The University of Southampton
University of Southampton Institutional Repository

Phenotype of fetal monocytes and B lymphocytes during the third trimester of pregnancy

Record type: Article

The neonate typically exhibits an immature immune response compared with the adult, yet the fetus is able to generate antigen-specific responses from around 20–22 weeks of gestation. Although antigen-presenting cells (APCs) must have attained the necessary level of maturity to support this, very little is known about the phenotype and function of these populations during human fetal development. Whole blood flow cytometry was, therefore, utilised to phenotype fetal/neonatal circulating monocytes and B cells throughout the third trimester of pregnancy.
The percentage of B cells (CD19+) expressing MHC Class II was comparable to the adult at all gestations, whereas the percentage of MHC Class II-positive monocytes (CD14+) increased significantly over gestation (P=0.0008) but remained lower than the adult at term. In contrast, the percentage of CD40+ or CD86+ fetal/neonatal monocytes at all gestations was comparable to the adult, but there was a maturational increase in the percentage of CD40+ or CD86+ B cells (P=0.007) to adult levels by term. The expression of CD14 itself (mean fluorescence intensity, MFI) showed a trend to increase over gestation (P=0.062) and, although all CD14+ cells expressed other receptors associated with innate immune responses (CD11b and CD35), there was fluctuation in the intensity of expression over gestation. Functional immaturity of neonatal antigen-specific immune responses could be associated with reduced co-stimulation provided by both monocytes (via reduced MHC Class II) and B cells (via reduced CD40 and CD86); altered innate responsiveness of monocytes could also contribute.

Full text not available from this repository.

Citation

Jones, C.A., Holloway, J.A. and Warner, J.O. (2002) Phenotype of fetal monocytes and B lymphocytes during the third trimester of pregnancy Journal of Reproductive Immunology, 56, (1-2), pp. 45-60. (doi:10.1016/S0165-0378(02)00022-0).

More information

Published date: 2002
Keywords: monocytes, b cells, gestation, neonatal

Identifiers

Local EPrints ID: 27188
URI: http://eprints.soton.ac.uk/id/eprint/27188
ISSN: 0165-0378
PURE UUID: 79e6b1f3-6594-4b01-abcb-5ec840067bbb
ORCID for J.A. Holloway: ORCID iD orcid.org/0000-0002-2268-3071

Catalogue record

Date deposited: 27 Apr 2006
Last modified: 17 Jul 2017 16:05

Export record

Altmetrics


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×