A comparison of the molecular species compositions of mammalian lung surfactant phospholipids
A comparison of the molecular species compositions of mammalian lung surfactant phospholipids
While dipalmitoyl phosphatidylcholine (PC16:0/16:0) is essential for pulmonary surfactant function, roles for other individual molecular species of surfactant phospholipids have not been established. If any phospholipid species other than PC16:0/16:0 is important for surfactant function, then it may be conserved across animal species. Consequently, we have quantified, by electrospray ionisation mass spectrometry, molecular species compositions of phosphatidylcholine (PC), phosphatidylglycerol (PG) and phosphatidylinositol (PI) in surfactants from human, rabbit, rat and guinea pig lungs. While PC compositions displayed only relatively minor variations across the animal species studied, there were wide variations of PG and PI concentrations and compositions. Human surfactant PG and PI were enriched in the same three monounsaturated species (PG16:0/18:1, PG18:1/18:1 and PG18:0/18:1) with minimal amounts of PG16:0/16:0 or polyunsaturated species, while all animal surfactant PG contained increased concentrations of PG16:0/16:0 and PG16:0/18:2. Animal surfactant PIs were essentially monounsaturated except for a high content of PI18:0/20:4 (29%) in the rat. As these four surfactants all maintain appropriate lung function of the respective animal species, then all their varied compositions of acidic phospholipids must be adequate at promoting the processes of adsorption, film refinement, respreading and collapse characteristic of surfactant. We conclude that this effectively monounsaturated composition of anionic phospholipid molecular species is a common characteristic of mammalian surfactants.
lung surfactant, human, rat, rabbit, guinea pig, electrospray ionisation mass spectrometry, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol
65-73
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Heeley, Emma L.
a419a91d-b809-4dc6-af94-40052cb5be82
Wilton, David C.
4b995f66-ad6c-4d96-9179-c64f3b54466a
2001
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Heeley, Emma L.
a419a91d-b809-4dc6-af94-40052cb5be82
Wilton, David C.
4b995f66-ad6c-4d96-9179-c64f3b54466a
Postle, Anthony D., Heeley, Emma L. and Wilton, David C.
(2001)
A comparison of the molecular species compositions of mammalian lung surfactant phospholipids.
Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology, 129 (1), .
(doi:10.1016/S1095-6433(01)00306-3).
Abstract
While dipalmitoyl phosphatidylcholine (PC16:0/16:0) is essential for pulmonary surfactant function, roles for other individual molecular species of surfactant phospholipids have not been established. If any phospholipid species other than PC16:0/16:0 is important for surfactant function, then it may be conserved across animal species. Consequently, we have quantified, by electrospray ionisation mass spectrometry, molecular species compositions of phosphatidylcholine (PC), phosphatidylglycerol (PG) and phosphatidylinositol (PI) in surfactants from human, rabbit, rat and guinea pig lungs. While PC compositions displayed only relatively minor variations across the animal species studied, there were wide variations of PG and PI concentrations and compositions. Human surfactant PG and PI were enriched in the same three monounsaturated species (PG16:0/18:1, PG18:1/18:1 and PG18:0/18:1) with minimal amounts of PG16:0/16:0 or polyunsaturated species, while all animal surfactant PG contained increased concentrations of PG16:0/16:0 and PG16:0/18:2. Animal surfactant PIs were essentially monounsaturated except for a high content of PI18:0/20:4 (29%) in the rat. As these four surfactants all maintain appropriate lung function of the respective animal species, then all their varied compositions of acidic phospholipids must be adequate at promoting the processes of adsorption, film refinement, respreading and collapse characteristic of surfactant. We conclude that this effectively monounsaturated composition of anionic phospholipid molecular species is a common characteristic of mammalian surfactants.
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Published date: 2001
Keywords:
lung surfactant, human, rat, rabbit, guinea pig, electrospray ionisation mass spectrometry, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol
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Local EPrints ID: 27334
URI: http://eprints.soton.ac.uk/id/eprint/27334
ISSN: 1095-6433
PURE UUID: 49150a3b-91a2-49c0-825f-8e9d4b0f4058
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Date deposited: 26 Apr 2006
Last modified: 16 Mar 2024 02:32
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Author:
Emma L. Heeley
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