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Cutaneous microdialysis as a novel means of continuously stimulating eccrine sweat glands in vivo

Record type: Article

Previous studies of the pharmacological regulation of sweat gland function in humans have administered agonists or antagonists systemically, by local intradermal injection or by iontophoresis. This has not allowed prolonged or steady-state activation of sweat glands to be examined. In this study, we used the technique of dermal microdialysis to administer pharmacological agents singly and in combination for up to 5 hours. Muscarinic stimulation with pilocarpine nitrate (50 g ml-1 to 1.66 mg ml-1) produced a sigmoid dose response curve, with maximal sweating (measured as transepidermal water loss) (mean 70 g m-2 hour-1) after 15 minutes.
This was sustained at steady-state levels (55 g m-2 hour-1) until perfusion stopped. Perfusion with atropine (0.003 mg ml-1) reduced sweating below baseline and blocked pilocarpine-induced sweating completely. Noradrenaline (0.005 mg ml-1) induced much lower sweat rates than pilocarpine (56.81.62 g m-2 hour-1 vs 8.21.2 g m-2 hour-1, respectively, P<0.001) and this was unaffected by co-administration of atropine. This method has made it possible to show that sweat glands are capable of sustaining near maximal activity for at least 5 hours. The method has future application in investigation of conditions with disordered sweat gland activity.

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Citation

Morgan, Caroline J., Friedmann, Peter S., Church, Martin K. and Clough, Geraldine F. (2006) Cutaneous microdialysis as a novel means of continuously stimulating eccrine sweat glands in vivo Journal of Investigative Dermatology, 126, (6), pp. 1220-1225. (doi:10.1038/sj.jid.5700197).

More information

Published date: 2006
Additional Information: Original Article
Keywords: tewl, transepidermal water loss

Identifiers

Local EPrints ID: 27279
URI: http://eprints.soton.ac.uk/id/eprint/27279
ISSN: 0022-202X
PURE UUID: 84772df9-1689-4f63-a192-d25c43a81ce9
ORCID for Geraldine F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

Catalogue record

Date deposited: 25 Apr 2006
Last modified: 17 Jul 2017 16:05

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Contributors

Author: Caroline J. Morgan
Author: Peter S. Friedmann
Author: Martin K. Church

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