The University of Southampton
University of Southampton Institutional Repository

Differential roles of IL-16 and CD28/B7 costimulation in the generation of T-lymphocyte chemotactic activity in the bronchial mucosa of mild and moderate asthmatic individuals

Differential roles of IL-16 and CD28/B7 costimulation in the generation of T-lymphocyte chemotactic activity in the bronchial mucosa of mild and moderate asthmatic individuals
Differential roles of IL-16 and CD28/B7 costimulation in the generation of T-lymphocyte chemotactic activity in the bronchial mucosa of mild and moderate asthmatic individuals
Background: IL-16 is an important T-cell chemotactic cytokine in asthmatic airways; its release from allergen-stimulated bronchial mucosa in mild asthma has been shown to be dependent on CD28/B7 costimulation.
Objective: We have extended our previous studies to investigate the role of IL-16 and CD28/B7 costimulation in T-lymphocyte chemotactic activity (TLCA) released from the bronchial mucosa in more severe asthma.
Methods: TLCA was determined in the supernatants of induced sputum and allergen-stimulated bronchial mucosal explants from healthy volunteers and volunteers with mild and moderately severe asthma by means of a Boyden chamber technique. The contribution of IL-16 to the activity was evaluated through use of a neutralizing monoclonal antibody; the contribution of CD28/B7 costimulation to allergen-induced release of TLCA was determined through use of CTLA4-Ig fusion protein and neutralizing monoclonal antibodies to CD80 (B7.1) and CD86 (B7.2).
Results: Induced sputum and unstimulated explants from asthmatic subjects generated significant spontaneous TLCA (P < .05). Both mild and moderate asthmatic explants showed significantly elevated Dermatophagoides pteronyssinus–induced release of TLCA, but only in mild asthma could sputum and allergen-stimulated explant TLCA be inhibited by anti–IL-16 (median inhibition, 39% and 59%; P < .05). In addition, allergen released significant quantities of IL-16 from mild asthmatic explants (P < .05) but not from moderate asthmatic explants. Antibodies to the CD28 counter-ligands CD80 and CD86 inhibited allergen-induced release of TLCA in mild asthmatic explants by 94% (P < .05) and 62%, but TLCA release from moderate asthmatic explants was unaffected by CTLA4-Ig.
Conclusion: These results show that TLCA release in moderate asthmatic airways, in contrast to mild asthmatic airways, is not dependent on CD28/B7 costimulation and does not involve IL-16. (J Allergy Clin Immunol 2002;110:906-14.)
allergen, asthma, bronchial explant, B7, CD28, chemotaxis, costimulation, induced sputum, interleukin 16, T lymphocytes
0091-6749
906-914
Dent, Gordon
73559f2a-168a-4f3d-b478-f3838e77132f
Hosking, Lisa A.
b4694f28-b11b-42ef-8fbc-6836e6919fab
Lordan, James L.
15a9bdfb-bf61-4aea-a0f4-d83868eb39d0
Steel, Mark D.
4eec21ea-3917-4ee6-9456-421475502866
Cruikshank, William W.
31ebd3eb-922e-4568-848f-d4ff9905092b
Center, David M.
1151c776-9c84-4c02-8b83-04089e02b03c
Ellis, Jonathan H.
edd4a94b-cea3-4542-a0ea-645c7d233ae6
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Dent, Gordon
73559f2a-168a-4f3d-b478-f3838e77132f
Hosking, Lisa A.
b4694f28-b11b-42ef-8fbc-6836e6919fab
Lordan, James L.
15a9bdfb-bf61-4aea-a0f4-d83868eb39d0
Steel, Mark D.
4eec21ea-3917-4ee6-9456-421475502866
Cruikshank, William W.
31ebd3eb-922e-4568-848f-d4ff9905092b
Center, David M.
1151c776-9c84-4c02-8b83-04089e02b03c
Ellis, Jonathan H.
edd4a94b-cea3-4542-a0ea-645c7d233ae6
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d

Dent, Gordon, Hosking, Lisa A., Lordan, James L., Steel, Mark D., Cruikshank, William W., Center, David M., Ellis, Jonathan H., Holgate, Stephen T., Davies, Donna E. and Djukanovic, Ratko (2002) Differential roles of IL-16 and CD28/B7 costimulation in the generation of T-lymphocyte chemotactic activity in the bronchial mucosa of mild and moderate asthmatic individuals. Journal of Allergy and Clinical Immunology, 110 (6), 906-914. (doi:10.1067/mai.2002.130049).

Record type: Article

Abstract

Background: IL-16 is an important T-cell chemotactic cytokine in asthmatic airways; its release from allergen-stimulated bronchial mucosa in mild asthma has been shown to be dependent on CD28/B7 costimulation.
Objective: We have extended our previous studies to investigate the role of IL-16 and CD28/B7 costimulation in T-lymphocyte chemotactic activity (TLCA) released from the bronchial mucosa in more severe asthma.
Methods: TLCA was determined in the supernatants of induced sputum and allergen-stimulated bronchial mucosal explants from healthy volunteers and volunteers with mild and moderately severe asthma by means of a Boyden chamber technique. The contribution of IL-16 to the activity was evaluated through use of a neutralizing monoclonal antibody; the contribution of CD28/B7 costimulation to allergen-induced release of TLCA was determined through use of CTLA4-Ig fusion protein and neutralizing monoclonal antibodies to CD80 (B7.1) and CD86 (B7.2).
Results: Induced sputum and unstimulated explants from asthmatic subjects generated significant spontaneous TLCA (P < .05). Both mild and moderate asthmatic explants showed significantly elevated Dermatophagoides pteronyssinus–induced release of TLCA, but only in mild asthma could sputum and allergen-stimulated explant TLCA be inhibited by anti–IL-16 (median inhibition, 39% and 59%; P < .05). In addition, allergen released significant quantities of IL-16 from mild asthmatic explants (P < .05) but not from moderate asthmatic explants. Antibodies to the CD28 counter-ligands CD80 and CD86 inhibited allergen-induced release of TLCA in mild asthmatic explants by 94% (P < .05) and 62%, but TLCA release from moderate asthmatic explants was unaffected by CTLA4-Ig.
Conclusion: These results show that TLCA release in moderate asthmatic airways, in contrast to mild asthmatic airways, is not dependent on CD28/B7 costimulation and does not involve IL-16. (J Allergy Clin Immunol 2002;110:906-14.)

Full text not available from this repository.

More information

Published date: 2002
Additional Information: Basic and Clinical Immunology
Keywords: allergen, asthma, bronchial explant, B7, CD28, chemotaxis, costimulation, induced sputum, interleukin 16, T lymphocytes

Identifiers

Local EPrints ID: 27022
URI: http://eprints.soton.ac.uk/id/eprint/27022
ISSN: 0091-6749
PURE UUID: b25c36eb-7d88-418c-95cd-463590025134
ORCID for Donna E. Davies: ORCID iD orcid.org/0000-0002-5117-2991
ORCID for Ratko Djukanovic: ORCID iD orcid.org/0000-0001-6039-5612

Catalogue record

Date deposited: 26 Apr 2006
Last modified: 10 Dec 2019 01:59

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×