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Helium ion microscopy of Lepidoptera scales

Helium ion microscopy of Lepidoptera scales
Helium ion microscopy of Lepidoptera scales
In this report, helium ion microscopy (HIM) is used to study the micro and nano-structures responsible for structural color in the wings of two species of Lepidotera from the Papilionidae family: Papilio ulysses (Blue Mountain Butterfly) and Parides sesostris (Emerald-patched Cattleheart). Electronic charging under the beam of uncoated scales from the wings of these butterflies is successfully neutralized, leading to images displaying a large depth-of-field and a high level of surface detail, which would normally be obscured by traditional coating methods used for scanning electron microscopy (SEM). The images are compared to those from variable pressure SEM, demonstrating the superiority of HIM at high magnifications. In addition, the large depth-of-field capabilities of HIM are exploited through the creation of stereo pairs which allows the exploration of the third dimension. Furthermore, the extraction of quantitative height information which matches well with cross-sectional transmission electron microscopy (TEM) measurements from the literature is demonstrated.
helium ion microscopy, scanning electron microscopy, structural colour, charge neutralization, stereo pairs
0161-0457
107-120
Boden, Stuart
83976b65-e90f-42d1-9a01-fe9cfc571bf8
Asadollahbaik, Asa
af3047e2-2925-44c5-8d8b-7cb12a1146ee
Rutt, Harvey
e09fa327-0c01-467a-9898-4e7f0cd715fc
Bagnall, Darren
5d84abc8-77e5-43f7-97cb-e28533f25ef1
Boden, Stuart
83976b65-e90f-42d1-9a01-fe9cfc571bf8
Asadollahbaik, Asa
af3047e2-2925-44c5-8d8b-7cb12a1146ee
Rutt, Harvey
e09fa327-0c01-467a-9898-4e7f0cd715fc
Bagnall, Darren
5d84abc8-77e5-43f7-97cb-e28533f25ef1

Boden, Stuart, Asadollahbaik, Asa, Rutt, Harvey and Bagnall, Darren (2011) Helium ion microscopy of Lepidoptera scales. [in special issue: Helium Ion Microscopy] Scanning, 34 (2), 107-120. (PMID:21796646)

Record type: Article

Abstract

In this report, helium ion microscopy (HIM) is used to study the micro and nano-structures responsible for structural color in the wings of two species of Lepidotera from the Papilionidae family: Papilio ulysses (Blue Mountain Butterfly) and Parides sesostris (Emerald-patched Cattleheart). Electronic charging under the beam of uncoated scales from the wings of these butterflies is successfully neutralized, leading to images displaying a large depth-of-field and a high level of surface detail, which would normally be obscured by traditional coating methods used for scanning electron microscopy (SEM). The images are compared to those from variable pressure SEM, demonstrating the superiority of HIM at high magnifications. In addition, the large depth-of-field capabilities of HIM are exploited through the creation of stereo pairs which allows the exploration of the third dimension. Furthermore, the extraction of quantitative height information which matches well with cross-sectional transmission electron microscopy (TEM) measurements from the literature is demonstrated.

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More information

Submitted date: 18 May 2011
Published date: 27 July 2011
Keywords: helium ion microscopy, scanning electron microscopy, structural colour, charge neutralization, stereo pairs
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 272301
URI: https://eprints.soton.ac.uk/id/eprint/272301
ISSN: 0161-0457
PURE UUID: daab4c30-5e67-4c6a-8ea6-a8e605fc9a7b
ORCID for Stuart Boden: ORCID iD orcid.org/0000-0002-4232-1828

Catalogue record

Date deposited: 18 May 2011 11:07
Last modified: 06 Jun 2018 12:43

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