A comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis
A comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis
Deuterated choline-d9 labelling of IMR-32 cells enabled comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis after 96 h polyunsaturated fatty acid supplementation. Surprisingly, while cell phosphatidylcholine synthesis and remodelling reflected a pattern of polyunsaturated fatty acid accretion, the saturated endonuclear phosphatidylcholine pool was only transiently labelled with polyunsaturates. Periodic endonuclear accumulations of the lipid second messenger diacylglycerol, mobilised from unsaturated phosphatidylinositol or saturated phosphatidylcholine, accompany cell proliferation. Non-specific incorporation into endonuclear phosphatidylcholine and selective removal or remodelling of unsaturated molecular species may form part of a single ‘off switch’ recycling all endonuclear diacylglycerol accumulations.
phosphatidylcholine synthesis, IMR-32 nuclei, deuterium labelling, electrospray ionisation mass spectrometry
89-93
Hunt, Alan N.
95a3e223-da96-40e7-b47d-27dce014e305
Clark, Graeme T.
1ccb751b-3780-485a-b1de-07bbee387859
Neale, James R.
21737dff-f26a-4085-942e-e32ef77fd701
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
2002
Hunt, Alan N.
95a3e223-da96-40e7-b47d-27dce014e305
Clark, Graeme T.
1ccb751b-3780-485a-b1de-07bbee387859
Neale, James R.
21737dff-f26a-4085-942e-e32ef77fd701
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Hunt, Alan N., Clark, Graeme T., Neale, James R. and Postle, Anthony D.
(2002)
A comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis.
FEBS Letters, 530 (1-3), .
(doi:10.1016/S0014-5793(02)03429-4).
Abstract
Deuterated choline-d9 labelling of IMR-32 cells enabled comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis after 96 h polyunsaturated fatty acid supplementation. Surprisingly, while cell phosphatidylcholine synthesis and remodelling reflected a pattern of polyunsaturated fatty acid accretion, the saturated endonuclear phosphatidylcholine pool was only transiently labelled with polyunsaturates. Periodic endonuclear accumulations of the lipid second messenger diacylglycerol, mobilised from unsaturated phosphatidylinositol or saturated phosphatidylcholine, accompany cell proliferation. Non-specific incorporation into endonuclear phosphatidylcholine and selective removal or remodelling of unsaturated molecular species may form part of a single ‘off switch’ recycling all endonuclear diacylglycerol accumulations.
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Published date: 2002
Keywords:
phosphatidylcholine synthesis, IMR-32 nuclei, deuterium labelling, electrospray ionisation mass spectrometry
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Local EPrints ID: 27165
URI: http://eprints.soton.ac.uk/id/eprint/27165
ISSN: 0014-5793
PURE UUID: c6043317-e282-4ef3-b59e-dba469bb7198
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Date deposited: 26 Apr 2006
Last modified: 16 Mar 2024 02:48
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Author:
Alan N. Hunt
Author:
Graeme T. Clark
Author:
James R. Neale
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