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Single Cell Impedance Cytometry for Identification and Counting of CD4 T-Cells in Human Blood Using Impedance Labels

Single Cell Impedance Cytometry for Identification and Counting of CD4 T-Cells in Human Blood Using Impedance Labels
Single Cell Impedance Cytometry for Identification and Counting of CD4 T-Cells in Human Blood Using Impedance Labels
Single cell microfluidic impedance cytometry has been used to identify cells at high speed, on the basis of their dielectric properties. However, there is no electrical analogue to a fluorescent label, meaning that it is not possible to identify subpopulations of cells. We demonstrate discrimination and enumeration of antigenically defined cell subpopulations using an alternating current (AC) impedance labeling method. Small antibody conjugated beads are mixed with cells and bind to the target population, changing the electrical properties of the target subset of cells. The principle of the technique is demonstrated by identifying and enumerating the CD4 T-lymphocyte subpopulation in human whole blood. The technique represents a simple method for detecting a subpopulation of cells within a heterogeneous mix. The impedance-based antibody identification method could form the basis of simple low-cost point of care diagnostic technologies.
1455-1461
Holmes, D
562e20f0-538e-4b0a-9486-2b0ee80654ad
Morgan, H
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Holmes, D
562e20f0-538e-4b0a-9486-2b0ee80654ad
Morgan, H
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Holmes, D and Morgan, H (2010) Single Cell Impedance Cytometry for Identification and Counting of CD4 T-Cells in Human Blood Using Impedance Labels Analytical Chemistry, 82, pp. 1455-1461.

Record type: Article

Abstract

Single cell microfluidic impedance cytometry has been used to identify cells at high speed, on the basis of their dielectric properties. However, there is no electrical analogue to a fluorescent label, meaning that it is not possible to identify subpopulations of cells. We demonstrate discrimination and enumeration of antigenically defined cell subpopulations using an alternating current (AC) impedance labeling method. Small antibody conjugated beads are mixed with cells and bind to the target population, changing the electrical properties of the target subset of cells. The principle of the technique is demonstrated by identifying and enumerating the CD4 T-lymphocyte subpopulation in human whole blood. The technique represents a simple method for detecting a subpopulation of cells within a heterogeneous mix. The impedance-based antibody identification method could form the basis of simple low-cost point of care diagnostic technologies.

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More information

Published date: 2010
Additional Information: Imported from ISI Web of Science
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 270751
URI: http://eprints.soton.ac.uk/id/eprint/270751
PURE UUID: 7200c16e-a335-416f-8d9e-a0015444586e
ORCID for H Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 21 Apr 2010 07:45
Last modified: 18 Jul 2017 06:51

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Contributors

Author: D Holmes
Author: H Morgan ORCID iD

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