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Expression of CD34 and L-selectin on human corneal keratocytes

Expression of CD34 and L-selectin on human corneal keratocytes
Expression of CD34 and L-selectin on human corneal keratocytes
PURPOSE. To investigate the expression of CD34, a hematopoietic stem cell marker and an adhesion molecule, and its ligand L-selectin in the human cornea.
METHODS. Seventeen normal adult human corneal specimens were studied by immunohistochemistry using a panel of monoclonal antibodies against all three classes of the hematopoietic stem cell marker CD34 and its ligand L-selectin. An additional six corneal specimens were used for protein extraction and analysis by Western blotting, using the CD34 and L-selectin antibodies. PCR was used to determine expression of mRNA for CD34 and L-selectin in the corneal specimens.
RESULTS. Only corneal keratocytes showed positive immunostaining for all three classes of CD34. Western blotting confirmed the expression of CD34 by these cells and mRNA expression for CD34 in the corneal stroma was demonstrated by PCR. For L-selectin, positive staining around keratocytes was noted on immunohistochemistry but L-selectin could not be detected either by Western blotting or PCR.
CONCLUSIONS. Normal human corneal keratocytes express all three classes of CD34. The expression of this adhesion molecule on corneal keratocytes suggests that it may have a role in keeping the keratocytes anchored in their microniche, between the collagen lamellae. The positive staining for L-selectin found by immunohistochemistry but not by Western blotting or PCR would indicate the presence of either another ligand from the selectin family or a cross-reactive epitope on corneal keratocytes.
0146-0404
4689-4692
Joseph, Annie
510fee34-fc32-4811-871b-770631166ba4
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Jham, Seema
3553b092-6541-48ae-a18a-dbd91747a035
Jones, Rhodri E.
ba16e5d7-9c33-40db-9d83-075e0e54df7b
Tighe, Paddy
a97aafd0-1fa3-4426-8c4b-c940b379d71a
McIntosh, Richard S.
bb3935a0-b4cd-4880-b55e-76598b3816e6
Dua, Harminder S.
8de6ef3b-2de2-473a-9100-515d3c2b94f6
Joseph, Annie
510fee34-fc32-4811-871b-770631166ba4
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Jham, Seema
3553b092-6541-48ae-a18a-dbd91747a035
Jones, Rhodri E.
ba16e5d7-9c33-40db-9d83-075e0e54df7b
Tighe, Paddy
a97aafd0-1fa3-4426-8c4b-c940b379d71a
McIntosh, Richard S.
bb3935a0-b4cd-4880-b55e-76598b3816e6
Dua, Harminder S.
8de6ef3b-2de2-473a-9100-515d3c2b94f6

Joseph, Annie, Hossain, Parwez, Jham, Seema, Jones, Rhodri E., Tighe, Paddy, McIntosh, Richard S. and Dua, Harminder S. (2003) Expression of CD34 and L-selectin on human corneal keratocytes. Investigative Ophthalmology & Visual Science, 44 (11), 4689-4692. (doi:10.1167/iovs.02-0999).

Record type: Article

Abstract

PURPOSE. To investigate the expression of CD34, a hematopoietic stem cell marker and an adhesion molecule, and its ligand L-selectin in the human cornea.
METHODS. Seventeen normal adult human corneal specimens were studied by immunohistochemistry using a panel of monoclonal antibodies against all three classes of the hematopoietic stem cell marker CD34 and its ligand L-selectin. An additional six corneal specimens were used for protein extraction and analysis by Western blotting, using the CD34 and L-selectin antibodies. PCR was used to determine expression of mRNA for CD34 and L-selectin in the corneal specimens.
RESULTS. Only corneal keratocytes showed positive immunostaining for all three classes of CD34. Western blotting confirmed the expression of CD34 by these cells and mRNA expression for CD34 in the corneal stroma was demonstrated by PCR. For L-selectin, positive staining around keratocytes was noted on immunohistochemistry but L-selectin could not be detected either by Western blotting or PCR.
CONCLUSIONS. Normal human corneal keratocytes express all three classes of CD34. The expression of this adhesion molecule on corneal keratocytes suggests that it may have a role in keeping the keratocytes anchored in their microniche, between the collagen lamellae. The positive staining for L-selectin found by immunohistochemistry but not by Western blotting or PCR would indicate the presence of either another ligand from the selectin family or a cross-reactive epitope on corneal keratocytes.

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More information

Published date: 2003
Organisations: Infection Inflammation & Immunity

Identifiers

Local EPrints ID: 27194
URI: http://eprints.soton.ac.uk/id/eprint/27194
ISSN: 0146-0404
PURE UUID: ca0a0209-d622-47b5-a1eb-7eaac9ce1ca9
ORCID for Parwez Hossain: ORCID iD orcid.org/0000-0002-3131-2395

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Date deposited: 27 Apr 2006
Last modified: 16 Mar 2024 03:47

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Contributors

Author: Annie Joseph
Author: Parwez Hossain ORCID iD
Author: Seema Jham
Author: Rhodri E. Jones
Author: Paddy Tighe
Author: Richard S. McIntosh
Author: Harminder S. Dua

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