7.3 ± 1.4%) and CD8+ (20.0 ± 2.9%, vs. 9.6 ± 2.1%) T cells in asthmatic subjects compared with normal subjects (P < 0.05), but not in frequencies of CD4+ or CD8+ T cells staining positively for IL-4, -5, -10 or -13.
Conclusion The frequencies of peripheral blood CD8+ T cells producing type 1 and type 2 cytokines are comparable with the frequencies of CD4+ T cells. There was an increased frequency of IFN-? producing CD4+ and CD8+ T cells in asthmatic compared with normal subjects. Further studies investigating T cells derived from the airways and investigating various stages within the disease process are required to further elucidate the importance of type 2 and type 1 T cell cytokine production in the pathogenesis of human allergic disease.
0954-7894
427-433
Cho, S.H.
03b6cdad-aeae-4256-b45b-9ec40699f820
Stanciu, L.A.
0c4d1073-f1b5-4b4b-8aa0-90694922cd3e
Begishivili, T.
d74f13bf-294d-47f3-9db5-1b10775ff27d
Bates, P.J.
55701138-5431-433d-a502-a54c5fd48829
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Johnston, S.L.
f31c4376-1118-4eba-807d-9264c8df10a1
Cho, S.H.
03b6cdad-aeae-4256-b45b-9ec40699f820
Stanciu, L.A.
0c4d1073-f1b5-4b4b-8aa0-90694922cd3e
Begishivili, T.
d74f13bf-294d-47f3-9db5-1b10775ff27d
Bates, P.J.
55701138-5431-433d-a502-a54c5fd48829
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Johnston, S.L.
f31c4376-1118-4eba-807d-9264c8df10a1

Cho, S.H., Stanciu, L.A., Begishivili, T., Bates, P.J., Holgate, S.T. and Johnston, S.L. (2002) Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects. Clinical & Experimental Allergy, 32 (3), 427-433. (doi:10.1046/j.1365-2222.2002.01281.x).

Record type: Article

Abstract

Background Increased production of IL-4 and IL-5 and decreased production of IFN-? by CD4+ T cells has been implicated in asthma pathogenesis. However, CD8+ T cells also produce type 1 and type 2 cytokines and the relative roles of CD4+ and CD8+ T cell cytokine production in asthma have not been previously studied.
Objective To determine the production of the type 1 and type 2 cytokines by CD4+ and CD8+ T cell subsets in asthmatic and normal subjects.
Methods Intracellular cytokine staining for IL-4, -5, -10, -13 and IFN-? was analysed in peripheral blood CD4+ and CD8+ T cells from 24 atopic asthmatic and 20 normal subjects.
Results Both subsets of T cells produced all cytokines studied and there were no significant differences between CD4+ and CD8+ T cells in their capacity to produce either type 1 or type 2 cytokines. There were significantly increased frequencies of IFN-?-positive CD4+ (13.1 ± 2.4%, vs. 7.3 ± 1.4%) and CD8+ (20.0 ± 2.9%, vs. 9.6 ± 2.1%) T cells in asthmatic subjects compared with normal subjects (P < 0.05), but not in frequencies of CD4+ or CD8+ T cells staining positively for IL-4, -5, -10 or -13.
Conclusion The frequencies of peripheral blood CD8+ T cells producing type 1 and type 2 cytokines are comparable with the frequencies of CD4+ T cells. There was an increased frequency of IFN-? producing CD4+ and CD8+ T cells in asthmatic compared with normal subjects. Further studies investigating T cells derived from the airways and investigating various stages within the disease process are required to further elucidate the importance of type 2 and type 1 T cell cytokine production in the pathogenesis of human allergic disease.

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Published date: 2002

Identifiers

Local EPrints ID: 26985
URI: http://eprints.soton.ac.uk/id/eprint/26985
ISSN: 0954-7894
PURE UUID: 347208d4-2675-4fc4-86e7-f045b9afa899

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Date deposited: 25 Apr 2006
Last modified: 15 Oct 2021 16:32

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Contributors

Author: S.H. Cho
Author: L.A. Stanciu
Author: T. Begishivili
Author: P.J. Bates
Author: S.T. Holgate
Author: S.L. Johnston

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