Polymorphism of the mast cell chymase gene (CMA1) promoter region: lack of association with asthma but association with serum total immunoglobulin E levels in adult atopic dermatitis
Polymorphism of the mast cell chymase gene (CMA1) promoter region: lack of association with asthma but association with serum total immunoglobulin E levels in adult atopic dermatitis
Background Mast cell chymase has the potential to be an important mediator of inflammation and remodelling in the asthmatic lung. Previous studies have examined association between promoter polymorphism of the chymase gene (CMA1) and allergic phenotypes but the significance of this polymorphism is unclear. We have examined association of a CMA1 variant in relation to asthma in a large UK Caucasian family cohort.
Methods A polymorphism of the CMA1 gene promoter (?1903G/A) was genotyped in 341 asthmatic families and in 184 non-asthmatic adults recruited from the UK PCR-RFLP based genotyping. Association with asthma diagnosis, atopy, specific and total IgE, and atopy and asthma severity was examined.
Results Case–control studies did not reveal a significant difference in allele frequency between asthmatics and controls. A significant association was found between CMA1 genotypes and total IgE levels in subjects with self-reported eczema that remained significant after correction for multiple testing (median total serum IgE GG 297 kU/L, GA 144 kU/L, AA 48.4 kU/L, Pc=0.0032).
Conclusion These data suggest that CMA1 promoter polymorphism does not contribute to asthma susceptibility or severity but may be involved in regulating IgE levels in patients with eczema.
1037-1042
Iwanaga, T.
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McEuen, A.
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Walls, A.F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Clough, J.B.
54c3712a-f495-4ba6-b190-73fa0ba2b30f
Keith, T.P.
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Rorke, S.
ca4b3d19-8862-40e7-8f33-b20da825b08d
Barton, S.J.
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Holgate, S.T.
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Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
2004
Iwanaga, T.
b7f257a2-5524-4b0a-9e44-d6af314043fe
McEuen, A.
59d3ad42-84ac-47c5-abb6-d468fee7dda8
Walls, A.F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Clough, J.B.
54c3712a-f495-4ba6-b190-73fa0ba2b30f
Keith, T.P.
70e2a4c6-8750-44ea-805e-d74e3ba1739d
Rorke, S.
ca4b3d19-8862-40e7-8f33-b20da825b08d
Barton, S.J.
4f674382-ca0b-44ad-9670-e71a0b134ef0
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Iwanaga, T., McEuen, A., Walls, A.F., Clough, J.B., Keith, T.P., Rorke, S., Barton, S.J., Holgate, S.T. and Holloway, J.W.
(2004)
Polymorphism of the mast cell chymase gene (CMA1) promoter region: lack of association with asthma but association with serum total immunoglobulin E levels in adult atopic dermatitis.
Clinical & Experimental Allergy, 34 (7), .
(doi:10.1111/j.1365-2222.2004.02000.x).
Abstract
Background Mast cell chymase has the potential to be an important mediator of inflammation and remodelling in the asthmatic lung. Previous studies have examined association between promoter polymorphism of the chymase gene (CMA1) and allergic phenotypes but the significance of this polymorphism is unclear. We have examined association of a CMA1 variant in relation to asthma in a large UK Caucasian family cohort.
Methods A polymorphism of the CMA1 gene promoter (?1903G/A) was genotyped in 341 asthmatic families and in 184 non-asthmatic adults recruited from the UK PCR-RFLP based genotyping. Association with asthma diagnosis, atopy, specific and total IgE, and atopy and asthma severity was examined.
Results Case–control studies did not reveal a significant difference in allele frequency between asthmatics and controls. A significant association was found between CMA1 genotypes and total IgE levels in subjects with self-reported eczema that remained significant after correction for multiple testing (median total serum IgE GG 297 kU/L, GA 144 kU/L, AA 48.4 kU/L, Pc=0.0032).
Conclusion These data suggest that CMA1 promoter polymorphism does not contribute to asthma susceptibility or severity but may be involved in regulating IgE levels in patients with eczema.
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Published date: 2004
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Local EPrints ID: 27180
URI: http://eprints.soton.ac.uk/id/eprint/27180
ISSN: 0954-7894
PURE UUID: 4c7a637c-3f8e-4684-b1f9-07cbca9303d6
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Date deposited: 25 Apr 2006
Last modified: 16 Mar 2024 03:22
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Author:
T. Iwanaga
Author:
A. McEuen
Author:
J.B. Clough
Author:
T.P. Keith
Author:
S. Rorke
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