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Bilayer lipid membranes from falling droplets

Zagnoni, Michele, Sandison, Mairi E., Marius, Phedra and Morgan, Hywel (2009) Bilayer lipid membranes from falling droplets Analytical and Bioanalytical Chemistry, 393, pp. 1601-1605.

Record type: Article

Abstract

We describe a system that provides a rapid and simple way of forming suspended lipid bilayers within a microfluidic platform from an aqueous droplet. Bilayer lipid membranes are created in a polymeric device by contacting monolayers formed at a two-phase liquid-liquid interface. Microdroplets, containing membrane proteins, are injected onto an electrode positioned above an aperture machined through a conical cavity that is filled with a lipid-alkane solution. The formation of the BLM depends solely on the device geometry and leads to spontaneous formation of lipid bilayers simply by dispensing droplets of buffer. When an aqueous droplet containing transmembrane proteins or proteoliposomes is injected, straightforward electrophysiology measurements are possible. This method is suitable for incorporation into lab-on-a-chip devices and allows for buffer exchange and electrical measurements.

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More information

Published date: 2009
Additional Information: Imported from ISI Web of Science
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 269924
URI: http://eprints.soton.ac.uk/id/eprint/269924
ISSN: 1618-2642
PURE UUID: 53f796b3-ccca-4da8-b148-26d0426eac68
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 21 Apr 2010 07:46
Last modified: 18 Jul 2017 06:51

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Contributors

Author: Michele Zagnoni
Author: Mairi E. Sandison
Author: Phedra Marius
Author: Hywel Morgan ORCID iD

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