Pathmanathan, S., Krishna, M.T., Blomberg, A., Helleday, R., Kelly, F.J., Sandström, T., Holgate, S.T., Wilson, S.J. and Frew, A.J. (2003) Repeated daily exposure to 2 ppm nitrogen dioxide upregulates the expression of IL-5, IL-10, IL-13, and ICAM-1 in the bronchial epithelium of healthy human airways. Occupational & Environmental Medicine, 60 (11), 892-896. (doi:10.1136/oem.60.11.892).
Abstract
Background: Repeated daily exposure of healthy human subjects to NO2 induces an acute airway inflammatory response characterised by neutrophil influx in the bronchial mucosa
Aims: To assess the expression of NF-{kappa}B, cytokines, and ICAM-1 in the bronchial epithelium.
Methods: Twelve healthy, young non-smoking volunteers were exposed to 2 ppm of NO2/filtered air (four hours/day) for four successive days on separate occasions. Fibreoptic bronchoscopy was performed one hour after air and final NO2 exposures. Bronchial biopsy specimens were immunostained for NF-{kappa}B, TNF-{alpha}, eotaxin, Gro-{alpha}, GM-CSF, IL-5, -6, -8, -10, -13, and ICAM-1 and their expression was quantified using computerised image analysis.
Results: Expression of IL-5, IL-10, IL-13, and ICAM-1 increased following NO2 exposure.
Conclusion: Upregulation of the Th2 cytokines suggests that repeated exposure to NO2 has the potential to exert a "pro-allergic" effect on the bronchial epithelium. Upregulation of ICAM-1 highlights an underlying mechanism for leucocyte influx, and could also explain the predisposition to respiratory tract viral infections following NO2 exposure since ICAM-1 is a major receptor for rhino and respiratory syncytial viruses.
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