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Quantitative and functional differences in CD8+ lymphocyte responses in resolved acute and chronic hepatitis C virus infection

Lancaster, T., Sanders, E., Christie, J.M.L., Brooks, C., Green, S. and Rosenberg, W.M.C. (2002) Quantitative and functional differences in CD8+ lymphocyte responses in resolved acute and chronic hepatitis C virus infection Journal of Viral Hepatitis, 9, (1), pp. 18-28. (doi:10.1046/j.1365-2893.2002.00330.x).

Record type: Article


CD8+ T lymphocyte responses are important in the clearance of viral infections. In chronic infections they may contribute to pathogenesis. To investigate the role of CD8+ T lymphocyte responses in viral clearance and chronic hepatitis C we have compared hepatitis C virus (HCV) specific cytotoxicity and interferon-gamma (IFN-?) production in patients with resolved-acute, and chronic HCV infection. CD8+ T cell responses to a panel of 13 HCV T cell peptide epitopes were studied using Elispot assays of IFN-? production and chromium release cytotoxicity assays. Responses of seven patients with resolved acute HCV infection were compared with those of 14 chronically infected patients. HCV-specific cytotoxicity differentiated the two populations of patients.
The majority (71%) of patients with resolved acute infection tested positive to 42% of relevant peptides compared with the minority (28%) of patients with chronic hepatitis C (P=0.03) who responded to only 8% of relevant peptides (P=0.0009). In contrast, HCV-specific IFN-? production was detected in 86% of patients with either resolved or chronic infection in response to 42% and 35%, respectively, of relevant peptides tested (not significant). In patients with chronic infection the magnitude of the HCV-specific IFN-? production was inversely correlated to viral load (R2=0.52; P=0.042). Failure to clear HCV infection may be attributable to the presence of noncytolytic IFN-? producing CD8+ T lymphocytes in chronically infected patients. However these CD8+ T cells may play a beneficial role in contributing to the control of viral load in chronic hepatitis C.

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Published date: 2002
Keywords: cytotoxic t, cellshcv, interferon-?


Local EPrints ID: 27218
ISSN: 1352-0504
PURE UUID: b33cd339-89a0-4d30-9d2d-f90d5252dcd8

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Date deposited: 27 Apr 2006
Last modified: 17 Jul 2017 16:05

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Author: T. Lancaster
Author: E. Sanders
Author: J.M.L. Christie
Author: C. Brooks
Author: S. Green
Author: W.M.C. Rosenberg

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