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Inhibition by glucocorticoids of the mast cell-dependent weal and flare response in human skin in vivo

Inhibition by glucocorticoids of the mast cell-dependent weal and flare response in human skin in vivo
Inhibition by glucocorticoids of the mast cell-dependent weal and flare response in human skin in vivo
This study examines the relative contributions made by inhibition of mast cell degranulation, reduction of mast cell recruitment and maturation, and lowering the responsiveness of the vasculature to histamine, in the inhibition by glucocorticoids of the weal and flare in human skin. One forearm of healthy human volunteers was treated for 24 h (n=6) or daily for 21 days (n=10) with 0.05% clobetasol propionate. The other arm served as control. Weal and flare responses were elicited by intradermal injection of 20 l of 0.3 mM codeine. The areas of the responses were measured using scanning laser Doppler imaging. Microdialysis was used to assess histamine release. Mast cell numbers and tissue histamine content were assessed in 4-mm punch biopsies. Histamine (20 l of 1 M i.d.) was used to assess the status of the vasculature. No significant effects were seen at 24 h. At 21 days, clobetasol reduced the areas of the codeine-induced weal and flare responses by 59 and 58% respectively (both P=0.006). Mast cell numbers were reduced by 47%, (P=0.014) and total tissue histamine content by 52% (P=0.006). Codeine-induced histamine release was reduced by 44% (P=0.022). The weal, but not the flare, induced by histamine was significantly inhibited (P=0.019). Echography revealed a 15% thinning of the skin by clobetasol. These results demonstrate that reduction of the weal and flare responses to codeine following clobetasol treatment, results primarily from reduced mast cell numbers and tissue histamine content rather than inhibition by corticosteroids of mast cell degranulation.
0007-1188
286-292
Cole, Z.A.
c070462a-a9c1-48a3-ad61-089e6fefa1a6
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494
Church, M.K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Cole, Z.A.
c070462a-a9c1-48a3-ad61-089e6fefa1a6
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494
Church, M.K.
dad189d5-866e-4ae1-b005-0d87f74282b8

Cole, Z.A., Clough, G.F. and Church, M.K. (2001) Inhibition by glucocorticoids of the mast cell-dependent weal and flare response in human skin in vivo. British Journal of Pharmacology, 132 (1), 286-292. (doi:10.1038/sj.bjp.0703789).

Record type: Article

Abstract

This study examines the relative contributions made by inhibition of mast cell degranulation, reduction of mast cell recruitment and maturation, and lowering the responsiveness of the vasculature to histamine, in the inhibition by glucocorticoids of the weal and flare in human skin. One forearm of healthy human volunteers was treated for 24 h (n=6) or daily for 21 days (n=10) with 0.05% clobetasol propionate. The other arm served as control. Weal and flare responses were elicited by intradermal injection of 20 l of 0.3 mM codeine. The areas of the responses were measured using scanning laser Doppler imaging. Microdialysis was used to assess histamine release. Mast cell numbers and tissue histamine content were assessed in 4-mm punch biopsies. Histamine (20 l of 1 M i.d.) was used to assess the status of the vasculature. No significant effects were seen at 24 h. At 21 days, clobetasol reduced the areas of the codeine-induced weal and flare responses by 59 and 58% respectively (both P=0.006). Mast cell numbers were reduced by 47%, (P=0.014) and total tissue histamine content by 52% (P=0.006). Codeine-induced histamine release was reduced by 44% (P=0.022). The weal, but not the flare, induced by histamine was significantly inhibited (P=0.019). Echography revealed a 15% thinning of the skin by clobetasol. These results demonstrate that reduction of the weal and flare responses to codeine following clobetasol treatment, results primarily from reduced mast cell numbers and tissue histamine content rather than inhibition by corticosteroids of mast cell degranulation.

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Published date: 2001

Identifiers

Local EPrints ID: 26997
URI: https://eprints.soton.ac.uk/id/eprint/26997
ISSN: 0007-1188
PURE UUID: 127ab515-6789-4c6f-9af0-88ebff607910
ORCID for G.F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

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Date deposited: 25 Apr 2006
Last modified: 15 Jul 2019 19:13

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