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Basophils infiltrate human gastric mucosa at sites of Helicobacter pylori infection, and exhibit chemotaxis in response to H. pylori-derived peptide Hp(2-20)

Basophils infiltrate human gastric mucosa at sites of Helicobacter pylori infection, and exhibit chemotaxis in response to H. pylori-derived peptide Hp(2-20)
Basophils infiltrate human gastric mucosa at sites of Helicobacter pylori infection, and exhibit chemotaxis in response to H. pylori-derived peptide Hp(2-20)
Basophils, which are normally confined to the circulation, can migrate to sites of allergic inflammation. Using the specific mAb, BB1, we detected basophil infiltration of the gastric mucosa of Helicobacter pylori-infected patients affected by moderate and severe gastritis. Basophils were not found in H. pylori-free individuals or in subjects with mild gastritis. The H. pylori-derived peptide, Hp(2–20), was a potent basophil chemoattractant in vitro, whereas the control peptide, Hp1, was ineffective. Basophils from peripheral blood of healthy volunteers expressed mRNA for the formyl peptide receptors, N-formyl-peptide receptor (FPR), FPR-like (FPRL)1, and FPRL2.
Preincubation of basophils with FMLP or Hp(2–20) caused complete desensitization to a subsequent challenge with homologous stimulus. Incubation of basophils with a low concentration of FMLP, which binds with high affinity to FPR, but not to FPRL1 or FPRL2, did not affect the chemotactic response to Hp(2–20). In contrast, a high concentration of FMLP, which binds to FPRL1 and FPRL2, reduced the chemotactic response to Hp(2–20). The FPR antagonist, cyclosporin H, prevented chemotaxis induced by FMLP, but not by Hp(2–20). Hp(2–20) could be responsible, at least in part, for basophil infiltration of the gastric mucosa of H. pylori-infected patients presumably through the interaction with FPRL1 and FPRL2.
0022-1767
7734-7743
DePaulis, Amato
d2dc0b83-5731-412d-a58f-012eec6ea9b7
Prevete, Nella
c2404c58-66a0-41ab-949b-a4605f287d57
Fiorentino, Isabella
ff4bf290-f84e-4aff-841c-91756ed7ac15
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Curto, Monica
aaa98a4a-5142-4e40-bd99-1b3ff5758dab
Petraroli, Angelica
3087239f-f0b9-403e-9084-c4a4063405f4
Castaldo, Vincenza
40d5264b-3d16-4c75-ab7b-4bce0a248607
Ceppa, Paola
1611e5b1-1c33-4605-a481-ef93032b7f3b
Fiocca, Roberto
af874ae9-b2f4-4dba-be01-32eed738c198
Marone, Gianni
82a7d988-43c9-423c-bcd0-7871ea7632c6
DePaulis, Amato
d2dc0b83-5731-412d-a58f-012eec6ea9b7
Prevete, Nella
c2404c58-66a0-41ab-949b-a4605f287d57
Fiorentino, Isabella
ff4bf290-f84e-4aff-841c-91756ed7ac15
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Curto, Monica
aaa98a4a-5142-4e40-bd99-1b3ff5758dab
Petraroli, Angelica
3087239f-f0b9-403e-9084-c4a4063405f4
Castaldo, Vincenza
40d5264b-3d16-4c75-ab7b-4bce0a248607
Ceppa, Paola
1611e5b1-1c33-4605-a481-ef93032b7f3b
Fiocca, Roberto
af874ae9-b2f4-4dba-be01-32eed738c198
Marone, Gianni
82a7d988-43c9-423c-bcd0-7871ea7632c6

DePaulis, Amato, Prevete, Nella, Fiorentino, Isabella, Walls, Andrew F., Curto, Monica, Petraroli, Angelica, Castaldo, Vincenza, Ceppa, Paola, Fiocca, Roberto and Marone, Gianni (2004) Basophils infiltrate human gastric mucosa at sites of Helicobacter pylori infection, and exhibit chemotaxis in response to H. pylori-derived peptide Hp(2-20). The Journal of Immunology, 172 (12), 7734-7743.

Record type: Article

Abstract

Basophils, which are normally confined to the circulation, can migrate to sites of allergic inflammation. Using the specific mAb, BB1, we detected basophil infiltration of the gastric mucosa of Helicobacter pylori-infected patients affected by moderate and severe gastritis. Basophils were not found in H. pylori-free individuals or in subjects with mild gastritis. The H. pylori-derived peptide, Hp(2–20), was a potent basophil chemoattractant in vitro, whereas the control peptide, Hp1, was ineffective. Basophils from peripheral blood of healthy volunteers expressed mRNA for the formyl peptide receptors, N-formyl-peptide receptor (FPR), FPR-like (FPRL)1, and FPRL2.
Preincubation of basophils with FMLP or Hp(2–20) caused complete desensitization to a subsequent challenge with homologous stimulus. Incubation of basophils with a low concentration of FMLP, which binds with high affinity to FPR, but not to FPRL1 or FPRL2, did not affect the chemotactic response to Hp(2–20). In contrast, a high concentration of FMLP, which binds to FPRL1 and FPRL2, reduced the chemotactic response to Hp(2–20). The FPR antagonist, cyclosporin H, prevented chemotaxis induced by FMLP, but not by Hp(2–20). Hp(2–20) could be responsible, at least in part, for basophil infiltration of the gastric mucosa of H. pylori-infected patients presumably through the interaction with FPRL1 and FPRL2.

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Published date: 2004

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Local EPrints ID: 27019
URI: https://eprints.soton.ac.uk/id/eprint/27019
ISSN: 0022-1767
PURE UUID: b438912c-541b-4399-b13d-e9701a629849

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Date deposited: 26 Apr 2006
Last modified: 15 Jul 2019 19:13

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Contributors

Author: Amato DePaulis
Author: Nella Prevete
Author: Isabella Fiorentino
Author: Andrew F. Walls
Author: Monica Curto
Author: Angelica Petraroli
Author: Vincenza Castaldo
Author: Paola Ceppa
Author: Roberto Fiocca
Author: Gianni Marone

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