Is big beautiful? The continuing story of ADAM33 and asthma
Is big beautiful? The continuing story of ADAM33 and asthma
The gene encoding A Disintegrin And Metalloprotease (ADAM) 33 was the first asthma susceptibility gene to be discovered by positional cloning.1 In 460 families enriched with asthma, linkage analysis using microsatellite markers spaced 9 cM apart revealed a region on chromosome 20p13 that carried one or more asthma genes, achieving a Maximum Lod Score (MLS) of 2.24 at 9.99 cM. The addition of further markers at 1.2 cM increased the MLS to 2.94 at 12.1 cM which further rose to 3.93 when bronchial hyperresponsiveness was included in the definition of asthma despite halving the sample size, thereby exceeding the threshold for genome wide significance. Physical mapping, direct cDNA selection, and sequencing of DNA cloned into bacterial artificial chromosomes (BACs) identified 25 candidate genes.
asthma, genetics, ADAM33
263-264
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
2005
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Holgate, S.T. and Holloway, J.W.
(2005)
Is big beautiful? The continuing story of ADAM33 and asthma.
Thorax, 60 (4), .
Abstract
The gene encoding A Disintegrin And Metalloprotease (ADAM) 33 was the first asthma susceptibility gene to be discovered by positional cloning.1 In 460 families enriched with asthma, linkage analysis using microsatellite markers spaced 9 cM apart revealed a region on chromosome 20p13 that carried one or more asthma genes, achieving a Maximum Lod Score (MLS) of 2.24 at 9.99 cM. The addition of further markers at 1.2 cM increased the MLS to 2.94 at 12.1 cM which further rose to 3.93 when bronchial hyperresponsiveness was included in the definition of asthma despite halving the sample size, thereby exceeding the threshold for genome wide significance. Physical mapping, direct cDNA selection, and sequencing of DNA cloned into bacterial artificial chromosomes (BACs) identified 25 candidate genes.
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Published date: 2005
Keywords:
asthma, genetics, ADAM33
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Local EPrints ID: 27132
URI: http://eprints.soton.ac.uk/id/eprint/27132
ISSN: 0040-6376
PURE UUID: 8ccffca4-5114-429a-b34a-bfe7436b60fb
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Date deposited: 26 Apr 2006
Last modified: 09 Jan 2022 02:54
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