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Bronchial angiogenesis in severe glucocorticoid-dependent asthma

Bronchial angiogenesis in severe glucocorticoid-dependent asthma
Bronchial angiogenesis in severe glucocorticoid-dependent asthma
To examine the role of the bronchial microvasculature and adhesion molecule expression in severe asthma, the authors have performed an immunohistochemical study on bronchial biopsies comparing 15 glucocorticoid-dependent asthmatics, 15 mild asthmatics and eight control subjects. Serially cut glycol methacrylate-embedded sections were stained with monoclonal antibodies identifying the vessel marker EN-4, intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1, E- and P-selectin. Sections were also stained for lymphocyte function associated antigen (LFA)-1 and very late antigen (VLA)-4. By comparison with mild asthma and nonasthma, severe asthma was characterized by increased numbers of submucosal vessels (p=0.009) which was associated with increased numbers of vessels expressing ICAM-1 (p=0.005). A highly significant correlation was found between the total number of EN-4+ vessels and the vessels expressing ICAM-1 (r=0.85, p=0.01). In contrast, E-selectin expression was lower in severe as compared with mild asthma (p=0.01) but not different from normal. No differences were found between the three groups in the expression of VCAM-1 and P-selectin nor in numbers of LFA-1+ and VLA-4+ cells. The results of this study support the notion that mucosal neovascularization is an important feature of airways remodelling in severe asthma. This is associated with a relatively higher density of vessels expressing intercellular adhesion molecule-1, although the expression of this adhesion molecule per vessel was not raised.
0903-1936
1014-1021
Vrugt, B.
d4c28d43-9943-42f4-b9f4-d3c082a6a2e4
Wilson, S.
21c6875d-6870-441b-ae7a-603562a646b8
Bron, A.
e0354c49-ecc0-411b-9b75-a9c5e3dc5837
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Djukanovic, R.
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Aalbers, R.
d505a4b7-d33b-463e-a807-1c1a1cbb8896
Vrugt, B.
d4c28d43-9943-42f4-b9f4-d3c082a6a2e4
Wilson, S.
21c6875d-6870-441b-ae7a-603562a646b8
Bron, A.
e0354c49-ecc0-411b-9b75-a9c5e3dc5837
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Djukanovic, R.
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Aalbers, R.
d505a4b7-d33b-463e-a807-1c1a1cbb8896

Vrugt, B., Wilson, S., Bron, A., Holgate, S.T., Djukanovic, R. and Aalbers, R. (2000) Bronchial angiogenesis in severe glucocorticoid-dependent asthma. European Respiratory Journal, 15 (6), 1014-1021.

Record type: Article

Abstract

To examine the role of the bronchial microvasculature and adhesion molecule expression in severe asthma, the authors have performed an immunohistochemical study on bronchial biopsies comparing 15 glucocorticoid-dependent asthmatics, 15 mild asthmatics and eight control subjects. Serially cut glycol methacrylate-embedded sections were stained with monoclonal antibodies identifying the vessel marker EN-4, intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1, E- and P-selectin. Sections were also stained for lymphocyte function associated antigen (LFA)-1 and very late antigen (VLA)-4. By comparison with mild asthma and nonasthma, severe asthma was characterized by increased numbers of submucosal vessels (p=0.009) which was associated with increased numbers of vessels expressing ICAM-1 (p=0.005). A highly significant correlation was found between the total number of EN-4+ vessels and the vessels expressing ICAM-1 (r=0.85, p=0.01). In contrast, E-selectin expression was lower in severe as compared with mild asthma (p=0.01) but not different from normal. No differences were found between the three groups in the expression of VCAM-1 and P-selectin nor in numbers of LFA-1+ and VLA-4+ cells. The results of this study support the notion that mucosal neovascularization is an important feature of airways remodelling in severe asthma. This is associated with a relatively higher density of vessels expressing intercellular adhesion molecule-1, although the expression of this adhesion molecule per vessel was not raised.

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Published date: 2000

Identifiers

Local EPrints ID: 27467
URI: https://eprints.soton.ac.uk/id/eprint/27467
ISSN: 0903-1936
PURE UUID: 1b234bbe-4b68-495e-9986-d035d49927c0
ORCID for R. Djukanovic: ORCID iD orcid.org/0000-0001-6039-5612

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Date deposited: 26 Apr 2006
Last modified: 17 Jul 2019 01:22

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