Cutaneous microdialysis as a novel means of continuously stimulating eccrine sweat glands in vivo
Cutaneous microdialysis as a novel means of continuously stimulating eccrine sweat glands in vivo
Previous studies of the pharmacological regulation of sweat gland function in humans have administered agonists or antagonists systemically, by local intradermal injection or by iontophoresis. This has not allowed prolonged or steady-state activation of sweat glands to be examined. In this study, we used the technique of dermal microdialysis to administer pharmacological agents singly and in combination for up to 5 hours. Muscarinic stimulation with pilocarpine nitrate (50 g ml-1 to 1.66 mg ml-1) produced a sigmoid dose response curve, with maximal sweating (measured as transepidermal water loss) (mean 70 g m-2 hour-1) after 15 minutes.
This was sustained at steady-state levels (55 g m-2 hour-1) until perfusion stopped. Perfusion with atropine (0.003 mg ml-1) reduced sweating below baseline and blocked pilocarpine-induced sweating completely. Noradrenaline (0.005 mg ml-1) induced much lower sweat rates than pilocarpine (56.81.62 g m-2 hour-1 vs 8.21.2 g m-2 hour-1, respectively, P<0.001) and this was unaffected by co-administration of atropine. This method has made it possible to show that sweat glands are capable of sustaining near maximal activity for at least 5 hours. The method has future application in investigation of conditions with disordered sweat gland activity.
tewl, transepidermal water loss
1220-1225
Morgan, Caroline J.
1901f7fd-b5c5-48ab-ba1a-71328c83ac72
Friedmann, Peter S.
d50bac23-f3ec-4493-8fa0-fa126cbeba88
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494
2006
Morgan, Caroline J.
1901f7fd-b5c5-48ab-ba1a-71328c83ac72
Friedmann, Peter S.
d50bac23-f3ec-4493-8fa0-fa126cbeba88
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494
Morgan, Caroline J., Friedmann, Peter S., Church, Martin K. and Clough, Geraldine F.
(2006)
Cutaneous microdialysis as a novel means of continuously stimulating eccrine sweat glands in vivo.
Journal of Investigative Dermatology, 126 (6), .
(doi:10.1038/sj.jid.5700197).
Abstract
Previous studies of the pharmacological regulation of sweat gland function in humans have administered agonists or antagonists systemically, by local intradermal injection or by iontophoresis. This has not allowed prolonged or steady-state activation of sweat glands to be examined. In this study, we used the technique of dermal microdialysis to administer pharmacological agents singly and in combination for up to 5 hours. Muscarinic stimulation with pilocarpine nitrate (50 g ml-1 to 1.66 mg ml-1) produced a sigmoid dose response curve, with maximal sweating (measured as transepidermal water loss) (mean 70 g m-2 hour-1) after 15 minutes.
This was sustained at steady-state levels (55 g m-2 hour-1) until perfusion stopped. Perfusion with atropine (0.003 mg ml-1) reduced sweating below baseline and blocked pilocarpine-induced sweating completely. Noradrenaline (0.005 mg ml-1) induced much lower sweat rates than pilocarpine (56.81.62 g m-2 hour-1 vs 8.21.2 g m-2 hour-1, respectively, P<0.001) and this was unaffected by co-administration of atropine. This method has made it possible to show that sweat glands are capable of sustaining near maximal activity for at least 5 hours. The method has future application in investigation of conditions with disordered sweat gland activity.
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Published date: 2006
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Original Article
Keywords:
tewl, transepidermal water loss
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Local EPrints ID: 27279
URI: http://eprints.soton.ac.uk/id/eprint/27279
ISSN: 0022-202X
PURE UUID: 84772df9-1689-4f63-a192-d25c43a81ce9
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Date deposited: 25 Apr 2006
Last modified: 16 Mar 2024 02:54
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Author:
Caroline J. Morgan
Author:
Peter S. Friedmann
Author:
Martin K. Church
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