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Persistent airway T-lymphocyte activation in chronic corticosteroid-treated symptomatic asthma

Persistent airway T-lymphocyte activation in chronic corticosteroid-treated symptomatic asthma
Persistent airway T-lymphocyte activation in chronic corticosteroid-treated symptomatic asthma
Background: A small proportion of patients with asthma have persistent symptoms despite regular treatment with high-dose inhaled and/or oral corticosteroids. There is little information regarding immunopathology in such patients.
Objective: To compare airway inflammatory changes in subjects with chronic corticosteroid-dependent symptomatic asthma (n = 5) and subjects with asthma that was clinically well controlled on inhaled corticosteroid therapy (n = 9). Subjects in the corticosteroid-dependent group were receiving long-term treatment with oral prednisolone and high-dose inhaled corticosteroids.
Methods: Subjects underwent fiberoptic bronchoscopy with bronchoalveolar lavage (BAL) and bronchial biopsy. T-lymphocytes subsets and activation markers in BAL fluid and peripheral blood were determined by FACS analysis. Bronchial biopsies were stained immunohistochemically, and numbers of inflammatory cells quantitated. Inflammatory mediators in BAL fluid were measured by immunoassay.
Results: There was significantly greater expression of CD25 (P = .02) and HLA-DR (P = .04) by BAL fluid T-lymphocytes in corticosteroid-treated symptomatic asthmatics. In bronchial biopsies there were no significant differences between the two groups in the numbers of AA1+ cells (mast cells), EG2+ cells (eosinophils) or MT1+ T-lymphocytes. Levels of albumin, histamine, tryptase, and eosinophil cationic protein in BAL fluid did not differ significantly between groups.
Conclusions: Chronic corticosteroid-treated symptomatic asthma is associated with persistent airway T-lymphocyte activation. This, however, is not necessarily accompanied by the recruitment and activation of inflammatory cells within the airways.
1081-1206
501-507
Redington, Anthony E.
d61be7d5-850e-4d5c-a078-5603af44371e
Wilson, John W.
ac7d2c8f-67d7-4ee7-a196-e980caf8d226
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Madden, Jacqueline
0771e352-d432-41ea-8a7e-4704c1efca46
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Howarth, Peter H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Redington, Anthony E.
d61be7d5-850e-4d5c-a078-5603af44371e
Wilson, John W.
ac7d2c8f-67d7-4ee7-a196-e980caf8d226
Walls, Andrew F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Madden, Jacqueline
0771e352-d432-41ea-8a7e-4704c1efca46
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Howarth, Peter H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21

Redington, Anthony E., Wilson, John W., Walls, Andrew F., Madden, Jacqueline, Djukanovic, Ratko, Holgate, Stephen T. and Howarth, Peter H. (2000) Persistent airway T-lymphocyte activation in chronic corticosteroid-treated symptomatic asthma. Annals of Allergy, Asthma and Immunology, 85 (6), 501-507.

Record type: Article

Abstract

Background: A small proportion of patients with asthma have persistent symptoms despite regular treatment with high-dose inhaled and/or oral corticosteroids. There is little information regarding immunopathology in such patients.
Objective: To compare airway inflammatory changes in subjects with chronic corticosteroid-dependent symptomatic asthma (n = 5) and subjects with asthma that was clinically well controlled on inhaled corticosteroid therapy (n = 9). Subjects in the corticosteroid-dependent group were receiving long-term treatment with oral prednisolone and high-dose inhaled corticosteroids.
Methods: Subjects underwent fiberoptic bronchoscopy with bronchoalveolar lavage (BAL) and bronchial biopsy. T-lymphocytes subsets and activation markers in BAL fluid and peripheral blood were determined by FACS analysis. Bronchial biopsies were stained immunohistochemically, and numbers of inflammatory cells quantitated. Inflammatory mediators in BAL fluid were measured by immunoassay.
Results: There was significantly greater expression of CD25 (P = .02) and HLA-DR (P = .04) by BAL fluid T-lymphocytes in corticosteroid-treated symptomatic asthmatics. In bronchial biopsies there were no significant differences between the two groups in the numbers of AA1+ cells (mast cells), EG2+ cells (eosinophils) or MT1+ T-lymphocytes. Levels of albumin, histamine, tryptase, and eosinophil cationic protein in BAL fluid did not differ significantly between groups.
Conclusions: Chronic corticosteroid-treated symptomatic asthma is associated with persistent airway T-lymphocyte activation. This, however, is not necessarily accompanied by the recruitment and activation of inflammatory cells within the airways.

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More information

Published date: 2000
Organisations: Infection Inflammation & Immunity, Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 27348
URI: http://eprints.soton.ac.uk/id/eprint/27348
ISSN: 1081-1206
PURE UUID: c9c45dd4-8cf2-434a-989b-2be8c80a6230
ORCID for Andrew F. Walls: ORCID iD orcid.org/0000-0003-4803-4595
ORCID for Ratko Djukanovic: ORCID iD orcid.org/0000-0001-6039-5612

Catalogue record

Date deposited: 27 Apr 2006
Last modified: 03 Aug 2022 01:33

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Contributors

Author: Anthony E. Redington
Author: John W. Wilson
Author: Andrew F. Walls ORCID iD
Author: Jacqueline Madden

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