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ADAM33 polymorphism: association with bronchial hyper-responsiveness in Korean asthmatics

ADAM33 polymorphism: association with bronchial hyper-responsiveness in Korean asthmatics
ADAM33 polymorphism: association with bronchial hyper-responsiveness in Korean asthmatics
Background A disintegrin and metalloprotease 33 (ADAM33) is expressed in the lung by fibroblasts and bronchial smooth muscle cells. Given its structure and cellular provenance, ADAM33 may be associated with airway remodelling and bronchial hyper-responsiveness. Single nucleotide polymorphisms (SNPs) and haplotypes of the ADAM33 gene have previously been associated with asthma susceptibility in the Caucasian population.
Objective and Methods To assess whether genetic variants of ADAM33 are related to asthma in a Korean population, we conducted an association study of the ADAM33 gene with asthma susceptibility, bronchial hyper-reactivity and serum IgE in Korean asthmatics (n=326) and normal controls (n=151). Five of the 14 polymorphisms originally reported to be associated with asthma development (S1 G>A, T1 T>C, V-1 C>A, V1 T>A, V4 C>G) were genotyped using single base extension and electrophoresis. Haplotypes and their frequencies were inferred using the algorithm implemented by the software Arlequin. Allele frequencies of each SNP and haplotypes were compared between the patients and the normal controls using logistic regression analysis.
Results There was no significant difference in the distribution of SNPs and the six haplotypes between asthmatics and normal controls. All single SNPs and six haplotypes in ADAM33 were also analysed for the association with level of PC20 using general linear models. The distribution of the T1 T>C SNP and one haplotype (ht4: GCGG) showed significant association with log-transformed PC20 methacholine level in the asthma patients (P=0.03 and 0.0007, respectively, using a co-dominant model).
Conclusion Polymorphism of ADAM33 may contribute to development of BHR in asthma.
0954-7894
860-865
Lee, J.H.
3b88784a-02e7-4b63-b112-3fdb4e1abf4e
Park, H.S.
37395c90-bc75-4c48-9d46-daac0107844b
Park, S.W.
42cc54a3-da55-4974-b764-996b2b3bf03f
Jang, A.S.
06bca3fb-472e-4f2c-abc5-06151d666b93
Uh, S.T.
5380fb94-0070-4860-bb7d-469d41fdad3c
Rhim, T.
fd07f92d-c083-4ae4-8c99-a199e1631075
Park, C.S.
50cefa27-eae1-4536-8d92-4d3362225d84
Hong, S.J.
1b953e05-a21b-444f-8b3a-0b3168b987ac
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Shin, H.D.
06c51fc6-48d5-4795-82c4-573de62641b7
Lee, J.H.
3b88784a-02e7-4b63-b112-3fdb4e1abf4e
Park, H.S.
37395c90-bc75-4c48-9d46-daac0107844b
Park, S.W.
42cc54a3-da55-4974-b764-996b2b3bf03f
Jang, A.S.
06bca3fb-472e-4f2c-abc5-06151d666b93
Uh, S.T.
5380fb94-0070-4860-bb7d-469d41fdad3c
Rhim, T.
fd07f92d-c083-4ae4-8c99-a199e1631075
Park, C.S.
50cefa27-eae1-4536-8d92-4d3362225d84
Hong, S.J.
1b953e05-a21b-444f-8b3a-0b3168b987ac
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Shin, H.D.
06c51fc6-48d5-4795-82c4-573de62641b7

Lee, J.H., Park, H.S., Park, S.W., Jang, A.S., Uh, S.T., Rhim, T., Park, C.S., Hong, S.J., Holgate, S.T., Holloway, J.W. and Shin, H.D. (2004) ADAM33 polymorphism: association with bronchial hyper-responsiveness in Korean asthmatics. Clinical & Experimental Allergy, 34 (6), 860-865. (doi:10.1111/j.1365-2222.2004.01977.x).

Record type: Article

Abstract

Background A disintegrin and metalloprotease 33 (ADAM33) is expressed in the lung by fibroblasts and bronchial smooth muscle cells. Given its structure and cellular provenance, ADAM33 may be associated with airway remodelling and bronchial hyper-responsiveness. Single nucleotide polymorphisms (SNPs) and haplotypes of the ADAM33 gene have previously been associated with asthma susceptibility in the Caucasian population.
Objective and Methods To assess whether genetic variants of ADAM33 are related to asthma in a Korean population, we conducted an association study of the ADAM33 gene with asthma susceptibility, bronchial hyper-reactivity and serum IgE in Korean asthmatics (n=326) and normal controls (n=151). Five of the 14 polymorphisms originally reported to be associated with asthma development (S1 G>A, T1 T>C, V-1 C>A, V1 T>A, V4 C>G) were genotyped using single base extension and electrophoresis. Haplotypes and their frequencies were inferred using the algorithm implemented by the software Arlequin. Allele frequencies of each SNP and haplotypes were compared between the patients and the normal controls using logistic regression analysis.
Results There was no significant difference in the distribution of SNPs and the six haplotypes between asthmatics and normal controls. All single SNPs and six haplotypes in ADAM33 were also analysed for the association with level of PC20 using general linear models. The distribution of the T1 T>C SNP and one haplotype (ht4: GCGG) showed significant association with log-transformed PC20 methacholine level in the asthma patients (P=0.03 and 0.0007, respectively, using a co-dominant model).
Conclusion Polymorphism of ADAM33 may contribute to development of BHR in asthma.

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Published date: 2004

Identifiers

Local EPrints ID: 27221
URI: http://eprints.soton.ac.uk/id/eprint/27221
ISSN: 0954-7894
PURE UUID: 21a714ce-1cb7-47ad-8801-890c7c2f5ba4
ORCID for J.W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

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Date deposited: 25 Apr 2006
Last modified: 16 Mar 2024 02:57

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Contributors

Author: J.H. Lee
Author: H.S. Park
Author: S.W. Park
Author: A.S. Jang
Author: S.T. Uh
Author: T. Rhim
Author: C.S. Park
Author: S.J. Hong
Author: S.T. Holgate
Author: J.W. Holloway ORCID iD
Author: H.D. Shin

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