Microfluidic array platform for simultaneous lipid bilayer membrane formation

Zagnoni, M., Sandison, M.E. and Morgan, H. (2009) Microfluidic array platform for simultaneous lipid bilayer membrane formation Biosensors & Bioelectronics, 24, (5), pp. 1235-1240. (doi:10.1016/j.bios.2008.07.022). (PMID:18760585).


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In recent years, protein array technologies have found widespread applications in proteomics. However, new methods for high-throughput analysis of protein-protein and protein-compound interactions are still required. In this paper, an array of lipid bilayer membranes formed within a microfluidic system with integrated electrodes is presented. The system is comprised of three layers that are clamped together, thus rendering the device cleanable and reusable. The device microfluidics enable the simultaneous formation of an array of lipid bilayers using a previously developed air-exposure technique, thereby avoiding the need to manually form individual bilayers. The Ag/AgCl electrodes allow for ion channel measurements, each of the sites being independently addressable. Typically, a 50% yield in simultaneous lipid bilayer formation over 12 sites was obtained and ion channel recordings have been acquired over multiple sites. This system has great potential for the development of an automatable platform of suspended lipid bilayer arrays

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.bios.2008.07.022
ISSNs: 0956-5663 (print)
Organisations: Nanoelectronics and Nanotechnology
ePrint ID: 270657
Date :
Date Event
Date Deposited: 21 Apr 2010 07:46
Last Modified: 17 Apr 2017 18:28
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/270657

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