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Characterization of ciliated bronchial epithelium 1, a ciliated cell-associated gene induced during mucociliary differentiation

Characterization of ciliated bronchial epithelium 1, a ciliated cell-associated gene induced during mucociliary differentiation
Characterization of ciliated bronchial epithelium 1, a ciliated cell-associated gene induced during mucociliary differentiation
Lung epithelial structure is altered in asthma; however, the precise mechanisms underlying epithelial repair, including differentiation from basal to columnar epithelial cells, are not well defined. In the course of random sequencing of a cDNA library from human lung biopsies, we have identified a novel gene, ciliated bronchial epithelium 1 (CBE1). Expression of CBE1 was induced during in vitro differentiation of bronchial epithelial cells. Synchronous expression with tektin and hepatocyte nuclear factor 3/forkhead homologue 4, down-regulation by interleukin-13, and its tissue distribution strongly suggested that CBE1 is associated with ciliated cells. Two isoforms of the 0.7-kb full-length cDNA were identified, resulting in open reading frames with different carboxyl termini, with no homology to known proteins. Expression of CBE1 in ciliated epithelial cells was confirmed by immunohistochemistry. Quantitative reverse transcription–polymerase chain reaction analysis using bronchial biopsies showed no difference of expression of CBE1 between normal subjects and subjects with asthma. Expression studies showed that CBE1 is nuclear- or perinuclear-localized, depending on cell type. Regulated expression during differentiation and the subcellular localization of CBE1 suggest that it may play an important role in the differentiation and/or function of ciliated cells in human airways.
1044-1549
491-500
Yoshisue, Hajime
903ea8ba-9ef3-4180-a465-8fc27d84c11b
Puddicombe, Sarah M.
124e2c4e-ab9a-46f3-855c-b54ed0b61cc4
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Haitchi, Hans-Michael
68dadb29-305d-4236-884f-e9c93f4d78fe
Powell, Robert M.
884d6594-3f50-4be5-9516-d64b29dad63d
Wilson, David I.
1500fca1-7082-4271-95f4-691f1d1252a2
Pandit, Anita
a6cd7096-5cef-438a-a7bc-060efad3c235
Berger, Ann E.
af4cfd91-83cb-46c2-bca7-a955f82c3a56
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Yoshisue, Hajime
903ea8ba-9ef3-4180-a465-8fc27d84c11b
Puddicombe, Sarah M.
124e2c4e-ab9a-46f3-855c-b54ed0b61cc4
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Haitchi, Hans-Michael
68dadb29-305d-4236-884f-e9c93f4d78fe
Powell, Robert M.
884d6594-3f50-4be5-9516-d64b29dad63d
Wilson, David I.
1500fca1-7082-4271-95f4-691f1d1252a2
Pandit, Anita
a6cd7096-5cef-438a-a7bc-060efad3c235
Berger, Ann E.
af4cfd91-83cb-46c2-bca7-a955f82c3a56
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc

Yoshisue, Hajime, Puddicombe, Sarah M., Wilson, Susan J., Haitchi, Hans-Michael, Powell, Robert M., Wilson, David I., Pandit, Anita, Berger, Ann E., Davies, Donna E., Holloway, John W. and Holgate, Stephen T. (2004) Characterization of ciliated bronchial epithelium 1, a ciliated cell-associated gene induced during mucociliary differentiation. American Journal of Respiratory Cell and Molecular Biology, 31 (5), 491-500. (doi:10.1165/rcmb.2004-0050OC).

Record type: Article

Abstract

Lung epithelial structure is altered in asthma; however, the precise mechanisms underlying epithelial repair, including differentiation from basal to columnar epithelial cells, are not well defined. In the course of random sequencing of a cDNA library from human lung biopsies, we have identified a novel gene, ciliated bronchial epithelium 1 (CBE1). Expression of CBE1 was induced during in vitro differentiation of bronchial epithelial cells. Synchronous expression with tektin and hepatocyte nuclear factor 3/forkhead homologue 4, down-regulation by interleukin-13, and its tissue distribution strongly suggested that CBE1 is associated with ciliated cells. Two isoforms of the 0.7-kb full-length cDNA were identified, resulting in open reading frames with different carboxyl termini, with no homology to known proteins. Expression of CBE1 in ciliated epithelial cells was confirmed by immunohistochemistry. Quantitative reverse transcription–polymerase chain reaction analysis using bronchial biopsies showed no difference of expression of CBE1 between normal subjects and subjects with asthma. Expression studies showed that CBE1 is nuclear- or perinuclear-localized, depending on cell type. Regulated expression during differentiation and the subcellular localization of CBE1 suggest that it may play an important role in the differentiation and/or function of ciliated cells in human airways.

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Published date: November 2004

Identifiers

Local EPrints ID: 27506
URI: https://eprints.soton.ac.uk/id/eprint/27506
ISSN: 1044-1549
PURE UUID: 39823af2-8553-46d2-b7ac-f97d55f8f29a
ORCID for Hans-Michael Haitchi: ORCID iD orcid.org/0000-0001-8603-302X
ORCID for John W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

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Date deposited: 26 Apr 2006
Last modified: 24 Jul 2018 00:37

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Contributors

Author: Hajime Yoshisue
Author: Sarah M. Puddicombe
Author: Susan J. Wilson
Author: Robert M. Powell
Author: David I. Wilson
Author: Anita Pandit
Author: Ann E. Berger
Author: Donna E. Davies

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