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Differential roles of IL-16 and CD28/B7 costimulation in the generation of T-lymphocyte chemotactic activity in the bronchial mucosa of mild and moderate asthmatic individuals

Dent, Gordon, Hosking, Lisa A., Lordan, James L., Steel, Mark D., Cruikshank, William W., Center, David M., Ellis, Jonathan H., Holgate, Stephen T., Davies, Donna E. and Djukanovic, Ratko (2002) Differential roles of IL-16 and CD28/B7 costimulation in the generation of T-lymphocyte chemotactic activity in the bronchial mucosa of mild and moderate asthmatic individuals Journal of Allergy and Clinical Immunology, 110, (6), pp. 906-914. (doi:10.1067/mai.2002.130049).

Record type: Article


Background: IL-16 is an important T-cell chemotactic cytokine in asthmatic airways; its release from allergen-stimulated bronchial mucosa in mild asthma has been shown to be dependent on CD28/B7 costimulation.
Objective: We have extended our previous studies to investigate the role of IL-16 and CD28/B7 costimulation in T-lymphocyte chemotactic activity (TLCA) released from the bronchial mucosa in more severe asthma.
Methods: TLCA was determined in the supernatants of induced sputum and allergen-stimulated bronchial mucosal explants from healthy volunteers and volunteers with mild and moderately severe asthma by means of a Boyden chamber technique. The contribution of IL-16 to the activity was evaluated through use of a neutralizing monoclonal antibody; the contribution of CD28/B7 costimulation to allergen-induced release of TLCA was determined through use of CTLA4-Ig fusion protein and neutralizing monoclonal antibodies to CD80 (B7.1) and CD86 (B7.2).
Results: Induced sputum and unstimulated explants from asthmatic subjects generated significant spontaneous TLCA (P < .05). Both mild and moderate asthmatic explants showed significantly elevated Dermatophagoides pteronyssinus–induced release of TLCA, but only in mild asthma could sputum and allergen-stimulated explant TLCA be inhibited by anti–IL-16 (median inhibition, 39% and 59%; P < .05). In addition, allergen released significant quantities of IL-16 from mild asthmatic explants (P < .05) but not from moderate asthmatic explants. Antibodies to the CD28 counter-ligands CD80 and CD86 inhibited allergen-induced release of TLCA in mild asthmatic explants by 94% (P < .05) and 62%, but TLCA release from moderate asthmatic explants was unaffected by CTLA4-Ig.
Conclusion: These results show that TLCA release in moderate asthmatic airways, in contrast to mild asthmatic airways, is not dependent on CD28/B7 costimulation and does not involve IL-16. (J Allergy Clin Immunol 2002;110:906-14.)

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Published date: 2002
Additional Information: Basic and Clinical Immunology
Keywords: allergen, asthma, bronchial explant, B7, CD28, chemotaxis, costimulation, induced sputum, interleukin 16, T lymphocytes


Local EPrints ID: 27022
ISSN: 0091-6749
PURE UUID: b25c36eb-7d88-418c-95cd-463590025134
ORCID for Ratko Djukanovic: ORCID iD

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Date deposited: 26 Apr 2006
Last modified: 17 Jul 2017 16:05

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Author: Gordon Dent
Author: Lisa A. Hosking
Author: James L. Lordan
Author: Mark D. Steel
Author: William W. Cruikshank
Author: David M. Center
Author: Jonathan H. Ellis
Author: Donna E. Davies

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