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Biological properties and cell tropism of Chp2, a bacteriophage of the obligate intracellular bacterium Chlamydophila abortus

Biological properties and cell tropism of Chp2, a bacteriophage of the obligate intracellular bacterium Chlamydophila abortus
Biological properties and cell tropism of Chp2, a bacteriophage of the obligate intracellular bacterium Chlamydophila abortus
A number of bacteriophages belonging to the Microviridae have been described infecting chlamydiae. Phylogenetic studies divide the Chlamydiaceae into two distinct genera, Chlamydia and Chlamydophila, containing three and six different species, respectively. In this work we investigated the biological properties and host range of the recently described bacteriophage Chp2 that was originally discovered in Chlamydophila abortus.
The obligate intracellular development cycle of chlamydiae has precluded the development of quantitative approaches to assay bacteriophage infectivity. Thus, we prepared hybridomas secreting monoclonal antibodies (monoclonal antibodies 40 and 55) that were specific for Chp2. We demonstrated that Chp2 binds both C. abortus elementary bodies and reticulate bodies in an enzyme-linked immunosorbent assay. Monoclonal antibodies 40 and 55 also detected bacteriophage Chp2 antigens in chlamydia-infected eukaryotic cells. We used these monoclonal antibodies to monitor the ability of Chp2 to infect all nine species of chlamydiae. Chp2 does not infect members of the genus Chlamydia (C. trachomatis, C. suis, or C. muridarum). Chp2 can infect C. abortus, C. felis, and C. pecorum but is unable to infect other members of this genus, including C. caviae and C. pneumoniae, despite the fact that these chlamydial species support the replication of very closely related bacteriophages.
0021-9193
2748-2754
Everson, J.S.
8f5e2cbc-b8f9-4ba6-9140-d726764d6c14
Garner, S.A.
0965edba-1d1f-4080-85a0-41b53d3cb553
Fane, B.
8c5a0084-118c-4370-93c6-80748b55cc73
Liu, B.L.
1e6ef423-fbfb-4c45-aee6-29b667878629
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Everson, J.S.
8f5e2cbc-b8f9-4ba6-9140-d726764d6c14
Garner, S.A.
0965edba-1d1f-4080-85a0-41b53d3cb553
Fane, B.
8c5a0084-118c-4370-93c6-80748b55cc73
Liu, B.L.
1e6ef423-fbfb-4c45-aee6-29b667878629
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Everson, J.S., Garner, S.A., Fane, B., Liu, B.L., Lambden, P.R. and Clarke, I.N. (2002) Biological properties and cell tropism of Chp2, a bacteriophage of the obligate intracellular bacterium Chlamydophila abortus. Journal of Bacteriology, 184 (10), 2748-2754. (doi:10.1128/JB.184.10.2748-2754.2002).

Record type: Article

Abstract

A number of bacteriophages belonging to the Microviridae have been described infecting chlamydiae. Phylogenetic studies divide the Chlamydiaceae into two distinct genera, Chlamydia and Chlamydophila, containing three and six different species, respectively. In this work we investigated the biological properties and host range of the recently described bacteriophage Chp2 that was originally discovered in Chlamydophila abortus.
The obligate intracellular development cycle of chlamydiae has precluded the development of quantitative approaches to assay bacteriophage infectivity. Thus, we prepared hybridomas secreting monoclonal antibodies (monoclonal antibodies 40 and 55) that were specific for Chp2. We demonstrated that Chp2 binds both C. abortus elementary bodies and reticulate bodies in an enzyme-linked immunosorbent assay. Monoclonal antibodies 40 and 55 also detected bacteriophage Chp2 antigens in chlamydia-infected eukaryotic cells. We used these monoclonal antibodies to monitor the ability of Chp2 to infect all nine species of chlamydiae. Chp2 does not infect members of the genus Chlamydia (C. trachomatis, C. suis, or C. muridarum). Chp2 can infect C. abortus, C. felis, and C. pecorum but is unable to infect other members of this genus, including C. caviae and C. pneumoniae, despite the fact that these chlamydial species support the replication of very closely related bacteriophages.

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Published date: 2002

Identifiers

Local EPrints ID: 27041
URI: http://eprints.soton.ac.uk/id/eprint/27041
ISSN: 0021-9193
PURE UUID: e250e659-9194-4e28-86fe-e58ae2cde583
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 26 Apr 2006
Last modified: 16 Mar 2024 02:33

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Contributors

Author: J.S. Everson
Author: S.A. Garner
Author: B. Fane
Author: B.L. Liu
Author: P.R. Lambden
Author: I.N. Clarke ORCID iD

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