The University of Southampton
University of Southampton Institutional Repository

4-Hydroxynonenal immunoreactivity is increased in human hippocampus after global ischemia

McKracken, Eileen, Graham, David I., Nilsen, Margaret, Stewart, Janice, Nicoll, James A.R. and Horsburgh, Karen (2001) 4-Hydroxynonenal immunoreactivity is increased in human hippocampus after global ischemia Brain Pathology, 11, (4), pp. 414-421.

Record type: Article


Oxidative stress and lipid peroxidation may contribute to the pathology of neurodegenerative disorders such as Alzheimer’s disease (AD) and cerebral ischemia. 4-Hydroxynonenal (4-HNE) is a toxic by-product of lipid peroxidation, and immunoreactivity to 4-HNE has been used to examine lipid peroxidation in the pathogenesis of AD and ischemia. This study sought to determine 1) if there are cellular alterations in 4-HNE immunoreactivity in the human hippocampus after global ischemia, and 2) whether possession of an apolipoprotein E (APOE) e4 allele influenced the extent of 4-HNE immunoreactivity. 4-HNE immunoreactivity was assessed semi-quantitatively in the temporal lobe of a group of controls (n = 44) and in a group of patients who had an episode of global ischemia as a result of a cardiorespiratory arrest and subsequently died (n = 56, survival ranged from 1hr to 42days). There was minimal cellular 4-HNE immunoreactivity in the control group. However, compared to controls, 4-HNE immunoreactivity was significantly increased in neurons (p , 0.0002) and glia (p , 0.0001) in the hippocampal formation after global ischemia. Possession of an APOE e4 allele did not influence the extent of neuronal or glial 4-HNE immunostaining in the control or global ischemia group. There was a significant negative correlation between the extent of neuronal 4-HNE immunoreactivity with survival period after global ischemia (r2 = 0.0801; p , 0.036) and a significant positive correlation between the extent of glial 4-HNE immunoreactivity and survival after global ischemia (r2 = 0.2958; p , 0.0001). The data indicate a marked increase in neuronal and glial 4-HNE. This substantiates a role for lipid peroxidation in the pathogenesis of cerebral ischemia. There was no indication that APOE genotype influenced the extent of 4-HNE immunoreactivity.

Full text not available from this repository.

More information

Published date: 2001


Local EPrints ID: 27657
PURE UUID: 7fcd5551-9003-4c73-af70-eba352e01958
ORCID for James A.R. Nicoll: ORCID iD

Catalogue record

Date deposited: 27 Apr 2006
Last modified: 17 Jul 2017 16:04

Export record


Author: Eileen McKracken
Author: David I. Graham
Author: Margaret Nilsen
Author: Janice Stewart
Author: Karen Horsburgh

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.