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Expression of ErbB receptors and mucins in the airways of long term current smokers

Expression of ErbB receptors and mucins in the airways of long term current smokers
Expression of ErbB receptors and mucins in the airways of long term current smokers
Background: Airway epithelial goblet cell hyperplasia is known to occur in chronic smokers. Although the epidermal growth factor receptor has been implicated in this process, neither ErbB receptor expression nor the mucosecretory phenotype of the epithelium have been characterised in current smokers.

Methods: Bronchial biopsies obtained from non-smokers (n = 10) and current smokers, with or without chronic obstructive pulmonary disease (n = 51), were examined immunohistochemically to measure the expression of epidermal growth factor receptor, ErbB2, ErbB3, ErbB4 and mucin subtypes (MUC2, MUC5AC and MUC5B) in the bronchial epithelium. The results were correlated with neutrophil counts measured in the airway wall and induced sputum.

Results: Epidermal growth factor receptor, ErbB3 and MUC5AC expression, in addition to PAS staining, were significantly increased in all smokers compared with non-smokers, irrespective of the presence of chronic obstructive pulmonary disease. MUC5AC expression was significantly associated with both PAS staining and ErbB3 expression; no correlation was observed between either mucin or ErbB receptor expression and neutrophil counts.

Conclusions: The results suggest that long term current smoking induces enhanced epidermal growth factor receptor, ErbB3, and MUC5AC expression in vivo; these increases are not associated with the presence of neutrophilic inflammation. ErbB receptors may contribute to epithelial responses to cigarette smoke.
smoking, epithelium, mucin, growth factor receptors, chronic obstructive pulmonary disease
0040-6376
1032-1040
O'Donnell, R.A.
6202b67a-40bd-4272-af85-d174c65eeb9d
Richter, A.
080ff20b-2ec5-4204-a809-2d1c63e4be1f
Ward, J.
a8107b98-069d-4263-bd70-d29d79f07edc
Angco, G.
326db940-7378-4534-8f8e-2a1b64fc8c19
Mehta, A.
d1ffa5c9-13f5-4917-ad89-df743b516d14
Rousseau, K.
08b2d289-99f8-465f-a519-a323fec4e858
Swallow, D.M.
cbfeaf2b-d10a-4f17-ae81-65c1160434f9
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Djukanovic, R.
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8
O'Donnell, R.A.
6202b67a-40bd-4272-af85-d174c65eeb9d
Richter, A.
080ff20b-2ec5-4204-a809-2d1c63e4be1f
Ward, J.
a8107b98-069d-4263-bd70-d29d79f07edc
Angco, G.
326db940-7378-4534-8f8e-2a1b64fc8c19
Mehta, A.
d1ffa5c9-13f5-4917-ad89-df743b516d14
Rousseau, K.
08b2d289-99f8-465f-a519-a323fec4e858
Swallow, D.M.
cbfeaf2b-d10a-4f17-ae81-65c1160434f9
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Djukanovic, R.
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8

O'Donnell, R.A., Richter, A., Ward, J., Angco, G., Mehta, A., Rousseau, K., Swallow, D.M., Holgate, S.T., Djukanovic, R., Davies, D.E. and Wilson, S.J. (2004) Expression of ErbB receptors and mucins in the airways of long term current smokers. Thorax, 59 (12), 1032-1040. (doi:10.1136/thx.2004.028043). (PMID:15563701)

Record type: Article

Abstract

Background: Airway epithelial goblet cell hyperplasia is known to occur in chronic smokers. Although the epidermal growth factor receptor has been implicated in this process, neither ErbB receptor expression nor the mucosecretory phenotype of the epithelium have been characterised in current smokers.

Methods: Bronchial biopsies obtained from non-smokers (n = 10) and current smokers, with or without chronic obstructive pulmonary disease (n = 51), were examined immunohistochemically to measure the expression of epidermal growth factor receptor, ErbB2, ErbB3, ErbB4 and mucin subtypes (MUC2, MUC5AC and MUC5B) in the bronchial epithelium. The results were correlated with neutrophil counts measured in the airway wall and induced sputum.

Results: Epidermal growth factor receptor, ErbB3 and MUC5AC expression, in addition to PAS staining, were significantly increased in all smokers compared with non-smokers, irrespective of the presence of chronic obstructive pulmonary disease. MUC5AC expression was significantly associated with both PAS staining and ErbB3 expression; no correlation was observed between either mucin or ErbB receptor expression and neutrophil counts.

Conclusions: The results suggest that long term current smoking induces enhanced epidermal growth factor receptor, ErbB3, and MUC5AC expression in vivo; these increases are not associated with the presence of neutrophilic inflammation. ErbB receptors may contribute to epithelial responses to cigarette smoke.

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More information

Published date: December 2004
Keywords: smoking, epithelium, mucin, growth factor receptors, chronic obstructive pulmonary disease

Identifiers

Local EPrints ID: 27294
URI: https://eprints.soton.ac.uk/id/eprint/27294
ISSN: 0040-6376
PURE UUID: 55b6aa4a-47cc-474b-b009-30a13c5930dc
ORCID for R. Djukanovic: ORCID iD orcid.org/0000-0001-6039-5612

Catalogue record

Date deposited: 26 Apr 2006
Last modified: 22 Jun 2018 00:37

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