Equivalent effects of snake PLA2 neurotoxins and lysophospholipid-fatty acid mixtures
Equivalent effects of snake PLA2 neurotoxins and lysophospholipid-fatty acid mixtures
Snake presynaptic phospholipase A2 neurotoxins (SPANs) paralyze the neuromuscular junction (NMJ). Upon intoxication, the NMJ enlarges and has a reduced content of synaptic vesicles, and primary neuronal cultures show synaptic swelling with surface exposure of the lumenal domain of the synaptic vesicle protein synaptotagmin I. Concomitantly, these neurotoxins induce exocytosis of neurotransmitters. We found that an equimolar mixture of lysophospholipids and fatty acids closely mimics all of the biological effects of SPANs. These results draw attention to the possible role of local lipid changes in synaptic vesicle release and provide new tools for the study of exocytosis.
1678-1680
Rigoni, Michela
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Caccin, Paola
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Gschmeissner, Steve
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Koster, Grielof
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Postle, Anthony D.
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Rossetto, Ornella
01dad575-df44-445d-b481-a0eb9352bb6f
Schiavo, Giampietro
0e309b58-ff46-4c10-8eb5-42b380b24223
Montecucco, Cesare
3041113c-40d5-4998-8a33-cd9693909f18
2005
Rigoni, Michela
f9adfe5a-3246-481c-b5e6-ccf5e6f45d66
Caccin, Paola
bddec3a5-f096-484e-8023-988f95a7137e
Gschmeissner, Steve
080011a7-fa52-4417-8b0e-327a49f2b2d8
Koster, Grielof
369252d2-1126-4fa6-bad1-bcd6983ca8b7
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Rossetto, Ornella
01dad575-df44-445d-b481-a0eb9352bb6f
Schiavo, Giampietro
0e309b58-ff46-4c10-8eb5-42b380b24223
Montecucco, Cesare
3041113c-40d5-4998-8a33-cd9693909f18
Rigoni, Michela, Caccin, Paola, Gschmeissner, Steve, Koster, Grielof, Postle, Anthony D., Rossetto, Ornella, Schiavo, Giampietro and Montecucco, Cesare
(2005)
Equivalent effects of snake PLA2 neurotoxins and lysophospholipid-fatty acid mixtures.
Science, 310 (5754), .
(doi:10.1126/science.1120640).
Abstract
Snake presynaptic phospholipase A2 neurotoxins (SPANs) paralyze the neuromuscular junction (NMJ). Upon intoxication, the NMJ enlarges and has a reduced content of synaptic vesicles, and primary neuronal cultures show synaptic swelling with surface exposure of the lumenal domain of the synaptic vesicle protein synaptotagmin I. Concomitantly, these neurotoxins induce exocytosis of neurotransmitters. We found that an equimolar mixture of lysophospholipids and fatty acids closely mimics all of the biological effects of SPANs. These results draw attention to the possible role of local lipid changes in synaptic vesicle release and provide new tools for the study of exocytosis.
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Published date: 2005
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Local EPrints ID: 27369
URI: http://eprints.soton.ac.uk/id/eprint/27369
ISSN: 0036-8075
PURE UUID: a660e515-2510-464a-bd50-df9223793df9
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Date deposited: 27 Apr 2006
Last modified: 16 Mar 2024 02:32
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Author:
Michela Rigoni
Author:
Paola Caccin
Author:
Steve Gschmeissner
Author:
Grielof Koster
Author:
Ornella Rossetto
Author:
Giampietro Schiavo
Author:
Cesare Montecucco
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