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Surface expression of Fc?RI on Langerhans' cells of clinically uninvolved skin is associated with disease activity in atopic dermatitis, allergic asthma, and rhinitis

Surface expression of Fc?RI on Langerhans' cells of clinically uninvolved skin is associated with disease activity in atopic dermatitis, allergic asthma, and rhinitis
Surface expression of Fc?RI on Langerhans' cells of clinically uninvolved skin is associated with disease activity in atopic dermatitis, allergic asthma, and rhinitis
Background: Fc?RI expressed on the surface of human epidermal Langerhans' cells facilitates uptake of IgE-associated allergens and plays a pivotal role in the pathogenesis of atopic dermatitis. Seminal results from studies investigating Langerhans' cell Fc?RI in skin biopsy sections or epidermal cell suspensions demonstrate the highest receptor expression in lesional skin of patients with active atopic dermatitis.
Objective: We sought to investigate and localize Fc?RI expression on Langerhans' cells within a minimally disturbed tissue environment in clinically uninvolved skin and to compare receptor expression between healthy donors and patients with atopic dermatitis or other allergic diseases.
Methods: Intact epidermal sheets from skin suction blisters, immunofluorescently stained with Langerhans' cell markers and anti-Fc?RI? (mAbs 15E5 and 22E7) or anti-IgE, were examined by means of confocal microscopy. Samples incubated with anti-Fc?RI? before or after cell fixation-permeabilization were compared to discriminate between cytoplasmic and membrane localization.
Results: Cytoplasmic Fc?RI ? chain was found in Langerhans' cells from all donors, irrespective of atopic status. Surface Fc?RI-bound IgE was detected in the skin of individuals with active atopic dermatitis and in the skin of those with active asthma or rhinitis. No surface Fc?RI was expressed in the skin of patients with a clinical history of atopic dermatitis, asthma, or rhinitis whose disease was in remission or in the skin of nonatopic individuals.
Conclusion: In clinically uninvolved skin, Langerhans' cell-surface Fc?RI expression is not only linked to atopic dermatitis but is also generally associated with allergic disease. This supports the concept of a systemic regulatory mechanism associated with active allergic disease, which is further aggravated by local inflammation in atopic skin lesions.
0091-6749
411-419
Semper, Amanda E.
7271f3d0-051a-444d-a75f-e1d374a93bc8
Heron, Kyle
c75fdd39-b8d8-4ff6-9b3f-34f69935e780
Woollard, Alexander C.S.
5b053446-e7f6-4ec3-b548-a81c14cbd906
Kochan, Jarema P.
1ff5878b-054a-451a-ab4e-60e119158530
Friedmann, Peter S.
d50bac23-f3ec-4493-8fa0-fa126cbeba88
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Reischl, Ilona G.
b6efc2fd-04fb-4aec-b38c-b86a095c8dd4
Semper, Amanda E.
7271f3d0-051a-444d-a75f-e1d374a93bc8
Heron, Kyle
c75fdd39-b8d8-4ff6-9b3f-34f69935e780
Woollard, Alexander C.S.
5b053446-e7f6-4ec3-b548-a81c14cbd906
Kochan, Jarema P.
1ff5878b-054a-451a-ab4e-60e119158530
Friedmann, Peter S.
d50bac23-f3ec-4493-8fa0-fa126cbeba88
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Reischl, Ilona G.
b6efc2fd-04fb-4aec-b38c-b86a095c8dd4

Semper, Amanda E., Heron, Kyle, Woollard, Alexander C.S., Kochan, Jarema P., Friedmann, Peter S., Church, Martin K. and Reischl, Ilona G. (2003) Surface expression of Fc?RI on Langerhans' cells of clinically uninvolved skin is associated with disease activity in atopic dermatitis, allergic asthma, and rhinitis. Journal of Allergy and Clinical Immunology, 112 (2), 411-419. (doi:10.1067/mai.2003.1626).

Record type: Article

Abstract

Background: Fc?RI expressed on the surface of human epidermal Langerhans' cells facilitates uptake of IgE-associated allergens and plays a pivotal role in the pathogenesis of atopic dermatitis. Seminal results from studies investigating Langerhans' cell Fc?RI in skin biopsy sections or epidermal cell suspensions demonstrate the highest receptor expression in lesional skin of patients with active atopic dermatitis.
Objective: We sought to investigate and localize Fc?RI expression on Langerhans' cells within a minimally disturbed tissue environment in clinically uninvolved skin and to compare receptor expression between healthy donors and patients with atopic dermatitis or other allergic diseases.
Methods: Intact epidermal sheets from skin suction blisters, immunofluorescently stained with Langerhans' cell markers and anti-Fc?RI? (mAbs 15E5 and 22E7) or anti-IgE, were examined by means of confocal microscopy. Samples incubated with anti-Fc?RI? before or after cell fixation-permeabilization were compared to discriminate between cytoplasmic and membrane localization.
Results: Cytoplasmic Fc?RI ? chain was found in Langerhans' cells from all donors, irrespective of atopic status. Surface Fc?RI-bound IgE was detected in the skin of individuals with active atopic dermatitis and in the skin of those with active asthma or rhinitis. No surface Fc?RI was expressed in the skin of patients with a clinical history of atopic dermatitis, asthma, or rhinitis whose disease was in remission or in the skin of nonatopic individuals.
Conclusion: In clinically uninvolved skin, Langerhans' cell-surface Fc?RI expression is not only linked to atopic dermatitis but is also generally associated with allergic disease. This supports the concept of a systemic regulatory mechanism associated with active allergic disease, which is further aggravated by local inflammation in atopic skin lesions.

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Published date: 2003

Identifiers

Local EPrints ID: 27417
URI: http://eprints.soton.ac.uk/id/eprint/27417
ISSN: 0091-6749
PURE UUID: 6b04a983-0b12-4282-babd-ea80b7a3c521

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Date deposited: 27 Apr 2006
Last modified: 15 Mar 2024 07:18

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Contributors

Author: Amanda E. Semper
Author: Kyle Heron
Author: Alexander C.S. Woollard
Author: Jarema P. Kochan
Author: Peter S. Friedmann
Author: Martin K. Church
Author: Ilona G. Reischl

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