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Basophils infiltrate human gastric mucosa at sites of Helicobacter pylori infection, and exhibit chemotaxis in response to H. pylori-derived peptide Hp(2-20)

DePaulis, Amato, Prevete, Nella, Fiorentino, Isabella, Walls, Andrew F., Curto, Monica, Petraroli, Angelica, Castaldo, Vincenza, Ceppa, Paola, Fiocca, Roberto and Marone, Gianni (2004) Basophils infiltrate human gastric mucosa at sites of Helicobacter pylori infection, and exhibit chemotaxis in response to H. pylori-derived peptide Hp(2-20) Journal of Immunology, 172, (12), pp. 7734-7743.

Record type: Article

Abstract

Basophils, which are normally confined to the circulation, can migrate to sites of allergic inflammation. Using the specific mAb, BB1, we detected basophil infiltration of the gastric mucosa of Helicobacter pylori-infected patients affected by moderate and severe gastritis. Basophils were not found in H. pylori-free individuals or in subjects with mild gastritis. The H. pylori-derived peptide, Hp(2–20), was a potent basophil chemoattractant in vitro, whereas the control peptide, Hp1, was ineffective. Basophils from peripheral blood of healthy volunteers expressed mRNA for the formyl peptide receptors, N-formyl-peptide receptor (FPR), FPR-like (FPRL)1, and FPRL2.
Preincubation of basophils with FMLP or Hp(2–20) caused complete desensitization to a subsequent challenge with homologous stimulus. Incubation of basophils with a low concentration of FMLP, which binds with high affinity to FPR, but not to FPRL1 or FPRL2, did not affect the chemotactic response to Hp(2–20). In contrast, a high concentration of FMLP, which binds to FPRL1 and FPRL2, reduced the chemotactic response to Hp(2–20). The FPR antagonist, cyclosporin H, prevented chemotaxis induced by FMLP, but not by Hp(2–20). Hp(2–20) could be responsible, at least in part, for basophil infiltration of the gastric mucosa of H. pylori-infected patients presumably through the interaction with FPRL1 and FPRL2.

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Published date: 2004

Identifiers

Local EPrints ID: 27019
URI: http://eprints.soton.ac.uk/id/eprint/27019
ISSN: 0022-1767
PURE UUID: b438912c-541b-4399-b13d-e9701a629849

Catalogue record

Date deposited: 26 Apr 2006
Last modified: 17 Jul 2017 16:05

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Contributors

Author: Amato DePaulis
Author: Nella Prevete
Author: Isabella Fiorentino
Author: Andrew F. Walls
Author: Monica Curto
Author: Angelica Petraroli
Author: Vincenza Castaldo
Author: Paola Ceppa
Author: Roberto Fiocca
Author: Gianni Marone

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