n-3 polyunsaturated fatty acid supplementation, monocyte adhesion molecule expression and pro-inflammatory mediators in Type 2 diabetes mellitus
n-3 polyunsaturated fatty acid supplementation, monocyte adhesion molecule expression and pro-inflammatory mediators in Type 2 diabetes mellitus
Aims: To examine the effect of n-3 polyunsaturated fatty acid supplements on the monocyte surface expression of adhesion molecules involved in pro-atherogenic monocyte–endothelial interactions, and on pro-inflammatory mediators in Type 2 diabetes mellitus.
Methods: Twenty-nine subjects with Type 2 diabetes and 21 controls without diabetes were studied. Monocyte expression of leucocyte function-associated antigens 1 and 3, intercellular adhesion molecule-1, and the major histocompatibility complex class II molecule HLA-DR were measured using a laser flow cytometric method. Supplementation with 2.08 g n-3 fatty acids for 21 days was undertaken and measurements repeated. Plasma soluble adhesion molecule concentrations, plasminogen activator inhibitor-1 activity and antigen and pro-inflammatory mediators (cysteinyl leukotriene and monocyte leukotriene B4) were also measured.
Results: Groups did not differ in monocyte expression of adhesion molecules or HLA-DR, or in leukotriene production although plasma soluble adhesion molecule concentrations were higher in the diabetes groups (P < 0.05). n-3 fatty acid supplementation influenced neither the expression of these molecules nor plasma soluble adhesion molecule concentrations or leukotriene production.
Conclusions: This study does not support increased monocyte adhesion molecule expression or abnormal monocyte production of pro-inflammatory mediators as mechanisms for increased atherogenic risk in Type 2 diabetes. Cardioprotective actions of n-3 fatty acids may not be mediated through these mechanisms.
51-58
Sampson, M.J.
404dc10f-4f0b-4396-b866-cb99ce25c650
Davies, I.R.
d14a5aa0-bff7-41ae-8e4a-d1ab54669fef
Brown, J.C.
a99cc54c-a1f6-46ef-949d-c9d1c20596fa
Morgan, V.
236311a0-7ee9-41d1-88d1-b894d4487b78
Richardson, T.
1c289348-af0f-4828-bf90-f241825c13d1
James, A.J.
f2040b75-5c66-49ba-a2e5-bc211cfdfcda
Sampson, A.P.
4ca76f6f-ff35-425d-a7e7-c2bd2ea2df60
Hughes, D.A.
69853fa6-1773-4076-8642-ce6b59ed5566
2001
Sampson, M.J.
404dc10f-4f0b-4396-b866-cb99ce25c650
Davies, I.R.
d14a5aa0-bff7-41ae-8e4a-d1ab54669fef
Brown, J.C.
a99cc54c-a1f6-46ef-949d-c9d1c20596fa
Morgan, V.
236311a0-7ee9-41d1-88d1-b894d4487b78
Richardson, T.
1c289348-af0f-4828-bf90-f241825c13d1
James, A.J.
f2040b75-5c66-49ba-a2e5-bc211cfdfcda
Sampson, A.P.
4ca76f6f-ff35-425d-a7e7-c2bd2ea2df60
Hughes, D.A.
69853fa6-1773-4076-8642-ce6b59ed5566
Sampson, M.J., Davies, I.R., Brown, J.C., Morgan, V., Richardson, T., James, A.J., Sampson, A.P. and Hughes, D.A.
(2001)
n-3 polyunsaturated fatty acid supplementation, monocyte adhesion molecule expression and pro-inflammatory mediators in Type 2 diabetes mellitus.
Diabetic Medicine, 18 (1), .
(doi:10.1046/j.1464-5491.2001.00410.x).
Abstract
Aims: To examine the effect of n-3 polyunsaturated fatty acid supplements on the monocyte surface expression of adhesion molecules involved in pro-atherogenic monocyte–endothelial interactions, and on pro-inflammatory mediators in Type 2 diabetes mellitus.
Methods: Twenty-nine subjects with Type 2 diabetes and 21 controls without diabetes were studied. Monocyte expression of leucocyte function-associated antigens 1 and 3, intercellular adhesion molecule-1, and the major histocompatibility complex class II molecule HLA-DR were measured using a laser flow cytometric method. Supplementation with 2.08 g n-3 fatty acids for 21 days was undertaken and measurements repeated. Plasma soluble adhesion molecule concentrations, plasminogen activator inhibitor-1 activity and antigen and pro-inflammatory mediators (cysteinyl leukotriene and monocyte leukotriene B4) were also measured.
Results: Groups did not differ in monocyte expression of adhesion molecules or HLA-DR, or in leukotriene production although plasma soluble adhesion molecule concentrations were higher in the diabetes groups (P < 0.05). n-3 fatty acid supplementation influenced neither the expression of these molecules nor plasma soluble adhesion molecule concentrations or leukotriene production.
Conclusions: This study does not support increased monocyte adhesion molecule expression or abnormal monocyte production of pro-inflammatory mediators as mechanisms for increased atherogenic risk in Type 2 diabetes. Cardioprotective actions of n-3 fatty acids may not be mediated through these mechanisms.
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Published date: 2001
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Local EPrints ID: 27413
URI: http://eprints.soton.ac.uk/id/eprint/27413
ISSN: 0742-3071
PURE UUID: 1fd21bd4-c2e5-4ac4-9680-3f547210d680
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Date deposited: 27 Apr 2006
Last modified: 16 Mar 2024 02:51
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Author:
M.J. Sampson
Author:
I.R. Davies
Author:
J.C. Brown
Author:
V. Morgan
Author:
T. Richardson
Author:
A.J. James
Author:
D.A. Hughes
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