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Nasal mucosal immunoexpression of the mast cell chemoattractants TGF-β, eotaxin, and stem cell factor and their receptors in allergic rhinitis

Nasal mucosal immunoexpression of the mast cell chemoattractants TGF-β, eotaxin, and stem cell factor and their receptors in allergic rhinitis
Nasal mucosal immunoexpression of the mast cell chemoattractants TGF-β, eotaxin, and stem cell factor and their receptors in allergic rhinitis
Background

Allergic rhinitis is characterized by the epithelial accumulation of cells, particularly mast cells and eosinophils. There is little information relating to the chemotaxins responsible for mast cell epithelial accumulation in this disease.

Objective

Expression of the mast cell chemoattractants TGF-β, eotaxin, and stem cell factor and their receptors was investigated in tissue sections from biopsy specimens obtained from patients with naturally occurring allergic rhinitis.

Methods

Specific immunohistochemical staining was performed on thin sections of inferior turbinate biopsy specimens from patients with perennial and seasonal allergic rhinitis and, for comparison, from nonatopic and, where relevant, atopic healthy volunteers without rhinitis. Sequential staining of adjacent 2-μm sections was undertaken to colocalize TGF-β receptors to mast cells.

Results

Evidence was found of significantly increased epithelial immunoreactivity for TGF-β1, TGF-β2, TGF-β3, TGF-β receptor I, TGF-β receptor II, and TGF-β receptor III in patients with perennial and seasonal allergic rhinitis compared with that seen in healthy control subjects. TGF-β receptors I and II were found to colocalize to mast cells. Eotaxin epithelial immunoreactivity was significantly increased in the perennial group, although there were no corresponding disease-related differences found in relation to CCR-3 immunoreactivity at this site. There was no increase in stem cell factor immunoreactivity within the epithelium in naturally occurring disease. Significant correlations were found between epithelial immunoreactivity for TGF-β1, TGF-β2, TGF-β receptor I, TGF-β receptor II, and the number of epithelial mast cells.

Conclusion

These findings of enhanced epithelial TGF-β immunoreactivity in patients with rhinitis, the correlation with intraepithelial mast cell numbers, and the colocalization of TGF-β receptors to mast cells suggest that the epithelial expression of TGF-β might represent an important biologic process involved in either the recruitment or retention of mast cells within the epithelium in naturally occurring allergic rhinitis.
allergic rhinitis, immunohistochemistry, human, mast cells, antibodies, allergy, chemotaxis, tgf-?, eotaxin, stem cell factor
0091-6749
799-806
Salib, Rami J.
d6fde1c1-5b5e-43f7-ae1c-42cce6a0c9fc
Kumar, Sanjiv
4488e829-d6dc-45e3-b6ef-a746e94bcb96
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Howarth, Peter H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Salib, Rami J.
d6fde1c1-5b5e-43f7-ae1c-42cce6a0c9fc
Kumar, Sanjiv
4488e829-d6dc-45e3-b6ef-a746e94bcb96
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Howarth, Peter H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21

Salib, Rami J., Kumar, Sanjiv, Wilson, Susan J. and Howarth, Peter H. (2004) Nasal mucosal immunoexpression of the mast cell chemoattractants TGF-β, eotaxin, and stem cell factor and their receptors in allergic rhinitis. Journal of Allergy and Clinical Immunology, 114 (4), 799-806. (doi:10.1016/j.jaci.2004.07.010).

Record type: Article

Abstract

Background

Allergic rhinitis is characterized by the epithelial accumulation of cells, particularly mast cells and eosinophils. There is little information relating to the chemotaxins responsible for mast cell epithelial accumulation in this disease.

Objective

Expression of the mast cell chemoattractants TGF-β, eotaxin, and stem cell factor and their receptors was investigated in tissue sections from biopsy specimens obtained from patients with naturally occurring allergic rhinitis.

Methods

Specific immunohistochemical staining was performed on thin sections of inferior turbinate biopsy specimens from patients with perennial and seasonal allergic rhinitis and, for comparison, from nonatopic and, where relevant, atopic healthy volunteers without rhinitis. Sequential staining of adjacent 2-μm sections was undertaken to colocalize TGF-β receptors to mast cells.

Results

Evidence was found of significantly increased epithelial immunoreactivity for TGF-β1, TGF-β2, TGF-β3, TGF-β receptor I, TGF-β receptor II, and TGF-β receptor III in patients with perennial and seasonal allergic rhinitis compared with that seen in healthy control subjects. TGF-β receptors I and II were found to colocalize to mast cells. Eotaxin epithelial immunoreactivity was significantly increased in the perennial group, although there were no corresponding disease-related differences found in relation to CCR-3 immunoreactivity at this site. There was no increase in stem cell factor immunoreactivity within the epithelium in naturally occurring disease. Significant correlations were found between epithelial immunoreactivity for TGF-β1, TGF-β2, TGF-β receptor I, TGF-β receptor II, and the number of epithelial mast cells.

Conclusion

These findings of enhanced epithelial TGF-β immunoreactivity in patients with rhinitis, the correlation with intraepithelial mast cell numbers, and the colocalization of TGF-β receptors to mast cells suggest that the epithelial expression of TGF-β might represent an important biologic process involved in either the recruitment or retention of mast cells within the epithelium in naturally occurring allergic rhinitis.

Full text not available from this repository.

More information

Published date: October 2004
Keywords: allergic rhinitis, immunohistochemistry, human, mast cells, antibodies, allergy, chemotaxis, tgf-?, eotaxin, stem cell factor

Identifiers

Local EPrints ID: 27400
URI: https://eprints.soton.ac.uk/id/eprint/27400
ISSN: 0091-6749
PURE UUID: ca19f515-3cf5-465d-83f2-4159ecbefa33
ORCID for Rami J. Salib: ORCID iD orcid.org/0000-0002-6753-7844

Catalogue record

Date deposited: 27 Apr 2006
Last modified: 21 Jul 2018 00:33

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