Recognition of pollen-derived phosphatidyl-ethanolamine by human CD1d-restricted ?? T cells
Recognition of pollen-derived phosphatidyl-ethanolamine by human CD1d-restricted ?? T cells
Background: Evidences from mice and human beings indicate that ?? T cells could be relevant in recognition of stress-induced self and/or yet unidentified inhaled foreign antigens. Their specificity differs from classic MHC-restricted ?? T cells and involves the immunoglobulin-like structure of the ?? T-cell receptor with the recognition of small organic molecules, alkylamines, and self lipid compounds presented by CD1+ dendritic cells.
Objective: Because CD1 receptors are mainly devoted to lipid antigen presentation, we sought to determine whether exogenous pollen membrane lipids may act as allergens for CD1-restricted ?? T cells.
Methods: Peripheral blood and nasal mucosa-associated ?? T cells were cloned from normal controls and cypress-sensitive subjects and tested for their antigen specificity and CD1-restriction with phospholipids extracted from tree pollen grains, as well with other natural or synthetic compounds. Phospholipid reactivity of cloned ?? T cells was measured by mean of proliferative response and cytokine release as well as by testing their helper activity on IgE production in vitro and in vivo.
Results: Cloned ?? T lymphocytes from subjects with allergy, but not normal controls, were found to recognize pollen-derived phosphatidyl-ethanolamine (PE) in a CD1d-restricted fashion. Only 16:0/18:2 and 18:2/18:2 PE were stimulatory, whereas no response was recorded for disaturated PE, phosphatidylcholine, neutral lipids, or protein extract. Proliferating clones secreted both TH1-type and TH2-type cytokines and drove IgE production in vitro and in vivo.
Conclusion: CD1d-restricted ?? T cells specific for phospholipids can represent a key mucosal regulatory subset for the control of early host reactivity against tree pollens.
Clinical implications: By knowing how lipid allergen constituents interact with mucosal immune system, we can expand our possibilities in diagnostic and therapeutic interventions.
cypress pollen, olive pollen, phospholipids, phosphatidyl-ethanolamine, rhinitis, nasal mucosa, ?? t lymphocytes, t-cell clones, cd1-restriction, cytokines, il-4, skin prick tests, specific ige
1178-1184
Russano, Anna M.
b77fec61-e3f5-40e9-afe6-d678e39b44c0
Agea, Elisabetta
c9537ce6-7cc1-4d03-9449-31f770efe298
Corazzi, Lanfranco
2353df1c-ec76-4986-8c18-25ab8006819b
Postle, Antyony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
De Libero, Gennaro
00d26d61-d193-448f-b291-369e51101136
Porcelli, Steven
a39a5f4c-5fac-47b3-9507-be28e3f94764
de Benedictis, Fernando M.
746ce355-a43f-4fd9-8e47-e908423491a4
Spinozzi, Fabrizio
515ccc52-98d5-4e1c-9d21-8d777c7651cd
2006
Russano, Anna M.
b77fec61-e3f5-40e9-afe6-d678e39b44c0
Agea, Elisabetta
c9537ce6-7cc1-4d03-9449-31f770efe298
Corazzi, Lanfranco
2353df1c-ec76-4986-8c18-25ab8006819b
Postle, Antyony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
De Libero, Gennaro
00d26d61-d193-448f-b291-369e51101136
Porcelli, Steven
a39a5f4c-5fac-47b3-9507-be28e3f94764
de Benedictis, Fernando M.
746ce355-a43f-4fd9-8e47-e908423491a4
Spinozzi, Fabrizio
515ccc52-98d5-4e1c-9d21-8d777c7651cd
Russano, Anna M., Agea, Elisabetta, Corazzi, Lanfranco, Postle, Antyony D., De Libero, Gennaro, Porcelli, Steven, de Benedictis, Fernando M. and Spinozzi, Fabrizio
(2006)
Recognition of pollen-derived phosphatidyl-ethanolamine by human CD1d-restricted ?? T cells.
Journal of Allergy and Clinical Immunology, 117 (5), .
(doi:10.1016/j.jaci.2006.01.001).
Abstract
Background: Evidences from mice and human beings indicate that ?? T cells could be relevant in recognition of stress-induced self and/or yet unidentified inhaled foreign antigens. Their specificity differs from classic MHC-restricted ?? T cells and involves the immunoglobulin-like structure of the ?? T-cell receptor with the recognition of small organic molecules, alkylamines, and self lipid compounds presented by CD1+ dendritic cells.
Objective: Because CD1 receptors are mainly devoted to lipid antigen presentation, we sought to determine whether exogenous pollen membrane lipids may act as allergens for CD1-restricted ?? T cells.
Methods: Peripheral blood and nasal mucosa-associated ?? T cells were cloned from normal controls and cypress-sensitive subjects and tested for their antigen specificity and CD1-restriction with phospholipids extracted from tree pollen grains, as well with other natural or synthetic compounds. Phospholipid reactivity of cloned ?? T cells was measured by mean of proliferative response and cytokine release as well as by testing their helper activity on IgE production in vitro and in vivo.
Results: Cloned ?? T lymphocytes from subjects with allergy, but not normal controls, were found to recognize pollen-derived phosphatidyl-ethanolamine (PE) in a CD1d-restricted fashion. Only 16:0/18:2 and 18:2/18:2 PE were stimulatory, whereas no response was recorded for disaturated PE, phosphatidylcholine, neutral lipids, or protein extract. Proliferating clones secreted both TH1-type and TH2-type cytokines and drove IgE production in vitro and in vivo.
Conclusion: CD1d-restricted ?? T cells specific for phospholipids can represent a key mucosal regulatory subset for the control of early host reactivity against tree pollens.
Clinical implications: By knowing how lipid allergen constituents interact with mucosal immune system, we can expand our possibilities in diagnostic and therapeutic interventions.
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More information
Submitted date: July 2005
Published date: 2006
Additional Information:
Original article (corrected proof): Received 2 July 2005; revised 2 January 2006; accepted 4 January 2006. Perugia and Ancona, Italy, Southampton, United Kingdom, Basel, Switzerland, and Bronx, NY. Available online 21 February 2006.
Keywords:
cypress pollen, olive pollen, phospholipids, phosphatidyl-ethanolamine, rhinitis, nasal mucosa, ?? t lymphocytes, t-cell clones, cd1-restriction, cytokines, il-4, skin prick tests, specific ige
Identifiers
Local EPrints ID: 27396
URI: http://eprints.soton.ac.uk/id/eprint/27396
ISSN: 0091-6749
PURE UUID: f7d907a0-fc83-4a26-8e77-48a8631d7677
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Date deposited: 27 Apr 2006
Last modified: 16 Mar 2024 02:32
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Contributors
Author:
Anna M. Russano
Author:
Elisabetta Agea
Author:
Lanfranco Corazzi
Author:
Gennaro De Libero
Author:
Steven Porcelli
Author:
Fernando M. de Benedictis
Author:
Fabrizio Spinozzi
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