The University of Southampton
University of Southampton Institutional Repository

Effects of blood flow on the in vivo recovery of a small diffusible molecule by microdialysis in human skin

Clough, G.F., Boutsiouki, P., Church, M.K. and Michel, C.C. (2002) Effects of blood flow on the in vivo recovery of a small diffusible molecule by microdialysis in human skin The Journal of Pharmacology and Experimental Therapeutics, 302, (2), pp. 681-686.

Record type: Article

Abstract

The aim of this study was to investigate the impact of changes in local blood flow on the recovery of a small, diffusible molecule (sodium fluorescein) from the extravascular tissue space of the skin, by microdialysis in vivo. Loss and recovery of fluorescein by linear microdialysis probes (5-kDa molecular mass cutoff, 0.2 mm diameter) inserted 1 mm apart in pairs, at three sites in the skin of the volar surface of the forearm of healthy volunteers, was measured under conditions of basal, reduced (noradrenaline, 0.005 mg/ml), and increased (glyceryl trinitrate, patch) blood flow.
Whereas loss of tracer from the delivery probe appeared unaffected by changes in local blood flow, retrieval of fluorescein by the second probe was directly related to blood flux, measured using scanning laser Doppler imaging. Steady-state recovery at vasoconstricted sites was 4.0 ± 0.7 µg · ml1 compared with 1.8 ± 0.7 µg · ml1 at control sites (p < 0.001). Local vasodilatation reduced the retrieval of fluorescein by ~50% to give a steady-state concentration of fluorescein in the dialysate at 40 to 50 min after the start of perfusion of 0.9 ± 0.3 µg · ml1 (p = 0.05). These studies in the skin are consistent with microdialysis theory. They suggest that clearance of solute by the blood will have a significant impact on microdialysis probe recovery and that, in the skin, the magnitude of this clearance is directly related to blood flow.

Full text not available from this repository.

More information

Published date: 2002

Identifiers

Local EPrints ID: 26995
URI: http://eprints.soton.ac.uk/id/eprint/26995
ISSN: 0022-3565
PURE UUID: 8ab61e18-1824-43e1-bba7-b993efbc50f9
ORCID for G.F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

Catalogue record

Date deposited: 25 Apr 2006
Last modified: 17 Jul 2017 16:05

Export record

Contributors

Author: G.F. Clough ORCID iD
Author: P. Boutsiouki
Author: M.K. Church
Author: C.C. Michel

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×