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Increased interleukin-4, interleukin-5, and interferon-gamma in airway CD4+ and CD8+ T cells in atopic asthma

Increased interleukin-4, interleukin-5, and interferon-gamma in airway CD4+ and CD8+ T cells in atopic asthma
Increased interleukin-4, interleukin-5, and interferon-gamma in airway CD4+ and CD8+ T cells in atopic asthma
Increased Th2 cytokine production in asthma is widely accepted, but excess production by asthmatic human airway CD4+ T cells has not been demonstrated, nor has a relationship with disease severity. The importance of airway CD8+ T cell type 1 and type 2 cytokine production in asthma is unknown. We investigated frequencies of IFN-{gamma}, interleukin (IL)-4 and IL-5 producing CD4+ and CD8+ blood and sputum T cells from normal subjects and subjects with asthma and compared between cell subsets, subject groups, and body compartments with and without in vitro stimulation and investigated relationships between cytokine production and asthma severity. Production of IL-4, IL-5, and IFN-{gamma} by unstimulated sputum CD4+ and CD8+ T cells was increased in subjects with asthma and related to disease severity, more for CD8+ than for CD4+ T cells. Frequencies of sputum CD8+ T cells producing type 1 and type 2 cytokines were similar to those of CD4+ T cells. In vitro stimulation polarized peripheral blood cytokine production toward IFN-{gamma} production, significantly more in subjects with asthma than in normal subjects. These data demonstrate increased type 1 and 2 cytokine production in CD4+ and CD8+ T cells in sputum and relate production to disease severity. Findings in blood did not reflect those in airways.
asthma, flow cytometry, human, sputum, t lymphocytes
1073-449X
224-230
Cho, Sang-Heon
1fe9b7ee-da4d-4a4c-ad6c-16b3269f4610
Stanciu, Luminita A.
92eb55fa-d20e-4d3a-aa71-207eaf6bceca
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Johnston, Sebastian L.
90e0ef79-cfde-40e0-b301-90d3063ee036
Cho, Sang-Heon
1fe9b7ee-da4d-4a4c-ad6c-16b3269f4610
Stanciu, Luminita A.
92eb55fa-d20e-4d3a-aa71-207eaf6bceca
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Johnston, Sebastian L.
90e0ef79-cfde-40e0-b301-90d3063ee036

Cho, Sang-Heon, Stanciu, Luminita A., Holgate, Stephen T. and Johnston, Sebastian L. (2005) Increased interleukin-4, interleukin-5, and interferon-gamma in airway CD4+ and CD8+ T cells in atopic asthma. American Journal of Respiratory and Critical Care Medicine, 171 (3), 224-230. (doi:10.1164/rccm.200310-1416OC).

Record type: Article

Abstract

Increased Th2 cytokine production in asthma is widely accepted, but excess production by asthmatic human airway CD4+ T cells has not been demonstrated, nor has a relationship with disease severity. The importance of airway CD8+ T cell type 1 and type 2 cytokine production in asthma is unknown. We investigated frequencies of IFN-{gamma}, interleukin (IL)-4 and IL-5 producing CD4+ and CD8+ blood and sputum T cells from normal subjects and subjects with asthma and compared between cell subsets, subject groups, and body compartments with and without in vitro stimulation and investigated relationships between cytokine production and asthma severity. Production of IL-4, IL-5, and IFN-{gamma} by unstimulated sputum CD4+ and CD8+ T cells was increased in subjects with asthma and related to disease severity, more for CD8+ than for CD4+ T cells. Frequencies of sputum CD8+ T cells producing type 1 and type 2 cytokines were similar to those of CD4+ T cells. In vitro stimulation polarized peripheral blood cytokine production toward IFN-{gamma} production, significantly more in subjects with asthma than in normal subjects. These data demonstrate increased type 1 and 2 cytokine production in CD4+ and CD8+ T cells in sputum and relate production to disease severity. Findings in blood did not reflect those in airways.

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More information

Published date: 2005
Keywords: asthma, flow cytometry, human, sputum, t lymphocytes

Identifiers

Local EPrints ID: 26986
URI: http://eprints.soton.ac.uk/id/eprint/26986
ISSN: 1073-449X
PURE UUID: 8c6153c5-a275-4d81-8319-3e54ec065990

Catalogue record

Date deposited: 25 Apr 2006
Last modified: 15 Jul 2019 19:13

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