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Nasal lavage fluid concentrations of eotaxin-1 (CCL11) in naturally occurring allergic rhinitis: relationship to disease activity, nasal luminal eosinophil influx, and plasma protein exudation

Nasal lavage fluid concentrations of eotaxin-1 (CCL11) in naturally occurring allergic rhinitis: relationship to disease activity, nasal luminal eosinophil influx, and plasma protein exudation
Nasal lavage fluid concentrations of eotaxin-1 (CCL11) in naturally occurring allergic rhinitis: relationship to disease activity, nasal luminal eosinophil influx, and plasma protein exudation
Background Eotaxin-1 (CCL11) is a CC chemokine whose nasal eosinophilic chemotactic activity in vivo and in vitro has been demonstrated primarily using nasal allergen challenge models. The extension of these challenge findings to the in vivo setting has been limited.
Objective To obtain nasal lavage fluid from volunteers with perennial and seasonal (in- and out-of-season) allergic rhinitis (AR) and non-atopic non-rhinitic controls for the measurement of eotaxin-1 concentrations and to relate these findings to the symptomatic disease severity, the percentage of lavage eosinophils, and to ?2-macroglobulin (?2-MG) lavage concentrations, as a marker of vascular permeability and an index of airway inflammation.
Methods Thirty-seven volunteers with AR (16 seasonal and 21 perennial) and 20 non-atopic non-rhinitic volunteers were recruited and phenotyped. Nasal lavage fluid was obtained by standardized protocol. The nasal lavage fluid concentrations of eotaxin and ?2-MG were measured by ELISA, and differential cell counts performed on cytospins.
Results Eotaxin-1 nasal lavage fluid concentrations were significantly higher in both the perennial and seasonal (in-season) AR groups compared with the controls, and significantly related to the severity of symptom expression and to the percentage of lavage eosinophils. The lavage eosinophil counts were significantly higher in both the symptomatic rhinitis groups compared with the control groups and correlated with the lavage concentrations of ?2-MG. ?2-MG levels were significantly increased in seasonal (in-season) rhinitics compared with both non-atopic controls and seasonal (out-of-season) rhinitics. A significant correlation was observed between the levels of ?2-MG and levels of eotaxin in the symptomatic allergic rhinitic groups.
Conclusions This study clearly demonstrates the relevance of eotaxin-1 to the pathogenesis of naturally occurring AR.
0954-7894
995-1002
Salib, R.J.
962f24b6-1828-47e7-9827-4a627c63b18b
Lau, L.C.
2af8045d-6162-4939-aba7-28dd2f60f6a8
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Salib, R.J.
962f24b6-1828-47e7-9827-4a627c63b18b
Lau, L.C.
2af8045d-6162-4939-aba7-28dd2f60f6a8
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21

Salib, R.J., Lau, L.C. and Howarth, P.H. (2005) Nasal lavage fluid concentrations of eotaxin-1 (CCL11) in naturally occurring allergic rhinitis: relationship to disease activity, nasal luminal eosinophil influx, and plasma protein exudation. Clinical & Experimental Allergy, 35 (8), 995-1002. (doi:10.1111/j.1365-2222.2005.02236.x).

Record type: Article

Abstract

Background Eotaxin-1 (CCL11) is a CC chemokine whose nasal eosinophilic chemotactic activity in vivo and in vitro has been demonstrated primarily using nasal allergen challenge models. The extension of these challenge findings to the in vivo setting has been limited.
Objective To obtain nasal lavage fluid from volunteers with perennial and seasonal (in- and out-of-season) allergic rhinitis (AR) and non-atopic non-rhinitic controls for the measurement of eotaxin-1 concentrations and to relate these findings to the symptomatic disease severity, the percentage of lavage eosinophils, and to ?2-macroglobulin (?2-MG) lavage concentrations, as a marker of vascular permeability and an index of airway inflammation.
Methods Thirty-seven volunteers with AR (16 seasonal and 21 perennial) and 20 non-atopic non-rhinitic volunteers were recruited and phenotyped. Nasal lavage fluid was obtained by standardized protocol. The nasal lavage fluid concentrations of eotaxin and ?2-MG were measured by ELISA, and differential cell counts performed on cytospins.
Results Eotaxin-1 nasal lavage fluid concentrations were significantly higher in both the perennial and seasonal (in-season) AR groups compared with the controls, and significantly related to the severity of symptom expression and to the percentage of lavage eosinophils. The lavage eosinophil counts were significantly higher in both the symptomatic rhinitis groups compared with the control groups and correlated with the lavage concentrations of ?2-MG. ?2-MG levels were significantly increased in seasonal (in-season) rhinitics compared with both non-atopic controls and seasonal (out-of-season) rhinitics. A significant correlation was observed between the levels of ?2-MG and levels of eotaxin in the symptomatic allergic rhinitic groups.
Conclusions This study clearly demonstrates the relevance of eotaxin-1 to the pathogenesis of naturally occurring AR.

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Published date: 2005

Identifiers

Local EPrints ID: 27401
URI: http://eprints.soton.ac.uk/id/eprint/27401
ISSN: 0954-7894
PURE UUID: 039d9090-4e9b-4099-9faf-c71db497c3fc

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Date deposited: 25 Apr 2006
Last modified: 15 Mar 2024 07:18

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Contributors

Author: R.J. Salib
Author: L.C. Lau
Author: P.H. Howarth

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