Dynamic lipidomic insights into phosphatidylcholine synthesis from organelle to organism
Dynamic lipidomic insights into phosphatidylcholine synthesis from organelle to organism
Recent technical improvement and technological innovation in small molecule mass spectrometry have provided powerful tools for the intensive metabolomic biochemical investigations that will necessarily characterise the "post-genomic" era of biomedical research. For membrane phospholipids, use of tandem electrospray ionisation mass spectrometry (ESI-MS/MS) exploiting precursor scanning of class-specific diagnostic fragments, can provide detailed quantitative profiles at the level of individual molecular species for many hundreds of unique lipids. Such "snapshot" measurements provide little information concerning metabolic flux. However, recent use of metabolic labelling with stable isotope derivatives of phospholipid headgroups combined with precursor scans of unlabelled and labelled fragments have yielded considerable insight into phosphatidylcholine metabolism in vivo. Here, we briefly review some of the recent work on pathways of phosphatidylcholine metabolism ranging from studies at subcellular organelle level through to whole organism. The sensitivity, specificity and suitability of this powerful methodological approach to numerous questions of phospholipid metabolism place ESI-MS/MS at the very heart of dynamic lipidomics in the foreseeable future.
Electrospray ionisation mass spectrometry, phospholipid, phosphatidylcholine, molecular species, lipidomics, stable isotope
127-135
Hunt, Alan N.
95a3e223-da96-40e7-b47d-27dce014e305
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
2005
Hunt, Alan N.
95a3e223-da96-40e7-b47d-27dce014e305
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Hunt, Alan N. and Postle, Anthony D.
(2005)
Dynamic lipidomic insights into phosphatidylcholine synthesis from organelle to organism.
Spectroscopy, 19, .
Abstract
Recent technical improvement and technological innovation in small molecule mass spectrometry have provided powerful tools for the intensive metabolomic biochemical investigations that will necessarily characterise the "post-genomic" era of biomedical research. For membrane phospholipids, use of tandem electrospray ionisation mass spectrometry (ESI-MS/MS) exploiting precursor scanning of class-specific diagnostic fragments, can provide detailed quantitative profiles at the level of individual molecular species for many hundreds of unique lipids. Such "snapshot" measurements provide little information concerning metabolic flux. However, recent use of metabolic labelling with stable isotope derivatives of phospholipid headgroups combined with precursor scans of unlabelled and labelled fragments have yielded considerable insight into phosphatidylcholine metabolism in vivo. Here, we briefly review some of the recent work on pathways of phosphatidylcholine metabolism ranging from studies at subcellular organelle level through to whole organism. The sensitivity, specificity and suitability of this powerful methodological approach to numerous questions of phospholipid metabolism place ESI-MS/MS at the very heart of dynamic lipidomics in the foreseeable future.
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Published date: 2005
Keywords:
Electrospray ionisation mass spectrometry, phospholipid, phosphatidylcholine, molecular species, lipidomics, stable isotope
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Local EPrints ID: 27170
URI: http://eprints.soton.ac.uk/id/eprint/27170
ISSN: 0712-4813
PURE UUID: 8b0e9ca3-7a29-42ed-806e-31c296a8a81f
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Date deposited: 26 Apr 2006
Last modified: 08 Jan 2022 02:41
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Author:
Alan N. Hunt
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