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On-chip electrical impedance tomography for imaging biological cells

Record type: Article

Electrical impedance tomography is an imaging technology that spatially characterizes the electrical properties of an object. We present a miniaturized electrical impedance tomography system that can image the electrical conductivity distribution within a two-dimensional cell culture. A chip containing a circular 16-electrode array was fabricated using printed circuit board developing technology and used to inject current and to measure spatial voltage across the object. The signal stimulation and voltage data acquisition were performed using an impedance analyzer, operating in four-electrode mode. An open source software, EIDORS was used for image reconstruction. Finite element modelling was used to simulate the image reconstruction process by imaging two ellipsoidal phantoms in the circular 16-electrode array. The effect of the regularization parameter in the reconstruction algorithm and the influence from noise on the fidelity of the images has been numerically analyzed. Experimentally, we show reconstructed images of a multi-nuclear single cellular organism, Physarum Polycephalum, demonstrating the first step towards impedance imaging of single cells in culture. Our system provides a non-invasive lab-on-a-chip technology for spatially mapping the electrical properties of single cells, which would be significant and useful for diagnostic and clinical applications. (C) 2009 Elsevier B.V. All rights reserved.

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Citation

Sun, Tao, Tsuda, Soichiro, Zauner, Klaus-Peter and Morgan, Hywel (2010) On-chip electrical impedance tomography for imaging biological cells Biosensors & Bioelectronics, 25, (5), pp. 1109-1115. (doi:10.1016/j.bios.2009.09.036).

More information

e-pub ahead of print date: 2 October 2009
Published date: 15 January 2010
Keywords: electrical impedance tomography, cell culture, cell imaging, lab-on-a-chip
Organisations: Nanoelectronics and Nanotechnology, Agents, Interactions & Complexity

Identifiers

Local EPrints ID: 270750
URI: http://eprints.soton.ac.uk/id/eprint/270750
ISSN: 0956-5663
PURE UUID: 476d1c26-da66-4bc6-9020-17d410d9728f
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 21 Apr 2010 07:45
Last modified: 18 Jul 2017 06:51

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Contributors

Author: Tao Sun
Author: Soichiro Tsuda
Author: Klaus-Peter Zauner
Author: Hywel Morgan ORCID iD

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