The University of Southampton
University of Southampton Institutional Repository

Are cysteinyl leukotrienes involved in allergic responses in human skin

Are cysteinyl leukotrienes involved in allergic responses in human skin
Are cysteinyl leukotrienes involved in allergic responses in human skin
BackgroundCysteinyl leukotrienes have been suggested to be involved in producing the symptoms of both the early and late phases of the allergic response in the lung and other tissues.
ObjectiveTo use scanning laser Doppler imaging, microdialysis and immunocytochemistry to explore the mediator and cellular mechanisms of the dermal allergic response.
MethodsThirteen atopic volunteers received intradermal injections into the forearm of grass pollen or D. pteronyssinus extract. Changes in dermal blood flow up to 8 h were monitored by scanning laser Doppler imaging. The release of histamine, PGD2 and LTC4/D4/E4 was assessed by dermal microdialysis. Skin biopsies were taken at 6 h to determine numbers of mast cells, eosinophils, basophils, Langerhans' cells, and monocytes/macrophages, and the expression of COX-1, COX-2, 5-LO and FLAP.
ResultsAllergen provocation produced an immediate weal and flare response followed by an erythematous induration peaking at 6 h. During the first hour, c. 84 pmoles of histamine and c. 0.3 pmoles of PGD2 were recovered by microdialysis (both P < 0.001) but LTC4/D4/E4 was undetectable. No histamine, PGD2 or LTC4/D4/E4 was detectable at later times. Immunocytochemical examination of biopsies taken at 8 h showed increased numbers of eosinophils and basophils and in COX-2, 5-LO and FLAP, but not COX-1. Expression of 5-LO and FLAP was associated primarily with eosinophils.
ConclusionsThese findings suggest that inflammatory cells recruited to the site of allergen injection are not activated to release detectable amounts of cysteinyl leukotrienes. Hence, it is unlikely that the late-phase erythematous induration is mediated by this autocoid.
0954-7894
1013-1019
Church, M.K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Griffiths, T.J.
4681b3f6-5884-448d-927f-d371737f2234
Jeffery, S.
3126c6b9-f643-4f84-9730-eede3ed85dc8
Ravell, L.C.
64f34c78-9086-49cb-9df9-7cdf7df307ab
Cowburn, A.S.
f6ed371b-8671-43b2-a7cd-4e4f4a4a0abe
Sampson, A.P.
4ca76f6f-ff35-425d-a7e7-c2bd2ea2df60
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494
Church, M.K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Griffiths, T.J.
4681b3f6-5884-448d-927f-d371737f2234
Jeffery, S.
3126c6b9-f643-4f84-9730-eede3ed85dc8
Ravell, L.C.
64f34c78-9086-49cb-9df9-7cdf7df307ab
Cowburn, A.S.
f6ed371b-8671-43b2-a7cd-4e4f4a4a0abe
Sampson, A.P.
4ca76f6f-ff35-425d-a7e7-c2bd2ea2df60
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494

Church, M.K., Griffiths, T.J., Jeffery, S., Ravell, L.C., Cowburn, A.S., Sampson, A.P. and Clough, G.F. (2002) Are cysteinyl leukotrienes involved in allergic responses in human skin. Clinical & Experimental Allergy, 32 (7), 1013-1019. (doi:10.1046/j.1365-2222.2002.01436.x).

Record type: Article

Abstract

BackgroundCysteinyl leukotrienes have been suggested to be involved in producing the symptoms of both the early and late phases of the allergic response in the lung and other tissues.
ObjectiveTo use scanning laser Doppler imaging, microdialysis and immunocytochemistry to explore the mediator and cellular mechanisms of the dermal allergic response.
MethodsThirteen atopic volunteers received intradermal injections into the forearm of grass pollen or D. pteronyssinus extract. Changes in dermal blood flow up to 8 h were monitored by scanning laser Doppler imaging. The release of histamine, PGD2 and LTC4/D4/E4 was assessed by dermal microdialysis. Skin biopsies were taken at 6 h to determine numbers of mast cells, eosinophils, basophils, Langerhans' cells, and monocytes/macrophages, and the expression of COX-1, COX-2, 5-LO and FLAP.
ResultsAllergen provocation produced an immediate weal and flare response followed by an erythematous induration peaking at 6 h. During the first hour, c. 84 pmoles of histamine and c. 0.3 pmoles of PGD2 were recovered by microdialysis (both P < 0.001) but LTC4/D4/E4 was undetectable. No histamine, PGD2 or LTC4/D4/E4 was detectable at later times. Immunocytochemical examination of biopsies taken at 8 h showed increased numbers of eosinophils and basophils and in COX-2, 5-LO and FLAP, but not COX-1. Expression of 5-LO and FLAP was associated primarily with eosinophils.
ConclusionsThese findings suggest that inflammatory cells recruited to the site of allergen injection are not activated to release detectable amounts of cysteinyl leukotrienes. Hence, it is unlikely that the late-phase erythematous induration is mediated by this autocoid.

This record has no associated files available for download.

More information

Published date: 2002

Identifiers

Local EPrints ID: 26989
URI: http://eprints.soton.ac.uk/id/eprint/26989
ISSN: 0954-7894
PURE UUID: 9709c4c2-3bb2-4149-9563-5dbd02ba813f
ORCID for A.P. Sampson: ORCID iD orcid.org/0009-0008-9653-8935
ORCID for G.F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

Catalogue record

Date deposited: 25 Apr 2006
Last modified: 16 Mar 2024 02:54

Export record

Altmetrics

Contributors

Author: M.K. Church
Author: T.J. Griffiths
Author: S. Jeffery
Author: L.C. Ravell
Author: A.S. Cowburn
Author: A.P. Sampson ORCID iD
Author: G.F. Clough ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×